Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   IH404_RS02935 Genome accession   NZ_CP063780
Coordinates   660173..661498 (+) Length   441 a.a.
NCBI ID   WP_194440640.1    Uniprot ID   A0A7S7S7D4
Organism   Pseudomonas sp. OST1909     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 655173..666498
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IH404_RS02915 (IH404_02915) - 655232..657697 (-) 2466 WP_005784035.1 penicillin-binding protein 1A -
  IH404_RS02920 (IH404_02920) pilM 657928..658584 (+) 657 WP_194439901.1 type IV pilus assembly protein PilM -
  IH404_RS02925 (IH404_02925) - 658581..659135 (+) 555 WP_194439904.1 PilN domain-containing protein -
  IH404_RS02930 (IH404_02930) - 659132..660103 (+) 972 WP_005784039.1 pilus assembly protein PilP -
  IH404_RS02935 (IH404_02935) pilQ 660173..661498 (+) 1326 WP_194440640.1 type IV pilus secretin PilQ family protein Machinery gene
  IH404_RS02940 (IH404_02940) aroK 661503..662021 (+) 519 WP_005784043.1 shikimate kinase AroK -
  IH404_RS02945 (IH404_02945) aroB 662147..663247 (+) 1101 WP_005784045.1 3-dehydroquinate synthase -
  IH404_RS02950 (IH404_02950) - 663258..664829 (+) 1572 WP_005784047.1 AAA family ATPase -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 47866.72 Da        Isoelectric Point: 4.9435

>NTDB_id=496768 IH404_RS02935 WP_194440640.1 660173..661498(+) (pilQ) [Pseudomonas sp. OST1909]
MAAAVPNRLDLIHLPPPDSAASVSYTGTRLNLNFQDIDLRAVLQQIADVAGLNLVTSDEVQGSITLRLKDVPWDQALDLV
LQTKGLDKRVKAGVLLVAPADELAARELLALESRKQMAELAPLRRELLQVNYAKAADLAKLFQSVTGFEGVTDERGSVAV
DDRTNNIIAYQTGERLEELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGRLSHGRGSVAGVSKPVADGAEPPQ
NPPGSPFVDLGSLTGTSGLGVAYITDNLLLDLELTAMEKTGNGEIVSQPKVVTSDKETARILKGTEIPYQESTSQGATSV
SFKEASLSLEVTPQITPDDHVIMAVKVTKDEPDYLNRLNDVPPIKKNEVNAKVLVRDGETIVIGGVFSNTQSKVVDKVPF
LGDVPYLGRLFRRDVLAEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=496768 IH404_RS02935 WP_194440640.1 660173..661498(+) (pilQ) [Pseudomonas sp. OST1909]
ATGGCTGCTGCTGTGCCCAATCGCCTGGACCTGATCCACCTGCCGCCGCCCGACAGTGCAGCGTCGGTCAGCTATACAGG
CACCCGGCTGAATCTGAATTTCCAGGACATCGACCTGCGGGCGGTCTTGCAGCAAATCGCCGATGTCGCCGGGCTCAACC
TGGTGACCAGTGATGAAGTGCAGGGCTCAATTACCCTGCGGCTCAAGGATGTGCCTTGGGATCAGGCGCTGGATCTGGTG
CTGCAAACCAAAGGTCTGGATAAGCGCGTGAAAGCCGGTGTGTTGCTGGTGGCACCTGCGGATGAGTTGGCCGCTCGCGA
ACTGCTGGCGCTGGAGTCGCGCAAGCAGATGGCGGAACTCGCACCGCTGCGTCGGGAGCTGCTGCAGGTCAACTATGCCA
AGGCGGCGGACCTGGCCAAGCTGTTTCAGTCGGTCACAGGTTTTGAAGGCGTCACGGATGAGCGCGGCTCGGTGGCGGTG
GATGATCGCACCAACAACATCATCGCCTACCAGACCGGCGAACGGCTCGAAGAGCTGCGGCGTATCGTGGCGCAACTGGA
TATTCCCGTACGCCAAGTGATGATCGAGGCGCGAATTGTCGAGGCCAATGTCGACTACGACAAAAGCCTGGGCGTGCGCT
GGGGTGGGCGGCTCAGTCATGGCAGGGGAAGTGTCGCAGGTGTTTCCAAGCCCGTGGCAGACGGCGCCGAACCGCCGCAA
AACCCGCCCGGCTCCCCGTTTGTCGATTTGGGTTCGCTGACCGGTACCTCGGGTCTGGGCGTGGCCTACATCACCGACAA
TCTACTGCTGGACCTTGAGCTGACAGCCATGGAGAAAACCGGCAACGGCGAAATCGTCTCGCAGCCCAAGGTGGTCACGT
CCGACAAGGAGACGGCACGCATCCTCAAGGGCACCGAAATTCCCTATCAAGAGTCCACCTCCCAGGGCGCTACCTCGGTA
TCGTTCAAGGAGGCTTCGCTTTCGCTGGAGGTCACGCCGCAAATCACCCCCGACGACCACGTGATCATGGCGGTCAAGGT
CACCAAGGATGAGCCCGACTACTTGAACAGGCTCAATGACGTACCGCCGATCAAGAAAAACGAGGTCAACGCCAAGGTGC
TGGTCAGGGATGGCGAGACCATCGTCATCGGTGGGGTTTTCTCCAATACCCAAAGCAAAGTGGTAGATAAAGTGCCATTT
TTGGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGCGATGTGCTGGCGGAGAAAAAATCCGAGCTGCTGGTATTCCT
GACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(276-432)

Bacterial type II/III secretion system short domain (Secretin_N)

(127-192)

Secretin and TonB N terminus short domain (STN)

(54-99)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7S7S7D4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.068

100

0.701
  comQ Acinetobacter baylyi ADP1

41.545

100

0.451
  pilQ Acinetobacter baumannii strain A118

39.351

100

0.44
  pilQ Acinetobacter baumannii D1279779

39.351

100

0.44
  pilQ Legionella pneumophila strain Lp02

45.346

95.011

0.431
  pilQ Legionella pneumophila strain ERS1305867

44.869

95.011

0.426
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

43.488

97.506

0.424
  pilQ Vibrio cholerae strain A1552

43.488

97.506

0.424
  pilQ Vibrio campbellii strain DS40M4

43.972

95.918

0.422