Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   IRJ28_RS17140 Genome accession   NZ_CP064096
Coordinates   3266720..3267169 (+) Length   149 a.a.
NCBI ID   WP_003246436.1    Uniprot ID   A0AAE2SFQ0
Organism   Bacillus subtilis strain N1142-3at     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3261720..3272169
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IRJ28_RS17120 hxlR 3262930..3263292 (-) 363 WP_003246265.1 transcriptional activator HxlR -
  IRJ28_RS17125 hxlA 3263525..3264157 (+) 633 WP_003246452.1 3-hexulose-6-phosphate synthase -
  IRJ28_RS17130 hxlB 3264163..3264720 (+) 558 WP_003246343.1 6-phospho-3-hexuloisomerase -
  IRJ28_RS17135 tlpC 3264831..3266552 (+) 1722 WP_003246498.1 methyl-accepting chemotaxis protein TlpC -
  IRJ28_RS17140 nucA/comI 3266720..3267169 (+) 450 WP_003246436.1 DNA-entry nuclease Machinery gene
  IRJ28_RS17145 nin/comJ 3267196..3267594 (+) 399 WP_003246342.1 DNA-entry nuclease inhibitor Regulator
  IRJ28_RS17150 bglC 3267631..3269064 (-) 1434 WP_003246242.1 6-phospho-beta-glucosidase -
  IRJ28_RS17155 yckD 3269218..3269550 (-) 333 WP_003246247.1 YckD family protein -
  IRJ28_RS17160 yckC 3269632..3270087 (-) 456 WP_003246356.1 RDD family protein -
  IRJ28_RS17165 - 3270106..3270303 (-) 198 WP_119122841.1 hypothetical protein -
  IRJ28_RS17170 yckB 3270465..3271328 (+) 864 WP_009966510.1 transporter substrate-binding domain-containing protein -
  IRJ28_RS17175 yckA 3271338..3272018 (+) 681 WP_003234624.1 amino acid ABC transporter permease -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16486.48 Da        Isoelectric Point: 4.6274

>NTDB_id=499503 IRJ28_RS17140 WP_003246436.1 3266720..3267169(+) (nucA/comI) [Bacillus subtilis strain N1142-3at]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETMAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHRLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=499503 IRJ28_RS17140 WP_003246436.1 3266720..3267169(+) (nucA/comI) [Bacillus subtilis strain N1142-3at]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATGGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAAGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
GGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(53-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

100

100

1