Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   IP614_RS27290 Genome accession   NZ_CP063975
Coordinates   5360180..5361223 (-) Length   347 a.a.
NCBI ID   WP_074626608.1    Uniprot ID   -
Organism   Bacillus cereus strain N435-1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5355180..5366223
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IP614_RS27245 - 5355611..5355811 (-) 201 WP_000106083.1 YqzE family protein -
  IP614_RS27250 aroK 5355850..5356347 (-) 498 WP_074626601.1 shikimate kinase AroK -
  IP614_RS27255 - 5356463..5357113 (-) 651 WP_000183860.1 2OG-Fe(II) oxygenase -
  IP614_RS27260 comGG 5357290..5357661 (-) 372 WP_001231273.1 competence type IV pilus minor pilin ComGG -
  IP614_RS27265 comGF 5357658..5358128 (-) 471 WP_000923099.1 competence type IV pilus minor pilin ComGF -
  IP614_RS27270 - 5358098..5358400 (-) 303 WP_000829473.1 hypothetical protein -
  IP614_RS27275 comGD 5358393..5358848 (-) 456 WP_000813142.1 competence type IV pilus minor pilin ComGD -
  IP614_RS27280 comGC 5358845..5359144 (-) 300 WP_001178686.1 competence type IV pilus major pilin ComGC -
  IP614_RS27285 comGB 5359156..5360187 (-) 1032 WP_074626606.1 competence type IV pilus assembly protein ComGB -
  IP614_RS27290 comGA 5360180..5361223 (-) 1044 WP_074626608.1 competence type IV pilus ATPase ComGA Machinery gene
  IP614_RS27295 - 5361429..5362124 (+) 696 WP_000434125.1 helix-turn-helix domain-containing protein -
  IP614_RS27300 - 5362250..5362492 (+) 243 WP_000440721.1 DUF2626 domain-containing protein -
  IP614_RS27305 - 5362599..5363993 (+) 1395 WP_074625302.1 L-cystine transporter -
  IP614_RS27310 - 5364073..5364466 (-) 394 Protein_5345 hypothetical protein -
  IP614_RS27315 - 5364554..5364769 (-) 216 WP_001008326.1 DUF3912 family protein -
  IP614_RS27320 - 5365022..5365333 (+) 312 WP_001093237.1 hypothetical protein -
  IP614_RS27325 - 5365368..5365856 (-) 489 WP_000764267.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39127.50 Da        Isoelectric Point: 8.5482

>NTDB_id=498244 IP614_RS27290 WP_074626608.1 5360180..5361223(-) (comGA) [Bacillus cereus strain N435-1]
MNGIEIFANAILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTRQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGHELKQALKEADGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=498244 IP614_RS27290 WP_074626608.1 5360180..5361223(-) (comGA) [Bacillus cereus strain N435-1]
ATGAATGGGATTGAAATTTTTGCGAATGCAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCAGTGCAACTACGTATAGGAAAAGATTTAATGACGAGACAGTGTATTGAAAAGGAGTTTGGAG
AAAAACTCGTCTCACATTTCAAATTTTTAGCATCTATGGACATAGGAGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTCGTGTTTACTGGACCGACTGGATCTGGGAAGACGACGACAATGTATGCATTATTAGAAGTA
ATTAGAAAAAAGAAAACACGTCGCATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGGGATGAGGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTAATGACAACGCTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGTTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTGGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAAGCAAGCATTTATGAGCTACTATATGGACATGAGTTAAAACAAGCACTTAAAGAAGCAGATGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATACGAAAAGGATATGCTTTAGGTTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.908

100

0.559