Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   IQ863_RS00890 Genome accession   NZ_CP064270
Coordinates   144859..145281 (-) Length   140 a.a.
NCBI ID   WP_013057949.1    Uniprot ID   A0A8D3X0F8
Organism   Priestia megaterium strain N447-3     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 139859..150281
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IQ863_RS00860 - 140156..140641 (+) 486 WP_243556495.1 hypothetical protein -
  IQ863_RS00865 - 140775..141320 (-) 546 WP_115654369.1 GNAT family N-acetyltransferase -
  IQ863_RS00870 - 141620..142897 (+) 1278 WP_243556497.1 glycosyltransferase -
  IQ863_RS00875 - 142921..143577 (+) 657 WP_014459240.1 TetR/AcrR family transcriptional regulator -
  IQ863_RS00880 - 143731..144324 (+) 594 WP_115654367.1 hypothetical protein -
  IQ863_RS00885 - 144365..144709 (-) 345 WP_014459242.1 DUF1516 family protein -
  IQ863_RS00890 nucA/comI 144859..145281 (-) 423 WP_013057949.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  IQ863_RS00895 - 145422..145886 (-) 465 WP_014459243.1 FixH family protein -
  IQ863_RS00900 - 145916..147298 (-) 1383 WP_209367038.1 PepSY domain-containing protein -
  IQ863_RS00905 - 147436..148077 (-) 642 WP_140451349.1 response regulator transcription factor -
  IQ863_RS00910 - 148078..148899 (-) 822 WP_140451350.1 histidine kinase -
  IQ863_RS00915 - 149120..149953 (+) 834 WP_209367036.1 AraC family transcriptional regulator -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 15329.17 Da        Isoelectric Point: 4.4990

>NTDB_id=500430 IQ863_RS00890 WP_013057949.1 144859..145281(-) (nucA/comI) [Priestia megaterium strain N447-3]
MKKIVSGILILVLAVIVMIEGNPLEQNSSTNTSYDEVITFPADRYPETAAHIQHAIDKGKSAVCTIDRDGAEENRRESLK
GIPTKSGYDRDEFPMAFCEEGGSGADIEYVKPSDNRGAGSWISHQVDEFPDGTKVLIEVK

Nucleotide


Download         Length: 423 bp        

>NTDB_id=500430 IQ863_RS00890 WP_013057949.1 144859..145281(-) (nucA/comI) [Priestia megaterium strain N447-3]
TTGAAAAAAATTGTTTCTGGTATTTTAATTTTAGTATTAGCCGTTATTGTTATGATTGAAGGAAATCCGTTAGAGCAGAA
CTCTTCAACGAATACCTCCTACGATGAAGTAATTACCTTCCCTGCTGATCGTTACCCTGAAACTGCAGCGCACATTCAAC
ATGCAATCGATAAAGGAAAATCGGCTGTTTGTACAATCGACCGAGACGGGGCTGAGGAAAACCGCCGTGAGTCTTTAAAA
GGCATTCCGACTAAAAGCGGCTATGACCGAGATGAGTTTCCGATGGCCTTTTGTGAAGAAGGCGGAAGCGGTGCAGATAT
TGAATATGTAAAGCCTTCAGACAACCGAGGCGCCGGTTCTTGGATTTCACATCAAGTAGATGAGTTTCCAGACGGTACGA
AGGTTTTAATTGAAGTGAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(43-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

61.207

82.857

0.507