Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ISX45_RS16185 Genome accession   NZ_CP064060
Coordinates   3236553..3237932 (+) Length   459 a.a.
NCBI ID   WP_181557458.1    Uniprot ID   A0A7W0C164
Organism   Thermaerobacillus caldiproteolyticus strain U458     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3231553..3242932
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ISX45_RS16165 (ISX45_16165) - 3231919..3232380 (+) 462 WP_181557461.1 CtsR family transcriptional regulator -
  ISX45_RS16170 (ISX45_16170) - 3232397..3232945 (+) 549 WP_194519412.1 UvrB/UvrC motif-containing protein -
  ISX45_RS16175 (ISX45_16175) - 3232950..3234038 (+) 1089 WP_181557459.1 protein arginine kinase -
  ISX45_RS16180 (ISX45_16180) clpC 3234038..3236470 (+) 2433 WP_181557462.1 ATP-dependent protease ATP-binding subunit ClpC -
  ISX45_RS16185 (ISX45_16185) radA 3236553..3237932 (+) 1380 WP_181557458.1 DNA repair protein RadA Machinery gene
  ISX45_RS16190 (ISX45_16190) - 3238046..3239140 (+) 1095 WP_181557457.1 PIN/TRAM domain-containing protein -
  ISX45_RS16195 (ISX45_16195) ispD 3239164..3239850 (+) 687 WP_181557456.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  ISX45_RS16200 (ISX45_16200) ispF 3239868..3240344 (+) 477 WP_181557455.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  ISX45_RS16205 (ISX45_16205) gltX 3240418..3241878 (+) 1461 WP_181557454.1 glutamate--tRNA ligase -
  ISX45_RS16210 (ISX45_16210) cysE 3242217..3242885 (+) 669 WP_181557453.1 serine O-acetyltransferase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50191.81 Da        Isoelectric Point: 7.1860

>NTDB_id=498915 ISX45_RS16185 WP_181557458.1 3236553..3237932(+) (radA) [Thermaerobacillus caldiproteolyticus strain U458]
MGKKKTKFVCQECGYESVKWMGRCPGCYTWNSMVEEVERAKPTTRGVFVHSSPDTISKPVPITTVTTTQEPRIQTDLSEL
NRVLGGGIVRGSLVLIGGDPGIGKSTLLLQVSAQLAMKQNKVLYISGEESVKQTKLRADRLHVSSNELYVLAETDLEYIA
EAIEVINPAFVVIDSIQTVYRSEITSAPGSVSQVRECTAELMKIAKTKGIAIFIVGHVTKEGAIAGPRILEHMVDTVLYF
EGERHHTYRILRAVKNRFGSTNEIGIFEMRETGLLEVENPSEVFLEERSRGAAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFRDQPTNPYDVIIGEVGLT
GEVRRVSRIEQRVQEAVKLGFHRIIIPKNNVGGWNVPAGVEIIGVSHVAEALEYTLGSK

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=498915 ISX45_RS16185 WP_181557458.1 3236553..3237932(+) (radA) [Thermaerobacillus caldiproteolyticus strain U458]
ATGGGAAAGAAAAAAACAAAGTTTGTTTGTCAAGAATGCGGGTACGAGTCGGTCAAATGGATGGGAAGATGCCCGGGATG
TTATACATGGAATTCTATGGTAGAGGAAGTAGAGCGAGCAAAACCAACAACGAGGGGAGTGTTCGTTCATTCGTCTCCCG
ATACAATTTCTAAGCCGGTACCGATTACCACAGTGACGACAACACAAGAGCCGCGCATACAAACAGATCTGTCTGAATTG
AACCGCGTGTTAGGCGGGGGCATTGTCAGAGGCTCTCTTGTCTTAATTGGCGGTGATCCAGGGATCGGAAAATCAACGCT
TCTTTTGCAAGTTTCGGCACAACTCGCGATGAAACAAAATAAAGTGTTATATATATCTGGTGAAGAATCGGTCAAACAAA
CGAAATTACGGGCAGATCGCCTTCATGTTTCATCTAATGAATTGTATGTGTTAGCAGAGACGGATTTAGAATATATTGCG
GAGGCGATTGAGGTAATCAATCCTGCCTTTGTCGTTATTGATTCAATTCAAACGGTGTATCGTTCGGAAATTACGTCAGC
TCCCGGAAGTGTGTCCCAAGTGCGCGAATGCACAGCAGAGCTAATGAAAATCGCGAAGACAAAAGGGATTGCCATTTTTA
TCGTCGGTCATGTGACAAAAGAGGGGGCAATTGCCGGACCGAGAATTTTAGAGCATATGGTTGATACCGTGCTTTATTTT
GAAGGGGAACGTCATCATACGTACCGCATTTTACGAGCGGTAAAAAACCGCTTTGGTTCCACCAATGAAATTGGCATTTT
TGAGATGAGAGAAACCGGTTTATTGGAAGTGGAGAATCCGTCCGAAGTATTTTTAGAGGAGCGTTCACGAGGAGCGGCAG
GGTCGACAGTTGTTGCCTCTATGGAAGGAACCCGGCCTGTGTTAGTAGAAATTCAAGCATTAATTTCACCGACGAGTTTT
GGAAATCCGCGGAGAATGGCGACGGGAATAGACCATAACCGCGTCTCTTTGTTAATGGCAGTACTTGAAAAACGCGTCGG
GCTACTTCTCCAAAACCAAGATGCTTACTTAAAAGTGGCAGGCGGCGTCAAATTGGATGAACCGGCGATTGATTTAGCGA
TTGCTGTAAGCATTGCCTCCAGTTTTCGTGATCAGCCTACGAATCCTTATGATGTTATTATCGGTGAAGTCGGCTTAACA
GGGGAGGTCCGCCGCGTTTCCCGCATTGAACAACGCGTACAGGAAGCAGTAAAATTAGGATTTCATCGTATTATTATTCC
AAAAAACAATGTAGGCGGATGGAATGTACCAGCAGGAGTAGAAATCATCGGCGTGTCTCACGTTGCCGAAGCGCTTGAAT
ACACATTAGGAAGCAAGTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)

AAA domain (AAA_25)

(68-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7W0C164

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

77.462

99.564

0.771
  radA Streptococcus pneumoniae Rx1

63.576

98.693

0.627
  radA Streptococcus pneumoniae D39

63.576

98.693

0.627
  radA Streptococcus pneumoniae R6

63.576

98.693

0.627
  radA Streptococcus pneumoniae TIGR4

63.576

98.693

0.627
  radA Streptococcus mitis SK321

63.576

98.693

0.627
  radA Streptococcus mitis NCTC 12261

63.576

98.693

0.627