Detailed information NTG0499116

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: IRJ22_RS01535
• Genome accession: NZ_CP064092
• Coordinates: 291891..295583 (+)
• Length: 1230 a.a.
• NCBI ID: WP_003180429.1
• Uniprot ID: -
• Organism: Bacillus licheniformis strain C5
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 286891..300583

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IRJ22_RS01525	modB	287638..288294 (+)	657	WP_003180426.1	molybdate ABC transporter permease subunit	-
IRJ22_RS01530	addB	288404..291904 (+)	3501	WP_003180427.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
IRJ22_RS01535	addA	291891..295583 (+)	3693	WP_003180429.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
IRJ22_RS01540	sbcD	295649..296830 (+)	1182	WP_003180431.1	exonuclease subunit SbcD	-
IRJ22_RS01545	sbcC	296827..300219 (+)	3393	WP_161658292.1	exonuclease subunit SbcC	-
IRJ22_RS01550	-	300264..300482 (-)	219	WP_016885923.1	spore germination protein	-

Sequence

Protein

• Length: 1230 a.a.
• Molecular weight: 141122.06 Da
• Isoelectric Point: 5.4667

>NTDB_id=499116 IRJ22_RS01535 WP_003180429.1 291891..295583(+) (addA) [Bacillus licheniformis strain C5]
MEISKPKGSNWTDDQWKAIVSSGRDILVAAAAGSGKTAVLVERIIRKITDQEHPVDVDRLLVVTFTNASAAEMKHRIGEA
LEKELAENPGSLHLRRQLALLNKASISTLHSFCLQVIRKYYYLIDVDPAFRIADQTEGELLGDEVLDELFEEEYKKGNPA
FFELVDRYTTDRHDLDLQHLVKRVYEFSRSHPDPEGWLHSLAELYDAASDTKVEALPFYSYIKEDIALVLEGMRQKLTRA
LDLTKQPGGPAPRAENFLDDLAQIDRLIQQQDDFSALHELVPTVSFQRLKPCKGDEYDPRLVDEAADLRNSAKKQLEKLK
SDYFSRTPEQHLESLREMKPVIQTLVQLVLEYGRRFAEAKKEKAIVDFSDLEHDCLAILSVKNPAGEAVPSEAAKFYRHQ
FHEVLVDEYQDTNLVQEAILKLVAKEEHEGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTDDGSGSGQKIDLNKNFRSR
SDILDSTNFLFKQLMGEKVGEVEYDEQAELKLGASYPPNEATKTELLLIETPSGAPGEEAEELEAVQLEARAMAGQIRRL
ITEKFQVYDAKAKASRNIQYRDIVVLLRSMPWAPQIMDEFKQQGIPVYANLSTGYFEATEVSVTLSLLKIIDNAYQDIPL
ASVLRSPVVGLDENELSLIRIKDKKAPFYEAMKAYLAAADGDERLSEKLRRFDGLLKKWRAYAKNHSVAELIWEIYRDTK
YLDYVGGMPGGKQRQANLRALYDRARSYEATSFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLEFP
VVFTAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPKWRISYPTLPLIAMKKKLRRELLSEELRVLYVALTRAKEKLYLVG
TAKDKEKLLADWRTQAAGSEWLLPDYERFQAKSYLDFIGPALMRHRDMDESGAPLVIDEIREHPARFQVSWLSAADLQAE
DIDQAGEERHGRLSQIQMGLPIDGSFEYEQQVRERLTWSYPYKDAAKVRTKQSVSEMKRQKEYEDEYGDRSLIRPTKDAL
LFKRPSFMMVKGLTASERGTAMHTVMQHIPLTRALEMNELSRLLDRLVEKELLTEDQRAAIEEDDILAFFDTEIGQKLLG
ARRVEREVPFNMTLSAKEVYPDLESADEPVLIQGIIDCLFETEDGFYLLDYKTDRIHGKYRNGFEGAEPILRKRYETQIQ
LYARAVETMIKMPLKGRALYFFDGGHVLLF

Nucleotide

• Length: 3693 bp

>NTDB_id=499116 IRJ22_RS01535 WP_003180429.1 291891..295583(+) (addA) [Bacillus licheniformis strain C5]
ATGGAGATTTCTAAACCGAAGGGCAGCAATTGGACTGATGACCAATGGAAGGCGATTGTCTCATCCGGCCGGGACATTTT
AGTTGCGGCAGCGGCCGGCTCCGGAAAAACGGCCGTGCTGGTTGAGCGGATCATTCGGAAAATAACGGATCAGGAACATC
CCGTAGATGTCGACAGGCTGCTTGTCGTCACCTTTACAAATGCATCTGCGGCTGAAATGAAGCACAGGATCGGCGAAGCG
CTCGAAAAAGAGCTTGCCGAAAATCCGGGATCGCTTCATTTGCGCCGACAGCTTGCTTTATTAAACAAAGCGAGCATATC
AACATTGCATTCATTCTGTCTTCAAGTCATCCGCAAATATTACTATTTGATCGATGTCGATCCGGCTTTCCGGATCGCCG
ATCAGACGGAAGGCGAGCTGCTCGGCGATGAGGTGCTGGATGAACTGTTTGAAGAAGAATACAAAAAAGGCAATCCTGCC
TTTTTTGAACTTGTAGACAGATATACGACAGACCGTCATGACCTCGACCTGCAGCATCTTGTCAAGCGGGTCTACGAATT
TTCCCGTTCGCATCCCGATCCGGAGGGCTGGCTTCACTCATTGGCTGAACTGTATGACGCGGCTTCGGATACGAAGGTTG
AGGCTCTGCCGTTTTATTCTTACATTAAAGAAGACATCGCACTCGTTCTGGAAGGTATGCGGCAAAAGCTGACGCGCGCG
CTTGATTTGACGAAGCAGCCCGGGGGGCCTGCTCCGCGCGCCGAAAATTTTCTCGATGATTTAGCGCAGATCGACAGGCT
GATTCAGCAGCAGGATGATTTTTCGGCCCTGCATGAGCTTGTACCGACCGTTTCGTTTCAACGATTGAAGCCATGCAAAG
GTGATGAGTACGATCCCAGATTGGTTGATGAGGCGGCAGACCTCCGCAACAGTGCGAAAAAGCAGCTTGAAAAGCTGAAA
AGCGATTATTTTTCGCGAACTCCGGAGCAGCACCTTGAGAGCCTGAGGGAAATGAAGCCTGTCATTCAGACGCTTGTCCA
GCTCGTGCTGGAGTATGGCAGGCGGTTTGCGGAAGCGAAAAAGGAAAAGGCGATCGTCGATTTTTCAGACCTTGAACACG
ACTGCCTTGCGATTTTATCCGTGAAAAATCCGGCGGGCGAGGCCGTGCCGAGCGAAGCGGCGAAGTTTTACCGCCATCAG
TTCCACGAAGTGCTTGTTGATGAATACCAGGATACGAACCTGGTCCAGGAAGCGATTTTAAAGCTCGTCGCCAAAGAGGA
GCATGAGGGGAACCTGTTTATGGTGGGCGATGTCAAGCAGTCGATTTACCGGTTCCGCCTGGCTGAGCCGCTTTTGTTCC
TCTCGAAATATAAACGCTTTACAGACGATGGCTCGGGTTCCGGCCAGAAAATCGATTTAAATAAAAACTTCAGAAGCCGT
TCAGATATTTTGGACAGCACGAACTTTTTATTCAAACAGCTCATGGGAGAAAAAGTCGGGGAAGTCGAGTATGACGAACA
AGCCGAATTGAAGCTCGGCGCCTCTTATCCGCCGAACGAAGCGACAAAAACGGAGCTTCTGCTCATCGAAACCCCGAGCG
GCGCGCCGGGTGAAGAGGCTGAAGAGCTTGAGGCGGTCCAGCTTGAAGCAAGAGCGATGGCCGGGCAAATCCGCAGGCTG
ATTACAGAAAAATTTCAAGTATATGATGCCAAAGCGAAAGCGTCGCGAAACATCCAGTACCGTGATATCGTGGTGCTTTT
GCGGTCGATGCCTTGGGCGCCTCAAATTATGGACGAGTTTAAACAGCAGGGAATACCCGTATACGCGAACCTCTCAACTG
GTTATTTTGAGGCGACAGAAGTATCGGTCACACTGTCTTTATTAAAAATCATCGATAATGCCTATCAGGACATCCCGCTT
GCGTCCGTTCTCAGATCGCCTGTTGTGGGTCTTGATGAGAATGAGCTTTCCTTGATCAGAATCAAAGATAAAAAAGCGCC
GTTTTACGAAGCCATGAAAGCGTACCTTGCTGCAGCAGACGGCGATGAGAGGCTCAGCGAAAAGCTTCGCCGCTTTGACG
GTCTGCTCAAGAAGTGGAGAGCTTATGCGAAAAACCACTCTGTAGCAGAGCTGATCTGGGAAATCTACCGGGATACGAAG
TATTTGGATTATGTCGGCGGAATGCCGGGCGGAAAACAGCGGCAGGCCAATTTGCGCGCGCTTTACGACAGGGCGAGATC
CTATGAAGCGACATCATTCCGCGGCTTGTTCCGCTTTTTAAGATTTATTGAGCGGATGCAGGAGCGGGGCGATGACCTCG
GAACGGCAAGGGCTCTAAGCGAACAGGAAGACGTTGTTCGGCTGATGACGATCCACTCAAGCAAAGGGCTTGAATTTCCT
GTCGTATTTACCGCCGGTCTCGGACGGAATTTTAATATGATGGACTTGAACAAATCTTATCTGCTGGATAAAGAGCTCGG
TTTTGGAACGAAGTTTATCCATCCGAAGTGGCGGATCAGCTATCCGACGCTGCCGCTTATCGCGATGAAGAAAAAACTCC
GCCGCGAGCTTTTATCAGAGGAGCTGCGCGTCTTGTATGTCGCCCTTACCCGGGCCAAGGAAAAGCTGTACCTGGTCGGG
ACGGCGAAGGATAAAGAAAAGCTGCTTGCCGACTGGCGGACACAAGCGGCGGGGTCTGAGTGGCTGCTTCCGGATTATGA
ACGGTTTCAGGCGAAATCTTACCTGGATTTCATCGGACCTGCTTTGATGAGACACAGAGATATGGATGAATCCGGCGCGC
CGCTGGTGATTGATGAGATCCGTGAACATCCGGCGCGCTTTCAGGTCAGCTGGCTGTCGGCCGCGGATCTTCAAGCAGAA
GATATTGATCAGGCCGGTGAAGAAAGACACGGCCGGCTCTCCCAAATTCAAATGGGGCTTCCGATCGATGGTTCGTTTGA
GTATGAGCAACAAGTCAGAGAAAGGCTGACATGGTCATATCCATACAAAGACGCCGCCAAAGTCCGAACAAAACAGTCTG
TCTCAGAAATGAAAAGGCAGAAGGAGTATGAGGATGAGTACGGCGACCGCTCGCTGATTCGCCCAACCAAGGATGCTCTC
CTGTTTAAACGGCCGTCCTTTATGATGGTTAAAGGCCTGACCGCTTCCGAACGGGGAACGGCGATGCACACCGTCATGCA
GCATATTCCGCTGACCCGTGCGCTTGAAATGAATGAGCTCAGCCGCCTCCTAGACAGGCTTGTTGAGAAAGAGCTATTAA
CAGAAGACCAGCGGGCAGCGATTGAGGAAGACGATATTCTCGCATTCTTTGATACGGAAATCGGACAAAAGCTTCTCGGC
GCCCGCCGCGTCGAACGGGAAGTTCCCTTTAACATGACGCTGTCCGCCAAAGAGGTTTATCCTGATCTTGAGTCAGCCGA
CGAACCTGTATTGATACAGGGAATTATCGATTGTTTATTCGAGACGGAGGACGGTTTTTACCTTCTTGATTACAAAACCG
ACCGGATACACGGAAAGTACCGAAACGGATTCGAAGGGGCCGAACCGATTTTAAGGAAGCGTTACGAAACACAAATTCAG
CTTTATGCACGAGCCGTTGAAACGATGATTAAAATGCCGCTCAAAGGGCGTGCACTGTACTTTTTTGACGGAGGACATGT
GCTCTTATTTTAA

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (471-880)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-460)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1053-1222)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	71.893	100	0.72