Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   KIL96_RS00645 Genome accession   NZ_CP074961
Coordinates   133527..134195 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain 01_3327     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 128527..139195
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIL96_RS00615 (KIL96_00615) yicC 128546..129409 (-) 864 WP_000621336.1 YicC/YloC family endoribonuclease -
  KIL96_RS00620 (KIL96_00620) rph 129536..130252 (+) 717 WP_001247089.1 ribonuclease PH -
  KIL96_RS00625 (KIL96_00625) pyrE 130318..130959 (+) 642 WP_000806161.1 orotate phosphoribosyltransferase -
  KIL96_RS00630 (KIL96_00630) slmA 130996..131592 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  KIL96_RS00635 (KIL96_00635) dut 131699..132157 (-) 459 WP_000976070.1 dUTP diphosphatase -
  KIL96_RS00640 (KIL96_00640) coaBC 132135..133355 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  KIL96_RS00645 (KIL96_00645) radC 133527..134195 (+) 669 WP_001297375.1 RadC family protein Machinery gene
  KIL96_RS00650 (KIL96_00650) rpmB 134412..134648 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  KIL96_RS00655 (KIL96_00655) rpmG 134669..134836 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  KIL96_RS00660 (KIL96_00660) mutM 134934..135743 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  KIL96_RS00665 (KIL96_00665) coaD 135782..136261 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  KIL96_RS00670 (KIL96_00670) waaA 136269..137546 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  KIL96_RS00675 (KIL96_00675) rfaQ 137959..139017 (+) 1059 WP_000360406.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=496221 KIL96_RS00645 WP_001297375.1 133527..134195(+) (radC) [Escherichia coli strain 01_3327]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=496221 KIL96_RS00645 WP_001297375.1 133527..134195(+) (radC) [Escherichia coli strain 01_3327]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGTTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGCGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496