Detailed information NTG0049798

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: HK86_RS03170
• Genome accession: NC_021847
• Coordinates: 652758..654962 (-)
• Length: 734 a.a.
• NCBI ID: WP_020840006.1
• Uniprot ID: -
• Organism: Vibrio parahaemolyticus O1:Kuk str. FDA_R31
• Function: DNA puliing through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 647758..659962

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HK86_RS03150 (M634_03240)	hslU	648625..649956 (-)	1332	WP_020840004.1	HslU--HslV peptidase ATPase subunit	-
HK86_RS03155 (M634_03245)	hslV	649980..650531 (-)	552	WP_005489705.1	ATP-dependent protease subunit HslV	-
HK86_RS03160 (M634_03250)	ftsN	650696..651241 (-)	546	WP_005481405.1	cell division protein FtsN	-
HK86_RS03165 (M634_03260)	cytR	651418..652464 (-)	1047	WP_254894148.1	DNA-binding transcriptional regulator CytR	Regulator
HK86_RS03170 (M634_03265)	priA	652758..654962 (-)	2205	WP_020840006.1	primosomal protein N'	Machinery gene
HK86_RS03175 (M634_03270)	rpmE	655257..655478 (+)	222	WP_005457203.1	50S ribosomal protein L31	-
HK86_RS03180 (M634_03275)	rpsJ	655936..656247 (+)	312	WP_004410492.1	30S ribosomal protein S10	-
HK86_RS03185 (M634_03280)	rplC	656262..656891 (+)	630	WP_005456132.1	50S ribosomal protein L3	-
HK86_RS03190 (M634_03285)	rplD	656909..657511 (+)	603	WP_005379556.1	50S ribosomal protein L4	-
HK86_RS03195 (M634_03290)	rplW	657508..657810 (+)	303	WP_004398471.1	50S ribosomal protein L23	-
HK86_RS03200 (M634_03295)	rplB	657826..658650 (+)	825	WP_005489461.1	50S ribosomal protein L2	-
HK86_RS03205 (M634_03300)	rpsS	658672..658950 (+)	279	WP_004394525.1	30S ribosomal protein S19	-
HK86_RS03210 (M634_03305)	rplV	658961..659293 (+)	333	WP_005383164.1	50S ribosomal protein L22	-

Sequence

Protein

• Length: 734 a.a.
• Molecular weight: 82229.73 Da
• Isoelectric Point: 8.5714

>NTDB_id=49798 HK86_RS03170 WP_020840006.1 652758..654962(-) (priA) [Vibrio parahaemolyticus O1:Kuk str. FDA_R31]
MRPSIARVALPVPLDKQFDYAIPGHLFPIIGGRVSVPFGRQTLVGIVTAMVNHSDFPKDQLKPIKAVLDSQPVWSEKLYS
LLTWCSQFYQYPLGDTLHNAMPAALRKGKPADFATLQEWQITESGKDKLMQGLDRRAVKQQKVLQMLVNGALPHQEFVDQ
EIASTVLKSLEEKGWIERIEKKPVITQWGQHVECDVEKPKLNHEQALAIASVNSQTGFACYLLEGVTGSGKTEVYLNLIK
PVLEKGKQALVLVPEIGLTPQTINRFKRRFNVPVDVIHSGLNETERLNAWLSARDKAAGIIIGTRSALLAPFADLGIIIV
DEEHDTSYKQQDSLRYHARDVAVMRAHKEQVPIVLGSATPALETLHNALSGKYHHLTLTQRAGSAVPTTNKVLDVKGQYL
ESGLSAPLIAEMRKHLKAGNQVMLFLNRRGFSPALMCHECGWIAECKRCDAYYTFHQYSNEIRCHHCGSQQPVIHQCQGC
GSTQLVTVGVGTEQLEQQLAQLFPEYKAIRIDRDSTRRKGSLEDALDSIRKGEYQILIGTQMLAKGHHFPNVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVVLQTHHPEHSLLQALLEKDYRHFAMTALEERKLAQLPPYSFLTLF
KAEANQSEIVEDFLRQVRFTLESHPLFDDTCIVLGPTPSPLAKRAGKYRWQLLLQTQHRSLMQKLLSSAKPAIELLPNAK
KVRWNLDIEPQDLS

Nucleotide

• Length: 2205 bp

>NTDB_id=49798 HK86_RS03170 WP_020840006.1 652758..654962(-) (priA) [Vibrio parahaemolyticus O1:Kuk str. FDA_R31]
ATGCGTCCATCCATTGCTAGAGTGGCACTGCCCGTACCTCTCGACAAACAATTTGACTATGCCATCCCAGGACACCTGTT
TCCTATCATTGGCGGGCGCGTATCCGTACCTTTTGGACGTCAAACGTTAGTTGGCATCGTCACCGCGATGGTTAATCACT
CGGACTTTCCCAAAGATCAACTCAAGCCAATCAAAGCAGTTCTTGATTCTCAGCCGGTATGGTCTGAAAAACTTTATTCG
TTGCTGACTTGGTGCAGCCAGTTCTACCAATACCCACTTGGAGATACGCTACACAATGCGATGCCGGCAGCTCTGCGTAA
AGGAAAGCCTGCAGATTTTGCGACGCTACAAGAGTGGCAAATTACCGAATCCGGTAAAGACAAGCTGATGCAAGGGCTTG
ATCGTCGAGCGGTCAAACAACAGAAGGTCTTGCAAATGTTGGTTAACGGCGCTTTGCCTCACCAGGAATTTGTCGACCAA
GAGATAGCCTCAACAGTACTAAAGTCTTTAGAAGAGAAAGGCTGGATTGAACGTATCGAGAAGAAGCCTGTAATCACCCA
GTGGGGACAGCACGTTGAATGCGACGTCGAGAAGCCAAAGCTCAACCACGAACAAGCGCTGGCTATTGCGAGTGTAAATA
GCCAAACAGGTTTTGCTTGCTATTTATTAGAGGGTGTCACGGGCTCAGGTAAGACAGAAGTGTACCTCAACCTGATCAAA
CCGGTCTTAGAGAAAGGTAAACAAGCTTTGGTTTTAGTACCAGAGATTGGCTTAACACCACAAACCATCAACCGCTTTAA
ACGCCGTTTTAATGTGCCAGTTGACGTTATCCACTCCGGATTAAACGAGACGGAGCGTCTGAATGCGTGGCTTTCTGCAC
GCGACAAAGCGGCCGGAATCATTATCGGAACACGCTCTGCCCTACTCGCGCCTTTTGCTGATCTCGGGATTATCATTGTG
GACGAGGAACATGACACCTCTTACAAACAGCAAGATAGCTTGCGTTATCATGCACGCGATGTTGCTGTAATGCGCGCTCA
TAAAGAACAAGTTCCGATTGTTCTGGGCTCAGCCACACCTGCACTGGAGACATTGCACAATGCCTTGTCAGGCAAGTATC
ACCATTTAACCCTGACCCAACGCGCAGGCTCTGCGGTTCCTACTACTAATAAAGTGCTCGACGTCAAAGGTCAGTATTTA
GAAAGTGGTTTATCTGCCCCATTGATTGCGGAAATGCGCAAACACCTTAAAGCAGGCAATCAGGTGATGTTGTTTCTTAA
CCGTCGTGGTTTCTCCCCTGCTCTCATGTGCCATGAATGTGGTTGGATTGCCGAATGCAAACGTTGTGATGCTTATTACA
CCTTCCACCAATACAGCAATGAGATTCGCTGCCACCATTGTGGTTCACAGCAACCAGTGATTCATCAGTGTCAGGGCTGT
GGTTCAACTCAACTGGTTACCGTTGGTGTCGGTACCGAACAGTTAGAACAACAATTGGCACAACTGTTCCCCGAATACAA
AGCCATCCGTATCGACAGGGATAGTACGCGCCGCAAAGGTAGCTTAGAAGACGCGTTGGACTCCATCCGCAAGGGTGAAT
ATCAAATTTTAATTGGCACTCAAATGTTAGCTAAGGGACACCATTTCCCCAATGTAACGCTCGTTGCCTTGCTAGATGTT
GATGGCTCTCTATATAGTAGCGACTTCCGCGCCTCCGAAAGATTAGCGCAGCTATTCATTCAAGTCGCAGGGCGAGCCGG
ACGTGCAAGTAAGCCGGGGGAAGTAGTTTTGCAAACTCACCATCCTGAGCACAGTTTGTTGCAAGCCTTATTGGAAAAAG
ACTACCGACACTTCGCTATGACGGCATTAGAGGAACGTAAACTGGCGCAGTTGCCACCGTACAGTTTCCTGACTCTATTT
AAAGCCGAAGCGAATCAGAGCGAGATCGTAGAAGATTTTCTGCGCCAAGTTCGCTTTACCTTAGAATCTCACCCTTTGTT
TGACGACACCTGCATAGTGCTTGGTCCAACACCATCGCCTCTCGCCAAACGCGCAGGCAAATACCGTTGGCAGCTGTTAC
TCCAAACCCAGCACCGTTCATTAATGCAAAAGTTATTAAGCAGTGCCAAACCTGCTATTGAGTTGTTGCCTAATGCCAAA
AAAGTTCGCTGGAATTTAGACATAGAACCACAAGATCTCAGCTAG

Domains

Predicted by InterProScan.

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (446-472)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (7-102)

• Helicase conserved C-terminal domain (Helicase_C) (500-589)

• Primosomal protein N C-terminal domain (PriA_C) (633-730)

• DEAD/DEAH box helicase (DEAD) (220-365)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Acinetobacter baumannii D1279779	43.514	100	0.439
priA	Bacillus subtilis subsp. subtilis str. 168	34.131	100	0.369