Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   IG390_RS12345 Genome accession   NZ_CP063965
Coordinates   2630220..2631581 (-) Length   453 a.a.
NCBI ID   WP_039258101.1    Uniprot ID   -
Organism   Clostridium botulinum strain BKT2873     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2625220..2636581
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IG390_RS12325 (IG390_12285) ispD 2626598..2627284 (-) 687 WP_039258961.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  IG390_RS12330 (IG390_12290) - 2627305..2628384 (-) 1080 WP_039258103.1 PIN domain-containing protein -
  IG390_RS12335 (IG390_12295) - 2628687..2629088 (+) 402 WP_013724431.1 hypothetical protein -
  IG390_RS12340 (IG390_12300) disA 2629125..2630189 (-) 1065 WP_039258102.1 DNA integrity scanning diadenylate cyclase DisA -
  IG390_RS12345 (IG390_12305) radA 2630220..2631581 (-) 1362 WP_039258101.1 DNA repair protein RadA Machinery gene
  IG390_RS12350 (IG390_12310) - 2631745..2634186 (-) 2442 WP_039258100.1 ATP-dependent Clp protease ATP-binding subunit -
  IG390_RS12355 (IG390_12315) - 2634211..2635245 (-) 1035 WP_039277130.1 protein arginine kinase -
  IG390_RS12360 (IG390_12320) - 2635264..2635782 (-) 519 WP_039258098.1 UvrB/UvrC motif-containing protein -
  IG390_RS12365 (IG390_12325) - 2635852..2636310 (-) 459 WP_039258097.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 50029.11 Da        Isoelectric Point: 7.8756

>NTDB_id=498193 IG390_RS12345 WP_039258101.1 2630220..2631581(-) (radA) [Clostridium botulinum strain BKT2873]
MAKSKNVFICQECGYEAPKWFGKCPGCATWNSMVEEKKMDTIKSKRVGITQNNSKPESILNIKSGEFNRYDTGIVELNRV
LGGGLVKGSLTLISGAPGIGKSTILLQTANNIASKVGKVLYVSGEESGEQIKIRGDRIGNISSELYILSETNLELIEEHI
NFMEPVFVIIDSIQTLFKPSIESAPGSVSQVRECSNELMRIAKTKNIPLFIVAHVTKQGELAGPRVLEHMVDTVLSFEGE
RTQEFRILRTLKNRFGTTSEIGVFEMRQEGLKEIFNPSEVFLEETNFNSEGSIVIGIMEGTRPILVEIQALVTETKAIMP
RRTAVGVDNSRLNLILAVLEKKLKIPFYNCDVYVNVVGGMDIEGTYGDLGLALALISSIKGRNFKLDKMIVFGEIGLTGE
VRPISLCDRLVNEGIKMGFENIVIPTRNKEKIVTKNKNIIGVSSLREAVSKVF

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=498193 IG390_RS12345 WP_039258101.1 2630220..2631581(-) (radA) [Clostridium botulinum strain BKT2873]
ATGGCTAAGAGCAAAAATGTTTTTATATGCCAAGAATGTGGGTACGAGGCTCCTAAATGGTTTGGAAAATGTCCAGGTTG
TGCTACATGGAATAGTATGGTTGAAGAAAAAAAAATGGATACTATAAAGAGCAAAAGAGTTGGCATAACTCAAAACAATA
GTAAGCCTGAGAGTATATTAAATATTAAATCGGGAGAATTTAATAGATATGATACTGGTATTGTAGAATTAAATAGAGTA
CTTGGTGGAGGGCTTGTTAAAGGATCTTTAACATTAATATCAGGGGCTCCTGGTATAGGGAAATCTACAATATTACTACA
AACAGCTAATAATATTGCATCTAAAGTAGGTAAAGTATTATATGTATCAGGTGAAGAATCAGGAGAACAAATAAAAATTA
GAGGAGATAGAATAGGAAATATATCCTCAGAATTATACATTTTATCTGAAACTAATTTGGAATTAATAGAAGAACATATT
AATTTTATGGAACCTGTGTTTGTTATTATAGATTCTATACAGACATTATTTAAACCTTCTATAGAATCAGCACCAGGTAG
TGTATCTCAAGTTAGAGAATGTTCTAATGAGCTTATGAGAATTGCAAAGACAAAAAATATACCGTTATTTATAGTTGCTC
ATGTAACTAAACAAGGGGAACTTGCAGGCCCTAGGGTATTGGAGCATATGGTAGATACGGTTCTTTCTTTTGAGGGTGAA
AGAACACAAGAGTTTAGAATATTAAGAACATTAAAAAATAGATTTGGTACTACAAGTGAAATTGGAGTATTTGAAATGAG
ACAAGAAGGGCTTAAGGAAATCTTCAATCCATCCGAAGTATTTCTAGAGGAGACTAATTTTAATTCAGAAGGGTCTATAG
TAATAGGTATTATGGAAGGAACTCGACCTATTTTGGTCGAAATACAAGCTCTAGTTACAGAAACTAAAGCAATTATGCCA
AGAAGAACAGCGGTAGGCGTTGATAATTCTAGGTTAAACTTGATATTAGCAGTACTAGAGAAAAAATTAAAAATACCTTT
TTACAACTGTGATGTTTATGTCAATGTAGTTGGTGGCATGGATATTGAAGGGACATATGGAGACTTAGGTCTTGCTCTAG
CTTTGATATCAAGTATTAAGGGAAGAAATTTTAAACTTGATAAAATGATAGTTTTTGGGGAAATAGGTTTAACAGGAGAA
GTAAGACCTATTTCATTATGTGATAGACTTGTTAATGAAGGTATAAAAATGGGATTTGAGAACATTGTTATACCAACTAG
AAACAAGGAAAAAATTGTAACTAAAAATAAAAATATTATTGGGGTATCATCATTAAGAGAAGCAGTAAGTAAAGTGTTTT
AA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(343-428)

AAA domain (AAA_25)

(60-219)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

52.967

100

0.532
  radA Streptococcus pneumoniae Rx1

50.441

100

0.506
  radA Streptococcus pneumoniae D39

50.441

100

0.506
  radA Streptococcus pneumoniae R6

50.441

100

0.506
  radA Streptococcus pneumoniae TIGR4

50.441

100

0.506
  radA Streptococcus mitis NCTC 12261

50.441

100

0.506
  radA Streptococcus mitis SK321

50.22

100

0.503