Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   IGI19_RS02500 Genome accession   NZ_CP075462
Coordinates   435829..436506 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain NT1F10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 430829..441506
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IGI19_RS02485 chiA 432196..434889 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  IGI19_RS02490 bfd 435058..435252 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  IGI19_RS02495 bfr 435324..435800 (+) 477 WP_000675504.1 bacterioferritin -
  IGI19_RS02500 pilD 435829..436506 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  IGI19_RS02505 gspM 436506..436967 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  IGI19_RS02510 gspL 436964..438127 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  IGI19_RS02515 gspK 438142..439125 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  IGI19_RS02520 gspJ 439118..439705 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  IGI19_RS02525 gspI 439698..440075 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  IGI19_RS02530 gspH 440072..440581 (-) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  IGI19_RS02535 gspG 440589..441026 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=497956 IGI19_RS02500 WP_000178161.1 435829..436506(-) (pilD) [Escherichia coli strain NT1F10]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=497956 IGI19_RS02500 WP_000178161.1 435829..436506(-) (pilD) [Escherichia coli strain NT1F10]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378