Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   IM712_RS03870 Genome accession   NZ_CP063768
Coordinates   794011..795390 (-) Length   459 a.a.
NCBI ID   WP_014416742.1    Uniprot ID   I2C0L1
Organism   Bacillus velezensis strain KMU01     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 789011..800390
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IM712_RS03850 ispF 790531..791007 (-) 477 WP_003156407.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  IM712_RS03855 ispD 791000..791698 (-) 699 WP_077721695.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  IM712_RS03860 - 791711..792811 (-) 1101 WP_003156403.1 PIN/TRAM domain-containing protein -
  IM712_RS03865 disA 792925..794007 (-) 1083 WP_003156401.1 DNA integrity scanning diadenylate cyclase DisA -
  IM712_RS03870 radA 794011..795390 (-) 1380 WP_014416742.1 DNA repair protein RadA Machinery gene
  IM712_RS03875 clpC 795484..797915 (-) 2432 Protein_732 ATP-dependent protease ATP-binding subunit ClpC -
  IM712_RS03880 - 797912..799003 (-) 1092 WP_003156398.1 protein arginine kinase -
  IM712_RS03885 - 799003..799560 (-) 558 WP_003156397.1 UvrB/UvrC motif-containing protein -
  IM712_RS03890 ctsR 799574..800038 (-) 465 WP_003156396.1 transcriptional regulator CtsR -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49547.98 Da        Isoelectric Point: 7.1760

>NTDB_id=496577 IM712_RS03870 WP_014416742.1 794011..795390(-) (radA) [Bacillus velezensis strain KMU01]
MAKSKTKFICHSCGYESAKWMGKCPGCGAWNTMVEETIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVHTKLEEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLADTAGSVLYISGEESVKQTKLRADRLGINSQMLHVLSETDMEYIS
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGASGSSIVASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPEANADGWTIPKGIEVVGVANVAEALRTSLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=496577 IM712_RS03870 WP_014416742.1 794011..795390(-) (radA) [Bacillus velezensis strain KMU01]
ATGGCTAAATCAAAAACAAAATTCATCTGCCACTCATGCGGTTATGAATCCGCAAAATGGATGGGAAAGTGCCCGGGCTG
CGGGGCTTGGAATACAATGGTGGAAGAAACCATAAAAAAAGCGCCGGCGAATCGGCGGGCGGCTTTCTCCCACTCCGTTC
AAACCGTCCAGAAACCATCACCCATTACATCAATTGAAACTTCAGAAGAACCCCGCGTTCACACAAAGCTCGAAGAATTC
AACAGAGTGCTTGGCGGCGGCGTTGTGAAAGGCTCGTTAGTATTGATCGGCGGCGATCCCGGGATCGGAAAATCAACGCT
CCTTCTCCAAGTATCCGCACAATTAGCAGATACAGCCGGCAGCGTGCTTTACATTTCAGGGGAAGAATCCGTGAAGCAAA
CGAAGCTGCGCGCCGACAGACTTGGAATCAACAGCCAAATGTTACATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAAACCCGCATTTGTTGTCGTAGACTCCATTCAGACCGTTTACCAAAGTGATATCACCTCGGC
TCCCGGCAGTGTATCACAAGTAAGAGAGTGTACGGCCGAATTGATGAAAATCGCGAAAACAAACGGCATACCTATTTTTA
TCGTCGGACACGTCACAAAAGAAGGATCGATCGCAGGGCCGAGATTGCTTGAGCACATGGTCGATACCGTTCTATACTTT
GAAGGAGAGCGGCATCATACATTCCGTATTTTGCGGGCCGTAAAAAACCGTTTCGGCTCAACAAATGAAATGGGCATCTT
TGAAATGCGGGAAGAGGGGCTTACGGAAGTGCTCAACCCTTCTGAAATTTTCTTGGAAGAGCGTTCTGCCGGGGCATCGG
GTTCGAGTATCGTTGCGTCAATGGAAGGAACGAGACCGATTCTGGTTGAAATCCAGGCGCTCATATCACCGACCAGTTTT
GGAAACCCGCGCCGCATGGCTACAGGCATTGACCACAACAGGGTATCGTTAATTATGGCTGTGCTTGAGAAGCGGGTGGG
TCTTTTGCTTCAAAATCAAGATGCATATTTAAAAGTCGCCGGCGGGGTCAAACTGGATGAACCGGCGATTGATTTGGCAG
TAGCCGTCAGCATTGCCTCAAGCTTCAGAGATACACCGCCGAATCCCGCAGATTGCTTTATCGGTGAAGTGGGACTGACA
GGAGAAGTCCGCAGGGTGTCAAGAATCGAACAGCGCGTAAAAGAAGCGGCAAAACTCGGCTTCAAGCGCATGATCATACC
TGAGGCGAATGCAGACGGGTGGACAATACCAAAAGGGATTGAGGTTGTCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATCATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)

AAA domain (AAA_25)

(73-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C0L1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

93.45

99.782

0.932
  radA Streptococcus pneumoniae Rx1

63.797

98.693

0.63
  radA Streptococcus pneumoniae D39

63.797

98.693

0.63
  radA Streptococcus pneumoniae R6

63.797

98.693

0.63
  radA Streptococcus pneumoniae TIGR4

63.797

98.693

0.63
  radA Streptococcus mitis NCTC 12261

63.797

98.693

0.63
  radA Streptococcus mitis SK321

63.576

98.693

0.627