Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   IQE94_RS01545 Genome accession   NZ_CP063659
Coordinates   336496..336957 (-) Length   153 a.a.
NCBI ID   WP_223342564.1    Uniprot ID   -
Organism   Synechocystis sp. PCC 7339     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 324538..336168 336496..336957 flank 328


Gene organization within MGE regions


Location: 324538..336957
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IQE94_RS01505 - 324586..324885 (-) 300 Protein_296 transposase -
  IQE94_RS01510 (IQE94_01485) - 325131..326231 (-) 1101 WP_223342557.1 IS4 family transposase -
  IQE94_RS01515 (IQE94_01490) - 326476..327372 (+) 897 WP_223342558.1 class I SAM-dependent methyltransferase -
  IQE94_RS01520 (IQE94_01495) - 327383..329851 (+) 2469 WP_223342559.1 tetratricopeptide repeat protein -
  IQE94_RS01525 (IQE94_01500) - 329854..332337 (+) 2484 WP_223342560.1 CDC27 family protein -
  IQE94_RS01530 (IQE94_01505) - 332340..334787 (+) 2448 WP_223342561.1 tetratricopeptide repeat protein -
  IQE94_RS01535 (IQE94_01510) - 334809..335624 (-) 816 WP_223342562.1 hypothetical protein -
  IQE94_RS01540 (IQE94_01515) - 335659..336237 (-) 579 WP_223342563.1 type IV pilin protein -
  IQE94_RS01545 (IQE94_01520) pilA/pilA1 336496..336957 (-) 462 WP_223342564.1 type IV pilin protein Machinery gene

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 16100.26 Da        Isoelectric Point: 10.2552

>NTDB_id=496179 IQE94_RS01545 WP_223342564.1 336496..336957(-) (pilA/pilA1) [Synechocystis sp. PCC 7339]
MTSNFKFKLLSHLTQKKGEKGFTLIELLVVVIIIGVLAAIALPNLLNQVGKARMSEGKSTLGAINRGQQVYRTENPTFAS
NLTSLDTKVPTTGKFFSYSAASATSTGVVNTASALSAEGDNTTSITTTITYTPTDGTFTLTNSAQTANKSGGW

Nucleotide


Download         Length: 462 bp        

>NTDB_id=496179 IQE94_RS01545 WP_223342564.1 336496..336957(-) (pilA/pilA1) [Synechocystis sp. PCC 7339]
ATGACCAGCAATTTTAAATTTAAACTCCTCTCCCACCTCACTCAGAAAAAAGGAGAAAAAGGTTTTACCCTGATTGAACT
ATTAGTAGTAGTTATTATTATTGGTGTATTGGCTGCTATTGCCTTGCCCAATCTCTTGAACCAAGTAGGTAAAGCCCGGA
TGTCTGAGGGGAAAAGTACCTTAGGTGCCATTAATCGTGGCCAACAGGTGTATCGAACTGAGAATCCAACATTTGCAAGC
AACTTAACCTCTCTAGATACAAAAGTGCCGACAACCGGAAAGTTTTTTAGCTATTCGGCTGCAAGTGCCACCAGTACCGG
CGTTGTAAACACTGCATCAGCTCTTTCTGCTGAGGGTGATAACACAACATCCATTACTACTACCATCACTTATACACCAA
CTGATGGCACTTTCACCTTAACTAACTCTGCTCAAACTGCTAATAAGTCTGGTGGCTGGTAG

Domains

Predicted by InterproScan.

Type IV pilin-like G and H, putative (Pilin_GH)

(55-131)

Prokaryotic N-terminal methylation motif (N_methyl)

(17-42)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Synechocystis sp. PCC 6803

59.542

85.621

0.51