Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   INS04_RS02140 Genome accession   NZ_CP063441
Coordinates   466451..467884 (-) Length   477 a.a.
NCBI ID   WP_193805567.1    Uniprot ID   -
Organism   Enterobacter asburiae strain SD4L     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 461451..472884
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INS04_RS02110 (INS04_02110) elbB 462983..463636 (+) 654 WP_193807208.1 isoprenoid biosynthesis glyoxalase ElbB -
  INS04_RS02115 (INS04_02115) mtgA 463633..464358 (+) 726 WP_029739613.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  INS04_RS02120 (INS04_02120) npr 464398..464670 (-) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  INS04_RS02125 (INS04_02125) rapZ 464667..465521 (-) 855 WP_008501442.1 RNase adapter RapZ -
  INS04_RS02130 (INS04_02130) ptsN 465567..466058 (-) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  INS04_RS02135 (INS04_02135) hpf 466141..466428 (-) 288 WP_008501444.1 ribosome hibernation promoting factor -
  INS04_RS02140 (INS04_02140) rpoN/rpoN1 466451..467884 (-) 1434 WP_193805567.1 RNA polymerase factor sigma-54 Machinery gene
  INS04_RS02145 (INS04_02145) lptB 467932..468657 (-) 726 WP_033146751.1 LPS export ABC transporter ATP-binding protein -
  INS04_RS02150 (INS04_02150) lptA 468664..469218 (-) 555 WP_033146750.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  INS04_RS02155 (INS04_02155) lptC 469187..469762 (-) 576 WP_023309357.1 LPS export ABC transporter periplasmic protein LptC -
  INS04_RS02160 (INS04_02160) kdsC 469759..470325 (-) 567 WP_023309356.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  INS04_RS02165 (INS04_02165) kdsD 470340..471326 (-) 987 WP_023333606.1 arabinose-5-phosphate isomerase KdsD -
  INS04_RS02170 (INS04_02170) - 471340..472317 (-) 978 WP_193805568.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54167.92 Da        Isoelectric Point: 4.3173

>NTDB_id=495645 INS04_RS02140 WP_193805567.1 466451..467884(-) (rpoN/rpoN1) [Enterobacter asburiae strain SD4L]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDEEIELEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWLDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=495645 INS04_RS02140 WP_193805567.1 466451..467884(-) (rpoN/rpoN1) [Enterobacter asburiae strain SD4L]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCGATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
ACTGTCCACGTTAGAACTTCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAACCCCCTGCTGGAACAAACCGATCTTCATG
ACGAGGTAGACGCTCAGCAGTCACAGGATACAGAAGCGCTCGACACCGCGGATGCTCTCGAACAAAAAGAGATGCCCGAC
GAGCTTCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACCCCTTCCGGTACGCGTGCGGACTACCAGGACGA
TGAGCTACCGGTCTATCAGGGAGAAACCACTCAGTCGCTGCAGGATTACCTGATGTGGCAGGTAGAGCTTACCCCCTTCT
CCGATACCGACCGCGCGATTGCCACCTCAATTGTCGATGCCGTTGACGACACCGGCTATTTGACCGTCACGCTGGACGAT
ATCCTTGAAAGCATGGGCGACGAAGAGATTGAGCTTGAGGAGATCGAAGCCGTTCTGAAGCGCATCCAGCGTTTCGATCC
GGTAGGCGTGGCCGCAAAAGATCTGCGCGACTGTCTGCTGATCCAGCTTTCTCAGTTCAGCAAAGAGACGCCATGGCTCG
ACGAGGCGCGTTTAATCATCAGCGATCATCTGGATCTGTTGGCCAACCACGATTTCCGCAGCCTGATGCGCGTCACACGC
CTGAAGGAAGAGGTGCTGAAAGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGCCCCGGTCAGTCGATCCAGACCAG
CGAACCCGAGTACGTCATCCCGGACGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAATTCAGACAGCA
TCCCTCGTCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGCGCGTAACGACTCCGACAATCAATATATTCGC
AGCAATCTTCAGGAAGCGCGATGGCTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTCGAGCAGGGCGAAGAGTATATGAAACCGATGGTACTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGTGTCACCACGCAGAAGTATCTGCACAGTCCACGCGGTATATTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTTAATACCGAGGGCGGCGGCGAAGCGTCGTCAACGGCCATTCGTGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTAAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.437

100

0.48