Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KKZ10_RS01925 Genome accession   NZ_CP075722
Coordinates   416735..417400 (-) Length   221 a.a.
NCBI ID   WP_000178160.1    Uniprot ID   -
Organism   Escherichia coli strain CPE35     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 411735..422400
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KKZ10_RS01910 (KKZ10_01900) chiA 413090..415783 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  KKZ10_RS01915 (KKZ10_01905) bfd 415952..416146 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  KKZ10_RS01920 (KKZ10_01910) bfr 416218..416694 (+) 477 WP_000675504.1 bacterioferritin -
  KKZ10_RS01925 (KKZ10_01915) pilD 416735..417400 (-) 666 WP_000178160.1 prepilin peptidase GspO Machinery gene
  KKZ10_RS01930 (KKZ10_01920) gspM 417400..417861 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  KKZ10_RS01935 (KKZ10_01925) gspL 417858..419021 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  KKZ10_RS01940 (KKZ10_01930) gspK 419036..420019 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  KKZ10_RS01945 (KKZ10_01935) gspJ 420012..420599 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  KKZ10_RS01950 (KKZ10_01940) gspI 420592..420969 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  KKZ10_RS01955 (KKZ10_01945) gspH 420966..421475 (-) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  KKZ10_RS01960 (KKZ10_01950) gspG 421483..421920 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 221 a.a.        Molecular weight: 24349.16 Da        Isoelectric Point: 7.9585

>NTDB_id=499891 KKZ10_RS01925 WP_000178160.1 416735..417400(-) (pilD) [Escherichia coli strain CPE35]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLA

Nucleotide


Download         Length: 666 bp        

>NTDB_id=499891 KKZ10_RS01925 WP_000178160.1 416735..417400(-) (pilD) [Escherichia coli strain CPE35]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTAG

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.389