Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   IOD65_RS17140 Genome accession   NZ_CP063769
Coordinates   3589351..3590151 (-) Length   266 a.a.
NCBI ID   WP_002114699.1    Uniprot ID   -
Organism   Acinetobacter sp. Ac-14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3584351..3595151
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IOD65_RS17120 (IOD65_17120) - 3585416..3586114 (-) 699 WP_005066180.1 YfgM family protein -
  IOD65_RS17125 (IOD65_17125) hisS 3586143..3587435 (-) 1293 WP_005066179.1 histidine--tRNA ligase -
  IOD65_RS17130 (IOD65_17130) ispG 3587432..3588547 (-) 1116 WP_002115078.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  IOD65_RS17135 (IOD65_17135) - 3588569..3589360 (-) 792 WP_002114935.1 helix-turn-helix domain-containing protein -
  IOD65_RS17140 (IOD65_17140) pilF 3589351..3590151 (-) 801 WP_002114699.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  IOD65_RS17145 (IOD65_17145) rlmN 3590166..3591401 (-) 1236 WP_002115097.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  IOD65_RS17150 (IOD65_17150) ndk 3591525..3591956 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  IOD65_RS17155 (IOD65_17155) iscX 3592070..3592270 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  IOD65_RS17160 (IOD65_17160) - 3592370..3592921 (-) 552 WP_005066176.1 phosphatase PAP2 family protein -
  IOD65_RS17165 (IOD65_17165) - 3592946..3594235 (-) 1290 WP_080591623.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30114.38 Da        Isoelectric Point: 9.0417

>NTDB_id=496674 IOD65_RS17140 WP_002114699.1 3589351..3590151(-) (pilF) [Acinetobacter sp. Ac-14]
MSIPKLKTVLCMGIAVAFLVSGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDV
ANAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGVRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=496674 IOD65_RS17140 WP_002114699.1 3589351..3590151(-) (pilF) [Acinetobacter sp. Ac-14]
TTGAGTATTCCAAAGTTAAAAACGGTTTTATGTATGGGGATTGCAGTTGCATTTCTGGTAAGTGGTTGCCAGACAACGCA
TACGCAAAAAAAAGACCCTGAGAAAGCGGTAAAGGTGCGTACACAACTTGCTGCTGAACATATCCGTTCGGGTGACTTGG
ACTCAGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGCGATGCTACTGCAAATATGATGATGGGTATTTTG
CTACAGCAAGAAGGTAGCAAACCCAATTTAGAAAAAGCAGAACATTATTTTAAAAGAGCAATTTCATCTGAACCGGATAA
TGCTCAAGCACATAATAATTATGGTACTTATTTATATCAAATGGAGCGTTATAATGATGCGGTTGAGCAATTTCGTATTG
CTGGCGCAACATTAGGTTATGACCAACGCTATCAGGCATTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GCGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTGTTAGTAAACCAG
TTACGTGCTTTGTTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

93.985

100

0.94
  pilF Acinetobacter baylyi ADP1

76.154

97.744

0.744