Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   IGI31_RS01860 Genome accession   NZ_CP075465
Coordinates   406438..407115 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain NT1N34     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 401438..412115
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IGI31_RS01840 - 402387..403084 (+) 698 WP_223411729.1 IS1 family transposase -
  IGI31_RS01845 chiA 403102..405498 (+) 2397 Protein_368 bifunctional chitinase/lysozyme -
  IGI31_RS01850 bfd 405667..405861 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  IGI31_RS01855 bfr 405933..406409 (+) 477 WP_000675504.1 bacterioferritin -
  IGI31_RS01860 pilD 406438..407115 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  IGI31_RS01865 gspM 407115..407576 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  IGI31_RS01870 gspL 407573..408736 (-) 1164 WP_193107731.1 type II secretion system protein GspL -
  IGI31_RS01875 gspK 408751..409734 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  IGI31_RS23085 - 409727..409816 (-) 90 Protein_375 type II secretion system protein J -
  IGI31_RS01885 gspJ 410573..411091 (-) 519 Protein_377 type II secretion system minor pseudopilin GspJ -
  IGI31_RS01890 gspI 411084..411461 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  IGI31_RS01895 gspH 411458..411967 (-) 510 WP_053295483.1 type II secretion system minor pseudopilin GspH -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=498034 IGI31_RS01860 WP_000178161.1 406438..407115(-) (pilD) [Escherichia coli strain NT1N34]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=498034 IGI31_RS01860 WP_000178161.1 406438..407115(-) (pilD) [Escherichia coli strain NT1N34]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTAGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378