Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   DHf2319_RS02400 Genome accession   NZ_CP063982
Coordinates   495709..496779 (+) Length   356 a.a.
NCBI ID   WP_243479211.1    Uniprot ID   -
Organism   Orrella daihaiensis strain f23     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 490709..501779
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DHf2319_RS02370 (DHf2319_02370) - 492040..492888 (+) 849 WP_243479205.1 class I SAM-dependent methyltransferase -
  DHf2319_RS02375 (DHf2319_02375) - 492907..493917 (+) 1011 WP_243479206.1 AsmA family protein -
  DHf2319_RS02380 (DHf2319_02380) - 493963..494346 (+) 384 WP_243479207.1 MOSC N-terminal beta barrel domain-containing protein -
  DHf2319_RS02385 (DHf2319_02385) - 494350..494613 (-) 264 WP_243479208.1 YfhL family 4Fe-4S dicluster ferredoxin -
  DHf2319_RS02390 (DHf2319_02390) coaD 494632..495150 (-) 519 WP_243479209.1 pantetheine-phosphate adenylyltransferase -
  DHf2319_RS02395 (DHf2319_02395) rsmD 495147..495707 (-) 561 WP_243479210.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  DHf2319_RS02400 (DHf2319_02400) pilA 495709..496779 (+) 1071 WP_243479211.1 signal recognition particle-docking protein FtsY Machinery gene
  DHf2319_RS02405 (DHf2319_02405) - 496776..498341 (-) 1566 WP_243479212.1 CYTH and CHAD domain-containing protein -
  DHf2319_RS02410 (DHf2319_02410) ribBA 498507..499703 (+) 1197 WP_243479213.1 bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II -
  DHf2319_RS02415 (DHf2319_02415) ribH 499700..500194 (+) 495 WP_243479214.1 6,7-dimethyl-8-ribityllumazine synthase -
  DHf2319_RS02420 (DHf2319_02420) nusB 500206..500700 (+) 495 WP_243479215.1 transcription antitermination factor NusB -
  DHf2319_RS02425 (DHf2319_02425) thiL 500712..501698 (+) 987 WP_243479216.1 thiamine-phosphate kinase -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 37988.41 Da        Isoelectric Point: 6.4178

>NTDB_id=498283 DHf2319_RS02400 WP_243479211.1 495709..496779(+) (pilA) [Orrella daihaiensis strain f23]
MFRFFRKKDRPQSSDAQTESVSDIIVDSPSLPAIEPQQAPPAPQALAQPVPESASQSWMSRLRQGLARTSIGGIFTGSKV
DESLYDELESALIMSDAGVQASTTLIEALRQTVRKERLQSAQEVREALTDVLTEHLRALEKPFEVGAIKPLVVMIAGVNG
AGKTTSIGKLAHALQAGGARVLLAAGDTFRAAAREQLIEWGNRNHVAVIAQEGGDPAAVAFDAVTAGHARHMDVVMVDTA
GRLPTQLHLMEELKKIRRVIHKADQTAPHEVLLVVDGNTGQNALAQVKAFDTALNLTGLIVTKLDGTAKGGVLAALASGA
QGCRPIPVYWIGVGEQLEDLQPFVAREFAQAMLGTH

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=498283 DHf2319_RS02400 WP_243479211.1 495709..496779(+) (pilA) [Orrella daihaiensis strain f23]
ATGTTCAGATTCTTCCGTAAAAAAGACCGCCCACAGTCATCTGATGCTCAGACAGAGTCAGTCAGTGACATTATCGTTGA
TTCGCCTAGTTTGCCTGCTATCGAGCCGCAACAGGCTCCCCCCGCACCCCAAGCGTTAGCACAGCCCGTGCCAGAGTCGG
CCAGCCAATCATGGATGAGTCGTCTGCGCCAGGGGTTGGCCAGAACCAGTATCGGTGGGATTTTTACAGGCAGCAAAGTC
GATGAAAGCCTGTATGACGAGCTAGAGTCCGCACTCATTATGAGTGATGCAGGGGTGCAGGCCAGCACGACCTTGATAGA
GGCACTTAGACAAACGGTTCGTAAAGAGCGTCTTCAGAGTGCGCAAGAGGTGCGCGAGGCACTGACCGATGTGCTCACGG
AGCATTTGCGTGCATTGGAAAAGCCGTTTGAAGTGGGTGCGATCAAGCCTTTGGTGGTCATGATTGCAGGTGTCAACGGT
GCCGGTAAGACAACCTCGATTGGCAAACTTGCACACGCTTTGCAGGCAGGCGGTGCGCGGGTGTTGTTGGCAGCCGGGGA
TACGTTTAGGGCGGCCGCCCGAGAGCAATTGATAGAGTGGGGTAACCGCAACCATGTGGCCGTGATCGCGCAAGAGGGGG
GCGACCCTGCTGCAGTGGCATTTGATGCGGTTACAGCCGGTCACGCTCGTCACATGGATGTGGTGATGGTGGATACTGCC
GGTAGACTGCCAACACAGCTGCATTTAATGGAAGAGCTCAAGAAGATTCGAAGGGTGATTCACAAAGCTGATCAAACGGC
TCCACACGAAGTACTGCTTGTGGTTGACGGCAACACTGGGCAAAACGCCTTGGCGCAGGTCAAGGCATTTGATACTGCGT
TAAATCTGACCGGTCTGATTGTGACCAAGCTCGATGGCACCGCCAAAGGTGGTGTGCTGGCGGCGCTAGCCTCGGGTGCT
CAAGGTTGTCGTCCGATTCCGGTCTACTGGATCGGTGTGGGCGAGCAACTCGAGGACCTGCAGCCATTTGTGGCCCGAGA
GTTTGCTCAGGCCATGCTAGGCACTCACTAA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(71-132)

SRP54-type protein, GTPase domain (SRP54)

(151-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

52.394

99.719

0.522