Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   IQ680_RS13365 Genome accession   NZ_CP064266
Coordinates   2592224..2595739 (+) Length   1171 a.a.
NCBI ID   WP_243521099.1    Uniprot ID   -
Organism   Bacillus pseudomycoides strain N451-2     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2587224..2600739
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IQ680_RS13345 - 2588326..2588589 (+) 264 WP_396124441.1 DDE-type integrase/transposase/recombinase -
  IQ680_RS13350 - 2588645..2590432 (+) 1788 WP_243521097.1 PAS domain-containing sensor histidine kinase -
  IQ680_RS13355 - 2590832..2591425 (+) 594 WP_243521098.1 TVP38/TMEM64 family protein -
  IQ680_RS13360 lepB 2591483..2592046 (+) 564 WP_098337492.1 signal peptidase I -
  IQ680_RS13365 addB 2592224..2595739 (+) 3516 WP_243521099.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  IQ680_RS13370 addA 2595736..2599461 (+) 3726 WP_243521100.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  IQ680_RS13375 - 2599537..2599830 (+) 294 WP_098337489.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  IQ680_RS13380 gerPF 2599916..2600131 (-) 216 WP_098337488.1 spore germination protein GerPF -
  IQ680_RS13385 - 2600177..2600563 (-) 387 WP_243521101.1 spore germination protein GerPE -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134128.98 Da        Isoelectric Point: 5.1420

>NTDB_id=500411 IQ680_RS13365 WP_243521099.1 2592224..2595739(+) (addB) [Bacillus pseudomycoides strain N451-2]
MSLRFVIGRAGSGKSRFCLHEVQEELKQRPRGETILYLVPEQMTFQTQQALIGNEEVSGSIRAQVFSFSRLAWKVLQEVG
GMSRLHIDEAGVHMLLRKIVEARKEGLYVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSGDEQKLLA
NKIYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSDYVKGAEIYIDGFHSFSPQELEIIRQLMICGARITVTLTVDE
KTLAQPVSELDLFYQTVLTYEKVKQLAREEKIAVENTIPMREQPRFHSPALAHLEAEFETRPSKKFQSETDITLYTAANL
RAEVEGVAREIRRLVAEEGYRYRDVAVLLRNGESYYDVMKTLFTDYDIPHFIDEKRPMSHHPLIECIRSALEVISGNWRY
DAVFRCIKTELLYPSDVRKEVMREEMDEFENYCLAYGVQGKRWTADEPWTYRRYRSLDGADGTQTDSEREMEEKINRLRD
VVRTPIIRLQKRFKRATSVVQMCEAMYLFLEELEVPKKLEDLRMRAEAEGNFLFASDHDQVWEEVMNLLDTFVEMLGEEK
MSRAMFIDVITTGLEALQFANIPPSLDQVLIANIDRSRLSGVRAAFVIGANEGIIPAAPSDEGMLSDEERESLATAGVEL
APTTRQTLLEEQFVIYQTVTRASERLYISCPLADEEGKTLLSSSFIKKVKRMFPSVKEVFITNDVNDLSRQAQISYVATP
EVTLSYVTQQLQNWKRYGFEGNLDFWWDVYNFYVTSSEWKQKSSRVLSSLFYRNHAQKLSPVVSRDLYGDTIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKKIADKLLRENRTWADLSIKECEHLSAVVIEEIAPLLQRQIL
LSSNRNHYLKQKLQQIIFRTSLILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFTLANGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSTKALDLTEVYYGLALQMLTYLDVVISNANTWMKKDQAVSPAGVLYFHIHNPIVEMKGDASEEEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFTTLQQYVHHTFENIGKDITEGVIDIAP
YKMGNKAACTFCNFRSVCQFDESLEDNQFRALKDMKDSEAMEKMRGEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=500411 IQ680_RS13365 WP_243521099.1 2592224..2595739(+) (addB) [Bacillus pseudomycoides strain N451-2]
ATGTCGCTTCGATTTGTGATCGGACGGGCTGGAAGTGGAAAAAGTAGATTTTGTTTACATGAAGTACAAGAAGAATTAAA
ACAGCGTCCAAGAGGCGAAACGATTTTATATCTTGTACCGGAACAAATGACGTTCCAAACGCAGCAAGCATTAATAGGAA
ATGAAGAAGTCAGTGGCTCTATTCGGGCGCAAGTGTTTAGTTTTTCAAGGTTAGCGTGGAAGGTTTTGCAAGAAGTTGGT
GGCATGAGTCGTCTTCATATTGATGAGGCGGGCGTACATATGCTACTGCGCAAAATTGTAGAGGCGCGTAAGGAAGGATT
ATATGTGTTTCAAAAAGCGGCAGAACAAAATGGCTTTTTTGAACATTTAGGAAGCATGATTGCTGAATTTAAACGTTATA
ATGTAACGCCATCAAATGTATATGAGATGTGGCAACAGTTAGATGCACACTCAAGCGGTGATGAGCAAAAGTTATTAGCA
AATAAAATATATGACCTTCAGCTTTTATATGATGATTTTGAACGGGCATTAATTGGTAAATATTTAGATTCAGAAGATTA
TTTACAGTTGCTTGTCGAAAAGCTTCCGCAGTCTGACTATGTAAAAGGTGCGGAAATTTATATAGATGGATTTCATTCGT
TTTCCCCGCAGGAATTAGAAATTATAAGGCAGCTTATGATTTGCGGAGCAAGAATAACAGTTACGTTAACGGTAGATGAG
AAAACGTTAGCGCAACCAGTAAGTGAACTAGATTTGTTTTATCAAACAGTCCTTACGTATGAAAAAGTAAAACAGTTAGC
ACGTGAAGAGAAAATCGCGGTTGAAAATACGATTCCGATGAGAGAACAGCCGCGTTTTCATTCACCGGCACTAGCACATC
TAGAAGCGGAGTTTGAAACTCGTCCTTCTAAGAAGTTTCAAAGTGAAACAGATATTACATTATATACAGCGGCAAATTTG
CGGGCGGAAGTGGAAGGAGTAGCACGTGAAATTCGCAGACTTGTTGCAGAAGAGGGTTATCGCTACCGAGATGTTGCGGT
TCTTCTTCGTAATGGAGAAAGCTATTATGATGTCATGAAAACGTTGTTTACGGATTATGATATTCCGCATTTTATTGATG
AGAAGCGTCCAATGTCGCATCATCCACTCATTGAATGTATTCGTTCTGCGCTAGAGGTCATTAGTGGGAATTGGCGTTAT
GATGCAGTATTCCGCTGTATAAAAACGGAACTATTATATCCGTCAGATGTAAGAAAAGAAGTTATGCGTGAAGAAATGGA
TGAGTTTGAAAATTACTGTTTAGCATATGGCGTACAAGGAAAAAGATGGACGGCAGATGAACCGTGGACATATCGTCGCT
ATCGTTCGTTAGATGGGGCGGACGGAACACAAACAGATAGCGAACGTGAAATGGAAGAAAAAATCAATCGTCTTCGTGAT
GTGGTACGAACACCGATTATTCGTTTGCAAAAGCGTTTCAAACGTGCAACCTCAGTTGTGCAAATGTGTGAAGCAATGTA
TTTATTTTTAGAAGAATTAGAAGTTCCTAAAAAACTGGAAGATTTGCGTATGCGTGCTGAAGCAGAGGGGAATTTCTTAT
TTGCAAGTGACCATGATCAAGTTTGGGAAGAGGTAATGAATCTTCTTGATACATTTGTAGAAATGCTAGGCGAAGAAAAG
ATGTCTCGTGCAATGTTTATCGATGTAATAACAACTGGACTTGAGGCACTTCAATTTGCAAATATCCCACCGTCTTTAGA
TCAAGTGCTGATTGCAAATATTGATCGATCAAGGTTATCGGGTGTTCGTGCGGCCTTTGTAATTGGTGCGAATGAAGGAA
TTATTCCAGCAGCTCCTTCAGATGAAGGCATGTTATCAGATGAAGAAAGAGAATCTCTCGCAACAGCTGGTGTAGAATTA
GCACCAACAACAAGGCAAACTTTATTAGAAGAGCAGTTTGTTATTTATCAAACGGTAACTCGTGCATCTGAGAGACTATA
TATTTCTTGTCCGCTTGCGGATGAAGAAGGAAAGACGTTACTTTCATCTAGCTTTATTAAGAAAGTGAAAAGAATGTTTC
CTTCTGTCAAAGAAGTGTTCATAACGAATGATGTGAATGATTTATCTCGTCAGGCGCAAATTTCGTACGTAGCAACGCCA
GAGGTTACATTGTCATATGTAACGCAGCAATTACAAAACTGGAAACGATATGGTTTTGAAGGGAATCTTGATTTTTGGTG
GGATGTATACAACTTCTATGTGACTTCATCGGAATGGAAGCAAAAAAGTAGTCGCGTGTTATCTAGTTTATTCTATCGAA
ACCATGCGCAGAAACTTAGCCCAGTTGTAAGTAGAGATCTATACGGAGATACAATCAAAGGGAGCGTCTCTCGTATGGAA
CTATTTAATCGCTGCGCATATGCTCATTTTGCACAGCACGGATTATCGCTAAGGGAGCGTGATATTTTCAAATTAGATGC
ACCGGATATCGGCGAATTATTCCATGCGGCATTAAAGAAAATTGCAGATAAGTTACTCAGAGAAAATCGTACTTGGGCTG
ATTTATCGATAAAAGAGTGTGAGCATCTTTCTGCGGTAGTAATTGAAGAAATAGCACCGCTACTGCAAAGACAAATTTTA
TTAAGTTCAAATCGAAATCATTATTTAAAACAAAAATTACAGCAAATCATTTTCCGTACATCGCTTATCCTTCGTGAACA
TGCAAAGTCTAGCGGGTTTGTACCAGTTGATTTAGAAGTTCCATTCGGTATGGGTGGCACAGGTTCACTTCCGCCGATGG
AGTTTACACTCGCAAATGGTGTGAAAATGGAAGTTGTTGGCCGCATTGACCGTGTAGATAAAGCGGAAGATGAAAACGGT
ACGTTCCTTCGCATTATTGATTATAAATCGAGCACAAAAGCGTTAGATTTAACGGAAGTGTATTATGGTTTAGCGCTTCA
AATGTTAACGTATTTAGATGTTGTCATTTCAAATGCGAATACGTGGATGAAAAAGGATCAAGCGGTATCACCTGCTGGCG
TTCTATATTTCCATATTCATAACCCAATTGTGGAGATGAAGGGCGATGCTTCAGAAGAAGAGATTGAAAAGGAAATTTTA
AAGAAATTCAAAATGAAAGGTCTCGTGCTAGGAGATGCGGATGTTGTTCGTTTAATGGACAATAAGTTAAGTACGGGAAG
TTCAGATATTATTTCTGCCGGTCTGAAAAAAGATGGTAGTTTTAGTGCGCGTTCTAGTATTGCAAGTGAACAAGAATTTA
CAACCTTACAGCAGTATGTACATCATACATTTGAAAACATTGGGAAAGACATTACAGAAGGTGTCATTGATATTGCACCG
TATAAAATGGGGAATAAAGCAGCTTGTACGTTCTGTAATTTCCGCTCTGTTTGTCAGTTTGATGAATCGCTTGAAGACAA
TCAGTTCCGCGCGTTAAAAGATATGAAAGACAGTGAAGCGATGGAGAAGATGAGAGGGGAGGTTGGTGGCGAATGA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)

UvrD-like helicase C-terminal domain (UvrD_C)

(305-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.147

100

0.492