Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   ISN59_RS05235 Genome accession   NZ_CP063980
Coordinates   1098885..1100696 (+) Length   603 a.a.
NCBI ID   WP_016622211.1    Uniprot ID   A0A8B3RWY7
Organism   Enterococcus faecalis strain AR_0780     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1093885..1105696
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ISN59_RS05215 (ISN59_05200) trpS 1094795..1095805 (-) 1011 WP_002378619.1 tryptophan--tRNA ligase -
  ISN59_RS05220 (ISN59_05205) spxA 1096166..1096564 (+) 399 WP_002356477.1 transcriptional regulator SpxA -
  ISN59_RS05225 (ISN59_05210) - 1096907..1097569 (+) 663 WP_002356478.1 adaptor protein MecA -
  ISN59_RS05230 (ISN59_05215) - 1097659..1098837 (+) 1179 WP_002387170.1 competence protein CoiA -
  ISN59_RS05235 (ISN59_05220) pepF 1098885..1100696 (+) 1812 WP_016622211.1 oligoendopeptidase F Regulator
  ISN59_RS05240 (ISN59_05225) - 1100788..1101447 (-) 660 WP_002368840.1 ClpXP adapter SpxH family protein -
  ISN59_RS05245 (ISN59_05230) - 1101511..1102089 (-) 579 WP_002356483.1 CYTH domain-containing protein -
  ISN59_RS05250 (ISN59_05235) - 1102286..1102960 (+) 675 WP_002356484.1 GTP pyrophosphokinase family protein -
  ISN59_RS05255 (ISN59_05240) - 1102973..1103770 (+) 798 WP_002356486.1 NAD kinase -
  ISN59_RS05260 (ISN59_05245) - 1103778..1104671 (+) 894 WP_002356487.1 RluA family pseudouridine synthase -

Sequence

Protein


Download         Length: 603 a.a.        Molecular weight: 68847.18 Da        Isoelectric Point: 4.7145

>NTDB_id=498262 ISN59_RS05235 WP_016622211.1 1098885..1100696(+) (pepF) [Enterococcus faecalis strain AR_0780]
MSEIKQLPTRDEVPTPLTWDLTKIFKDDAAFDVAYNQLIEELNQAESFKGTLGNGAEAFLAALEYVLDVYRKVETLYVYS
HLKNDQDTTNTAYQALYARASSLYAQVSEAVSWFDPEVLTLSDEQIWGYFEEQPKLAVYRHYIQNILDERPHVLSMEQEA
LLAGASEIFGASSNTFSILNNADLEFPTVQNAEGETIQLSHGVYGQLMESVDPSVREAAFKGLYKVYKQFRNTLASTLGA
HVKTHNYKAKIRNYDSARAASLASNHIPESVHETLVAVVNKHLPLLHRYVKLRKKLLNVEELHMYDLYAPLLGEAPIRYS
YEEAKEKAIEALKPLGEDYLSIVKEAFSSRWIDVIENQGKRSGAYSSGAYDTAPYILMNWHDSLDQLFTLVHEMGHSVHS
YYTRNNQPYVYGDYSIFLAEIASTTNENILTEYLLQTETDPKVRAYVLNHYLDGFKGTIFRQTQFAEFEHFIHTEDAKGT
PLTSEYLSEYYGELNAKYYGPEVVRDEEISYEWARIPHFYYNYYVYQYATGFSAASALSKHILAGEEGALENYLNYLKAG
SSDFPIEVMKKAGVDMTQAAYIEDAMKVFEERLTELEALVEKL

Nucleotide


Download         Length: 1812 bp        

>NTDB_id=498262 ISN59_RS05235 WP_016622211.1 1098885..1100696(+) (pepF) [Enterococcus faecalis strain AR_0780]
ATGAGCGAAATTAAACAATTACCAACACGTGATGAAGTTCCAACTCCATTGACTTGGGATTTAACCAAAATTTTTAAAGA
TGATGCTGCTTTTGATGTTGCGTATAATCAGTTGATAGAAGAGCTAAATCAAGCAGAATCATTTAAAGGTACCTTAGGGA
ATGGTGCAGAAGCTTTTCTAGCTGCGTTAGAATACGTTTTAGATGTCTATCGAAAAGTGGAAACATTATATGTCTATTCT
CATTTAAAAAATGATCAAGACACTACAAATACAGCTTACCAAGCTTTATATGCCAGAGCTAGTTCTTTATATGCACAAGT
TAGTGAAGCTGTTTCTTGGTTTGACCCAGAGGTTTTAACATTAAGTGATGAACAAATCTGGGGATATTTTGAAGAGCAGC
CGAAATTAGCTGTTTATCGTCACTATATTCAAAATATTTTAGATGAACGTCCCCATGTTTTATCGATGGAACAAGAAGCT
TTACTGGCGGGGGCTAGTGAAATTTTTGGTGCCTCAAGTAATACATTTTCAATTTTAAATAATGCAGACTTAGAATTTCC
AACTGTGCAAAATGCTGAAGGCGAAACGATTCAACTTTCTCATGGCGTTTATGGTCAGTTAATGGAAAGTGTCGATCCAT
CTGTTCGTGAAGCAGCATTTAAAGGTTTGTACAAAGTATACAAACAATTTAGAAATACATTAGCCTCAACTTTAGGTGCA
CATGTTAAAACACATAATTATAAAGCAAAAATTAGAAATTATGATTCTGCTCGAGCAGCCTCTTTAGCAAGTAATCATAT
CCCTGAAAGTGTTCACGAAACATTAGTAGCTGTAGTAAATAAACATTTACCTTTGCTACATCGTTATGTAAAATTAAGAA
AAAAATTATTAAACGTAGAAGAATTACACATGTACGACTTGTATGCGCCTTTGCTAGGTGAAGCACCAATTCGTTACAGC
TATGAAGAAGCAAAAGAAAAAGCAATTGAGGCTTTAAAACCACTAGGTGAAGACTATTTATCTATTGTTAAAGAAGCTTT
TTCAAGTCGCTGGATTGATGTGATTGAAAATCAAGGAAAACGAAGCGGCGCATATTCTTCAGGAGCTTATGATACAGCCC
CATACATTTTAATGAATTGGCATGATAGTTTGGATCAACTATTTACATTAGTCCATGAGATGGGCCATAGTGTTCATAGT
TACTATACAAGAAATAATCAGCCGTATGTTTATGGCGACTATTCAATTTTCTTAGCTGAGATTGCTTCAACCACAAATGA
AAATATTTTAACAGAATATTTATTACAAACAGAAACAGATCCTAAAGTACGTGCGTATGTCTTAAATCACTATTTAGACG
GCTTTAAGGGAACCATTTTCCGTCAAACGCAATTTGCGGAATTTGAACATTTTATTCACACGGAAGATGCTAAAGGCACG
CCATTAACAAGTGAATATTTGAGTGAGTATTATGGCGAGCTCAATGCTAAATATTATGGACCAGAAGTAGTTAGAGACGA
AGAAATCAGTTACGAATGGGCGAGAATTCCACATTTTTATTACAATTACTATGTTTATCAATATGCAACTGGCTTCTCAG
CCGCTTCGGCATTGTCTAAACATATTTTAGCTGGAGAAGAGGGAGCTTTAGAGAATTATCTTAACTACTTGAAAGCAGGA
AGTAGTGACTTCCCAATTGAAGTGATGAAAAAAGCGGGCGTGGATATGACACAAGCCGCGTATATCGAAGATGCAATGAA
AGTTTTTGAAGAACGTTTAACGGAATTAGAAGCTTTGGTTGAAAAATTATAG

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(207-587)

Oligopeptidase F (Peptidase_M3_N)

(117-186)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A8B3RWY7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

54.637

98.342

0.537