Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   IRA68_RS01900 Genome accession   NZ_CP064097
Coordinates   369550..370602 (-) Length   350 a.a.
NCBI ID   WP_028411611.1    Uniprot ID   -
Organism   Priestia aryabhattai strain B2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 364550..375602
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IRA68_RS01860 - 365566..366390 (+) 825 WP_221832016.1 YqhG family protein -
  IRA68_RS01865 - 366414..366599 (-) 186 WP_013059170.1 YqzE family protein -
  IRA68_RS01870 comGG 366643..367017 (-) 375 WP_033579700.1 competence type IV pilus minor pilin ComGG -
  IRA68_RS01875 comGF 367007..367444 (-) 438 WP_243495158.1 competence type IV pilus minor pilin ComGF -
  IRA68_RS01880 - 367441..367779 (-) 339 WP_243495159.1 type II secretion system protein -
  IRA68_RS01885 comGD 367763..368200 (-) 438 WP_016765559.1 competence type IV pilus minor pilin ComGD -
  IRA68_RS01890 comGC 368187..368498 (-) 312 WP_016765560.1 competence type IV pilus major pilin ComGC -
  IRA68_RS01895 comGB 368513..369559 (-) 1047 WP_033579696.1 competence type IV pilus assembly protein ComGB -
  IRA68_RS01900 comGA 369550..370602 (-) 1053 WP_028411611.1 competence type IV pilus ATPase ComGA Machinery gene
  IRA68_RS01905 - 370871..371254 (-) 384 WP_243495160.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  IRA68_RS01910 - 371516..371752 (+) 237 WP_013059179.1 DUF2626 domain-containing protein -
  IRA68_RS01915 - 371790..372422 (-) 633 WP_243495161.1 MBL fold metallo-hydrolase -
  IRA68_RS01920 - 372632..372808 (+) 177 WP_028411614.1 DUF2759 domain-containing protein -
  IRA68_RS01925 - 372844..373746 (-) 903 WP_243495162.1 M14 family metallocarboxypeptidase -
  IRA68_RS01930 - 373858..374826 (-) 969 WP_013059183.1 ROK family glucokinase -
  IRA68_RS01935 - 374829..375035 (-) 207 WP_028411617.1 YqgQ family protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39596.97 Da        Isoelectric Point: 9.0586

>NTDB_id=499528 IRA68_RS01900 WP_028411611.1 369550..370602(-) (comGA) [Priestia aryabhattai strain B2]
MTEIEQFATYVIRDACECRSSDVHIVPRKKDAVIQLRIDQELILKRVISKEMSERLISHFKFLGGMDIGERRKPQNGALT
TSVDHYQVNLRLSTLPTSHDESLVIRLLPHQQSTSLKHLSLFPEHTKKLISLLKFSHGLIIFTGPTGSGKTTTLYTLLHE
VKHLFNRNVITLEDPIEKPSETVLQVQVNERAGITYTTGLKAILRHDPDIIMVGEIRDSETAKIAVRAALTGHLVLTTMH
TKDTKGSLYRLLEFGVSFQEMEQTLVAVAAQRLVEIRCPLCSGKCHPACKKMRKHRQSSIYELLYGKELSAVMREVKGEN
ADYDYKTLRDVILKGIALGYLYPHSLDGWG

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=499528 IRA68_RS01900 WP_028411611.1 369550..370602(-) (comGA) [Priestia aryabhattai strain B2]
TTGACCGAAATTGAACAGTTTGCTACTTACGTTATACGAGATGCATGTGAGTGTCGAAGTTCTGATGTGCATATTGTGCC
CCGAAAGAAAGATGCTGTGATTCAGCTGCGAATTGACCAGGAGCTTATTTTAAAGAGGGTTATTTCAAAGGAAATGTCTG
AACGGCTCATTTCGCATTTTAAATTTTTAGGGGGAATGGATATTGGGGAGCGCAGAAAACCTCAAAATGGTGCATTGACC
ACTTCCGTTGATCACTATCAAGTAAATTTGCGTTTATCAACTCTTCCTACTTCTCATGATGAAAGTCTTGTTATTCGGCT
TTTGCCCCATCAACAATCTACCTCTCTCAAACATTTATCTCTTTTTCCTGAACATACTAAAAAGCTTATTTCTCTCTTGA
AATTCTCTCATGGATTAATTATTTTTACCGGTCCTACAGGATCAGGTAAAACCACTACTCTATATACTCTTTTACACGAA
GTAAAGCACCTTTTTAATCGAAATGTTATTACACTAGAAGATCCTATTGAAAAACCGAGTGAAACCGTGCTTCAAGTGCA
GGTAAATGAACGCGCAGGGATTACGTATACGACTGGTTTAAAAGCTATATTGCGCCATGATCCAGACATTATCATGGTGG
GAGAAATAAGAGATAGTGAGACCGCCAAAATTGCTGTGAGAGCAGCGCTTACCGGACACTTGGTTTTGACAACGATGCAT
ACTAAAGATACAAAAGGATCTTTGTACAGGCTGCTTGAGTTTGGCGTTAGCTTTCAAGAAATGGAGCAGACTCTTGTAGC
AGTAGCTGCACAGCGTCTTGTAGAAATTCGCTGTCCGCTGTGTTCGGGAAAATGCCACCCTGCTTGTAAAAAAATGAGGA
AGCATCGTCAGTCCAGCATATACGAATTGCTTTATGGCAAGGAGCTTAGCGCAGTGATGAGAGAAGTGAAAGGAGAAAAT
GCGGATTATGATTATAAGACGCTCCGAGATGTTATTCTAAAAGGAATCGCGCTAGGCTATTTATATCCGCATTCGCTAGA
CGGATGGGGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

58.571

100

0.586