Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   KIL91_RS25995 Genome accession   NZ_CP074982
Coordinates   5032027..5033460 (+) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain 12689     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5027027..5038460
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIL91_RS25965 (KIL91_25625) yrbG 5027585..5028562 (+) 978 WP_000922901.1 calcium/sodium antiporter -
  KIL91_RS25970 (KIL91_25630) kdsD 5028576..5029562 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  KIL91_RS25975 (KIL91_25635) kdsC 5029583..5030149 (+) 567 WP_000030018.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  KIL91_RS25980 (KIL91_25640) lptC 5030146..5030721 (+) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  KIL91_RS25985 (KIL91_25645) lptA 5030690..5031247 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  KIL91_RS25990 (KIL91_25650) lptB 5031254..5031979 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  KIL91_RS25995 (KIL91_25655) rpoN/rpoN1 5032027..5033460 (+) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  KIL91_RS26000 (KIL91_25660) hpf 5033483..5033770 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  KIL91_RS26005 (KIL91_25665) ptsN 5033888..5034379 (+) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  KIL91_RS26010 (KIL91_25670) rapZ 5034425..5035279 (+) 855 WP_000243741.1 RNase adapter RapZ -
  KIL91_RS26015 (KIL91_25675) npr 5035276..5035548 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  KIL91_RS26020 (KIL91_25680) yrbL 5035762..5036394 (+) 633 WP_032341768.1 PhoP regulatory network protein YrbL -
  KIL91_RS26025 (KIL91_25685) mtgA 5036391..5037119 (-) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  KIL91_RS26030 (KIL91_25690) elbB 5037116..5037769 (-) 654 WP_001299134.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=496684 KIL91_RS25995 WP_000809051.1 5032027..5033460(+) (rpoN/rpoN1) [Escherichia coli strain 12689]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=496684 KIL91_RS25995 WP_000809051.1 5032027..5033460(+) (rpoN/rpoN1) [Escherichia coli strain 12689]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGATGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
TGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAGGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTAGCGGCAAAAGATCTGCGTGACTGCCTGCTAATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGATTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACAGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCTCGCAATGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGCGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465