Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   KIL00_RS13905 Genome accession   NZ_CP075344
Coordinates   2652004..2652414 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis subsp. subtilis strain A1 - Midalam     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2647004..2657414
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIL00_RS13875 (KIL00_13875) - 2647092..2647232 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  KIL00_RS13880 (KIL00_13880) yqeF 2647538..2648269 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  KIL00_RS13885 (KIL00_13885) cwlH 2648521..2649273 (-) 753 WP_069837642.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  KIL00_RS13890 (KIL00_13890) yqeD 2649460..2650086 (+) 627 WP_014480319.1 TVP38/TMEM64 family protein -
  KIL00_RS13895 (KIL00_13895) gnd 2650105..2650998 (-) 894 WP_069837643.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  KIL00_RS13900 (KIL00_13900) yqeB 2651249..2651971 (+) 723 WP_014480321.1 hypothetical protein -
  KIL00_RS13905 (KIL00_13905) nucA/comI 2652004..2652414 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  KIL00_RS13910 (KIL00_13910) - 2652610..2652957 (+) 348 Protein_2702 sigma-70 family RNA polymerase sigma factor -
  KIL00_RS13915 (KIL00_13915) spoIVCA 2652988..2654448 (-) 1461 WP_250629559.1 site-specific DNA recombinase SpoIVCA -
  KIL00_RS22150 - 2654406..2654584 (-) 179 Protein_2704 hypothetical protein -
  KIL00_RS13920 (KIL00_13920) arsC 2654939..2655358 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  KIL00_RS13925 (KIL00_13925) acr3 2655370..2656410 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  KIL00_RS13930 (KIL00_13930) yqcK 2656433..2656873 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  KIL00_RS13935 (KIL00_13935) arsR 2656934..2657251 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=497815 KIL00_RS13905 WP_009967785.1 2652004..2652414(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain A1 - Midalam]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=497815 KIL00_RS13905 WP_009967785.1 2652004..2652414(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain A1 - Midalam]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529