Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   IRJ28_RS05715 Genome accession   NZ_CP064096
Coordinates   1079260..1080330 (+) Length   356 a.a.
NCBI ID   WP_004399124.1    Uniprot ID   A0A809FNW6
Organism   Bacillus subtilis strain N1142-3at     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1074260..1085330
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IRJ28_RS05685 yqgY 1074454..1074699 (-) 246 WP_003226300.1 DUF2626 domain-containing protein -
  IRJ28_RS05690 mgsR 1074931..1075311 (+) 381 WP_003230147.1 transcriptional regulator MgsR -
  IRJ28_RS05700 rsbRD 1075535..1076371 (+) 837 WP_004398754.1 RsbT co-antagonist protein RsbRD -
  IRJ28_RS05705 yqhB 1076424..1077752 (-) 1329 WP_009967730.1 hemolysin family protein -
  IRJ28_RS05710 corA 1077895..1078848 (+) 954 WP_004399136.1 magnesium transporter CorA -
  IRJ28_RS05715 comGA 1079260..1080330 (+) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  IRJ28_RS05720 comGB 1080317..1081354 (+) 1038 WP_009967727.1 comG operon protein ComGB Machinery gene
  IRJ28_RS05725 comGC 1081368..1081664 (+) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  IRJ28_RS05730 comGD 1081654..1082085 (+) 432 WP_004398628.1 comG operon protein ComGD Machinery gene
  IRJ28_RS05735 comGE 1082069..1082416 (+) 348 WP_003230165.1 ComG operon protein 5 Machinery gene
  IRJ28_RS05740 comGF 1082442..1082825 (+) 384 WP_003230168.1 ComG operon protein ComGF Machinery gene
  IRJ28_RS05745 comGG 1082826..1083200 (+) 375 WP_003230170.1 ComG operon protein ComGG Machinery gene
  IRJ28_RS05750 spoIIT 1083271..1083450 (+) 180 WP_003230176.1 YqzE family protein -
  IRJ28_RS05755 yqzG 1083492..1083818 (-) 327 WP_003246051.1 YqzG/YhdC family protein -
  IRJ28_RS05760 tapA 1084090..1084851 (+) 762 WP_004399106.1 amyloid fiber anchoring/assembly protein TapA -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40458.64 Da        Isoelectric Point: 9.0175

>NTDB_id=499457 IRJ28_RS05715 WP_004399124.1 1079260..1080330(+) (comGA) [Bacillus subtilis strain N1142-3at]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=499457 IRJ28_RS05715 WP_004399124.1 1079260..1080330(+) (comGA) [Bacillus subtilis strain N1142-3at]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATTGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGACTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGTTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCGACTGGTTCAGGGAAGACTACCACATTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATCGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGCCGACAGTCAC
GAAATACTAGGAGAGCTAGCGTTTATGAGCTTCTATACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGAAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A809FNW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

100

100

1