Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KKZ13_RS12435 Genome accession   NZ_CP075737
Coordinates   2579226..2579903 (+) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain CPF6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2574226..2584903
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KKZ13_RS12405 (KKZ13_12375) ltrA 2574444..2575952 (+) 1509 WP_001189111.1 group II intron reverse transcriptase/maturase -
  KKZ13_RS12410 (KKZ13_12380) - 2576033..2576522 (+) 490 Protein_2435 IS3 family transposase -
  KKZ13_RS12415 (KKZ13_12385) - 2576531..2576614 (+) 84 Protein_2436 type II secretion system protein J -
  KKZ13_RS12420 (KKZ13_12390) gspK 2576607..2577590 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  KKZ13_RS12425 (KKZ13_12395) gspL 2577605..2578768 (+) 1164 WP_089075207.1 type II secretion system protein GspL -
  KKZ13_RS12430 (KKZ13_12400) gspM 2578765..2579226 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  KKZ13_RS12435 (KKZ13_12405) pilD 2579226..2579903 (+) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  KKZ13_RS12440 (KKZ13_12410) bfr 2579932..2580408 (-) 477 WP_000675504.1 bacterioferritin -
  KKZ13_RS12445 (KKZ13_12415) bfd 2580480..2580674 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  KKZ13_RS12450 (KKZ13_12420) chiA 2580843..2583536 (-) 2694 WP_089075206.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=500078 KKZ13_RS12435 WP_000178161.1 2579226..2579903(+) (pilD) [Escherichia coli strain CPF6]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=500078 KKZ13_RS12435 WP_000178161.1 2579226..2579903(+) (pilD) [Escherichia coli strain CPF6]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378