Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   IS507_RS02650 Genome accession   NZ_CP064194
Coordinates   545267..546067 (+) Length   266 a.a.
NCBI ID   WP_004738086.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain X4-65     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 540267..551067
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IS507_RS02625 - 541177..542475 (+) 1299 WP_279446990.1 glycosyltransferase family 1 protein -
  IS507_RS02630 - 542500..543051 (+) 552 WP_000669565.1 phosphatase PAP2 family protein -
  IS507_RS02635 iscX 543151..543351 (+) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  IS507_RS02640 ndk 543465..543896 (+) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  IS507_RS02645 rlmN 544019..545251 (+) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  IS507_RS02650 pilF 545267..546067 (+) 801 WP_004738086.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  IS507_RS02655 - 546058..546849 (+) 792 WP_032061256.1 RodZ domain-containing protein -
  IS507_RS02660 ispG 546871..547986 (+) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  IS507_RS02665 hisS 547983..549275 (+) 1293 WP_000095264.1 histidine--tRNA ligase -
  IS507_RS02670 - 549304..550002 (+) 699 WP_000058788.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30094.44 Da        Isoelectric Point: 9.2307

>NTDB_id=500042 IS507_RS02650 WP_004738086.1 545267..546067(+) (pilF) [Acinetobacter baumannii strain X4-65]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=500042 IS507_RS02650 WP_004738086.1 545267..546067(+) (pilF) [Acinetobacter baumannii strain X4-65]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCAGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

100

100

1
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726