Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   IRJ27_RS18670 Genome accession   NZ_CP064095
Coordinates   3615953..3616990 (+) Length   345 a.a.
NCBI ID   WP_009967727.1    Uniprot ID   A0AAE2SM96
Organism   Bacillus subtilis strain N1303-2Ay     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3610953..3621990
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IRJ27_RS18650 rsbRD 3611171..3612007 (+) 837 WP_004398754.1 RsbT co-antagonist protein RsbRD -
  IRJ27_RS18655 yqhB 3612060..3613388 (-) 1329 WP_009967730.1 hemolysin family protein -
  IRJ27_RS18660 corA 3613531..3614484 (+) 954 WP_004399136.1 magnesium transporter CorA -
  IRJ27_RS18665 comGA 3614896..3615966 (+) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  IRJ27_RS18670 comGB 3615953..3616990 (+) 1038 WP_009967727.1 comG operon protein ComGB Machinery gene
  IRJ27_RS18675 comGC 3617004..3617300 (+) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  IRJ27_RS18680 comGD 3617290..3617721 (+) 432 WP_004398628.1 comG operon protein ComGD Machinery gene
  IRJ27_RS18685 comGE 3617705..3618052 (+) 348 WP_003230165.1 ComG operon protein 5 Machinery gene
  IRJ27_RS18690 comGF 3618078..3618461 (+) 384 WP_003230168.1 ComG operon protein ComGF Machinery gene
  IRJ27_RS18695 comGG 3618462..3618836 (+) 375 WP_003230170.1 ComG operon protein ComGG Machinery gene
  IRJ27_RS18700 spoIIT 3618907..3619086 (+) 180 WP_003230176.1 YqzE family protein -
  IRJ27_RS18705 yqzG 3619128..3619454 (-) 327 WP_003246051.1 YqzG/YhdC family protein -
  IRJ27_RS18710 tapA 3619726..3620487 (+) 762 WP_004399106.1 amyloid fiber anchoring/assembly protein TapA -
  IRJ27_RS18715 sipW 3620471..3621043 (+) 573 WP_003246088.1 signal peptidase I -
  IRJ27_RS18720 tasA 3621107..3621892 (+) 786 WP_004398632.1 biofilm matrix protein TasA -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39762.26 Da        Isoelectric Point: 9.6257

>NTDB_id=499427 IRJ27_RS18670 WP_009967727.1 3615953..3616990(+) (comGB) [Bacillus subtilis strain N1303-2Ay]
MKKIRKVWLLKDQARLLKRLGEMTAGGYTLLDGLRLMELQMNKRQAADLTDSVTCLREGAPFYQVLKSLSFHKEAVGICY
FAETHGELPASMIQSGELLERKIAQADQLKRVLRYPLFLIFTVAVMFYMLQSIIIPQFSGIYQSMNMETSRSTDMLFAFF
QHIDLVIILLVLFTAGIGIYYWLVFKKKSPARQMLICIRIPLVGKLVKLFNSYFFSLQLSSLLKSGLSIYDSLNAFKHQT
FLPFYRCEAEQLIERLKAGESIESAICGSLFYETDLSKVISHGQLSGRLDRELFTYSQFILQRLEHKAQKWTGILQPMIY
GFVAAMILLVYLSMLVPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=499427 IRJ27_RS18670 WP_009967727.1 3615953..3616990(+) (comGB) [Bacillus subtilis strain N1303-2Ay]
ATGAAAAAGATTAGAAAAGTCTGGTTGTTAAAGGATCAAGCCAGGTTATTAAAGAGGCTCGGTGAAATGACTGCGGGCGG
ATATACACTTCTGGATGGATTACGCCTGATGGAACTTCAGATGAATAAGAGGCAGGCGGCTGACTTGACTGATTCGGTCA
CTTGTTTGAGGGAAGGGGCTCCGTTTTATCAAGTACTAAAGAGTTTGTCATTTCATAAGGAAGCCGTAGGTATTTGTTAT
TTTGCTGAAACACATGGTGAACTGCCTGCTTCAATGATCCAGAGCGGAGAGCTGCTGGAACGAAAAATTGCACAGGCAGA
CCAGCTGAAAAGAGTGCTGCGCTATCCGCTTTTCCTCATCTTTACGGTCGCTGTCATGTTTTATATGTTACAGTCCATCA
TCATTCCTCAGTTTTCCGGTATCTATCAATCGATGAATATGGAAACCTCACGTTCAACCGATATGCTTTTTGCTTTTTTT
CAGCATATTGATCTTGTGATCATTTTGCTTGTTCTTTTTACAGCAGGTATCGGGATTTATTATTGGCTTGTGTTTAAGAA
AAAATCACCTGCCCGGCAAATGCTGATTTGTATCAGGATTCCTTTGGTTGGAAAGCTTGTAAAGCTGTTTAACAGCTACT
TTTTTTCTTTGCAGCTAAGCAGCCTTTTAAAATCAGGCCTCTCAATTTATGACAGCCTTAATGCATTTAAACATCAAACG
TTTCTCCCTTTCTACCGCTGCGAGGCTGAACAATTGATTGAACGGCTAAAAGCCGGTGAGTCAATTGAATCCGCTATTTG
TGGAAGCCTTTTTTATGAAACTGATTTATCAAAAGTCATATCTCACGGCCAGCTGAGCGGCCGATTGGATCGGGAGCTTT
TCACATACAGCCAATTCATATTACAGCGGCTGGAACACAAAGCGCAAAAATGGACAGGCATCCTTCAGCCAATGATTTAT
GGATTTGTTGCAGCGATGATCTTACTTGTGTATTTATCTATGCTTGTGCCTATGTATCAGATGATGAATCAAATGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(17-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

100

93.623

0.936