Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   SPYALAB49_RS05535 Genome accession   NC_017596
Coordinates   1098826..1100631 (-) Length   601 a.a.
NCBI ID   WP_011054652.1    Uniprot ID   -
Organism   Streptococcus pyogenes Alab49     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 1078576..1125624 1098826..1100631 within 0


Gene organization within MGE regions


Location: 1078576..1125624
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPYALAB49_RS05450 (SPYALAB49_001116) - 1078614..1079888 (-) 1275 WP_014635557.1 polysaccharide deacetylase family protein -
  SPYALAB49_RS05455 (SPYALAB49_001117) - 1079983..1081410 (-) 1428 WP_011284931.1 NADP-dependent glyceraldehyde-3-phosphate dehydrogenase -
  SPYALAB49_RS05460 (SPYALAB49_001118) ptsP 1081595..1083328 (-) 1734 WP_014635558.1 phosphoenolpyruvate--protein phosphotransferase -
  SPYALAB49_RS05465 (SPYALAB49_001119) - 1083333..1083596 (-) 264 WP_002984031.1 phosphocarrier protein HPr -
  SPYALAB49_RS05470 (SPYALAB49_001120) - 1083989..1084207 (+) 219 WP_002989242.1 redoxin NrdH -
  SPYALAB49_RS05475 (SPYALAB49_001121) nrdE 1084227..1086386 (+) 2160 WP_002993430.1 class 1b ribonucleoside-diphosphate reductase subunit alpha -
  SPYALAB49_RS05480 (SPYALAB49_001123) nrdF 1086719..1087678 (+) 960 WP_011017933.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
  SPYALAB49_RS05485 (SPYALAB49_001124) - 1087740..1088966 (+) 1227 WP_014635559.1 chloride channel protein -
  SPYALAB49_RS05495 (SPYALAB49_001126) - 1089062..1090218 (+) 1157 WP_148256098.1 IS3 family transposase -
  SPYALAB49_RS05500 (SPYALAB49_001127) - 1090310..1091005 (-) 696 WP_002995750.1 CPBP family intramembrane glutamic endopeptidase -
  SPYALAB49_RS05505 (SPYALAB49_001128) - 1091024..1091785 (-) 762 WP_014635562.1 DUF3169 family protein -
  SPYALAB49_RS05510 (SPYALAB49_001129) - 1091782..1091997 (-) 216 WP_002989220.1 helix-turn-helix transcriptional regulator -
  SPYALAB49_RS05515 (SPYALAB49_001131) alaS 1092358..1094976 (-) 2619 WP_012560795.1 alanine--tRNA ligase -
  SPYALAB49_RS05520 (SPYALAB49_001132) prsA 1095363..1096418 (-) 1056 WP_002984003.1 peptidylprolyl isomerase PrsA -
  SPYALAB49_RS05525 (SPYALAB49_001133) - 1096481..1097188 (-) 708 WP_011284934.1 O-methyltransferase -
  SPYALAB49_RS05530 (SPYALAB49_001134) - 1097254..1098450 (-) 1197 WP_002995765.1 OFA family MFS transporter -
  SPYALAB49_RS05535 (SPYALAB49_001135) pepF 1098826..1100631 (-) 1806 WP_011054652.1 oligoendopeptidase F Regulator
  SPYALAB49_RS05540 (SPYALAB49_001136) - 1100644..1101606 (-) 963 WP_002983995.1 competence protein CoiA -
  SPYALAB49_RS05545 (SPYALAB49_001137) - 1101901..1102617 (-) 717 WP_002995774.1 pseudouridine synthase -
  SPYALAB49_RS05550 (SPYALAB49_001138) nagB 1102736..1103440 (-) 705 WP_009880651.1 glucosamine-6-phosphate deaminase -
  SPYALAB49_RS05555 (SPYALAB49_001139) queA 1103642..1104670 (+) 1029 WP_002995782.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  SPYALAB49_RS05560 (SPYALAB49_001140) - 1104677..1105897 (+) 1221 WP_002983986.1 DUF3114 domain-containing protein -
  SPYALAB49_RS10165 (SPYALAB49_001141) - 1106011..1106684 (-) 674 Protein_1060 serine hydrolase -
  SPYALAB49_RS10170 (SPYALAB49_001143) - 1106826..1107059 (-) 234 WP_002983979.1 hypothetical protein -
  SPYALAB49_RS05580 (SPYALAB49_001144) sodA 1107226..1107831 (-) 606 WP_002983977.1 superoxide dismutase SodA -
  SPYALAB49_RS05585 (SPYALAB49_001145) holA 1107928..1108968 (-) 1041 WP_011054659.1 DNA polymerase III subunit delta -
  SPYALAB49_RS05590 (SPYALAB49_001146) - 1109039..1111282 (-) 2244 WP_014635565.1 DNA internalization-related competence protein ComEC/Rec2 -
  SPYALAB49_RS05595 (SPYALAB49_001147) - 1111263..1111925 (-) 663 WP_002995626.1 ComEA family DNA-binding protein -
  SPYALAB49_RS05600 (SPYALAB49_001148) - 1112125..1112865 (-) 741 WP_014635566.1 1-acyl-sn-glycerol-3-phosphate acyltransferase -
  SPYALAB49_RS05605 (SPYALAB49_001149) - 1112983..1113759 (+) 777 WP_002994360.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  SPYALAB49_RS05610 (SPYALAB49_001150) - 1113749..1114027 (+) 279 WP_002989164.1 GIY-YIG nuclease family protein -
  SPYALAB49_RS05615 (SPYALAB49_001151) - 1114051..1116051 (-) 2001 WP_002994363.1 potassium transporter Kup -
  SPYALAB49_RS05620 (SPYALAB49_001152) - 1116178..1117797 (-) 1620 WP_014635567.1 DEAD/DEAH box helicase -
  SPYALAB49_RS05625 (SPYALAB49_001153) - 1118104..1119648 (-) 1545 WP_014635568.1 peptide chain release factor 3 -
  SPYALAB49_RS05630 (SPYALAB49_001154) - 1119896..1120591 (-) 696 WP_002994365.1 TIGR02206 family membrane protein -
  SPYALAB49_RS05635 (SPYALAB49_001155) murF 1120695..1122062 (-) 1368 WP_014635569.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
  SPYALAB49_RS05640 (SPYALAB49_001156) - 1122261..1123307 (-) 1047 WP_002994369.1 D-alanine--D-alanine ligase -
  SPYALAB49_RS05645 (SPYALAB49_001157) recR 1123408..1124004 (-) 597 WP_002983939.1 recombination mediator RecR -
  SPYALAB49_RS10175 - 1124051..1124233 (-) 183 WP_011888786.1 penicillin-binding protein -
  SPYALAB49_RS05655 (SPYALAB49_001158) - 1124793..1125572 (-) 780 WP_014635570.1 formate/nitrite transporter family protein -

Sequence

Protein


Download         Length: 601 a.a.        Molecular weight: 69341.19 Da        Isoelectric Point: 4.7093

>NTDB_id=49538 SPYALAB49_RS05535 WP_011054652.1 1098826..1100631(-) (pepF) [Streptococcus pyogenes Alab49]
MTDNRSHLEEKYTWDLSTIFATDKDWEAEVSDLATEIDAAKDFSGHLLDSSANLLTITDTYLELARRVEKVYVYAHMKND
QDTTVAKYQEYQAKASGLYAKFSEVFSFYDPEVMALSQEDYQAFLAETPDLKRYHHFFDKLFQTRDHVLSQAEEELLAGA
QEIFNGAEETFSILDNADIVFPVVKNDKGEDVELTHGNFISLMESKDRSIRQAAYEAMYSTYEQFQHTYAKTLQTNVKVQ
NYKARVHKYDSARQAAMAANFIPEAVYDTLLETVNKHLPLLHRYLKLRQEVLGLDDLKMYDVYTPLSETDLAIGYDEALE
KAEKVLAVFGKDYADRVHRAFTERWIDVHVNKGKRSGAYSGGSYDTNAFMLLNWQDTLDNLYTLVHETGHSLHSTFTRET
QPYVYGDYSIFLAEIASTTNENIMTEALLNEVQDEKERFAILNHYLDGFRGTVFRQTQFAEFEHAIHQADQKGEVLTSEY
LNQLYADLNEKYYGLSKKDNHFIQYEWARIPHFYYNYYVYQYATGFAAASYLADKIVHGTQDDIDHYLAYLKSGNSDYPL
EVIAKAGVDMAKGDYLEAAFKVFDERLTELEVLVSKGIHKS

Nucleotide


Download         Length: 1806 bp        

>NTDB_id=49538 SPYALAB49_RS05535 WP_011054652.1 1098826..1100631(-) (pepF) [Streptococcus pyogenes Alab49]
ATGACTGATAATCGCAGCCATTTAGAAGAAAAATACACTTGGGATTTGAGCACTATTTTTGCAACAGATAAGGATTGGGA
AGCTGAAGTCAGTGACTTAGCTACTGAAATTGACGCGGCTAAGGATTTTTCAGGTCATTTACTAGATTCTAGCGCTAATC
TGTTGACAATTACAGATACTTATTTAGAGTTAGCTCGTCGTGTTGAGAAAGTCTATGTTTATGCTCACATGAAAAATGAC
CAAGACACTACTGTTGCCAAGTATCAAGAATACCAAGCTAAGGCTTCAGGCTTGTATGCAAAGTTTAGCGAAGTGTTTTC
ATTCTATGACCCAGAAGTTATGGCATTGAGTCAGGAAGATTACCAAGCTTTTCTTGCGGAAACACCTGATTTAAAAAGGT
ATCATCATTTCTTTGACAAATTGTTCCAGACACGTGACCATGTGCTCAGCCAGGCTGAAGAGGAATTATTAGCAGGAGCC
CAAGAAATTTTCAACGGCGCTGAAGAAACCTTTAGTATTCTTGATAACGCTGATATTGTCTTTCCAGTAGTTAAAAATGA
TAAGGGGGAAGATGTTGAATTAACCCATGGTAACTTCATTAGCCTAATGGAATCTAAGGACCGTAGCATTCGCCAAGCTG
CTTATGAAGCCATGTATAGTACTTATGAACAGTTCCAACATACTTACGCTAAAACCTTGCAAACCAATGTGAAAGTGCAA
AACTATAAGGCGCGTGTTCATAAATACGATTCAGCACGTCAAGCTGCCATGGCTGCTAACTTTATTCCAGAAGCTGTTTA
CGATACCTTGTTAGAAACGGTTAATAAACATTTGCCACTCTTACACCGTTATTTGAAATTGCGTCAAGAGGTTCTTGGTT
TAGACGATTTGAAGATGTATGATGTTTACACACCATTATCTGAAACAGATTTAGCCATTGGTTATGATGAGGCTTTGGAA
AAGGCTGAGAAGGTTTTGGCTGTTTTTGGTAAGGATTACGCGGATCGTGTACATCGTGCTTTTACCGAACGTTGGATTGA
TGTTCACGTCAATAAAGGGAAGCGCTCTGGGGCTTATTCAGGTGGTTCCTATGATACCAATGCCTTCATGCTGTTGAACT
GGCAAGATACACTTGATAATCTTTACACACTTGTCCACGAAACAGGCCACAGTTTGCATTCTACTTTCACTCGTGAAACA
CAGCCTTATGTGTATGGGGATTATAGTATCTTCTTGGCAGAAATTGCATCCACAACGAATGAAAACATCATGACAGAAGC
TCTCTTAAATGAAGTGCAAGATGAGAAAGAACGCTTTGCCATTTTAAACCATTACCTCGATGGTTTCCGTGGAACAGTTT
TCCGTCAAACCCAATTTGCTGAGTTTGAACATGCCATTCATCAAGCTGATCAAAAAGGAGAAGTGTTAACAAGTGAATAC
CTTAACCAGCTATACGCTGATTTAAATGAAAAATATTATGGTTTAAGTAAAAAAGATAATCATTTTATTCAGTATGAATG
GGCACGTATTCCTCATTTCTATTACAATTATTATGTTTACCAATATGCAACAGGGTTTGCAGCTGCAAGCTATTTAGCGG
ATAAGATTGTGCATGGCACACAAGATGATATCGATCATTACTTGGCCTACCTCAAGTCAGGAAATTCTGATTACCCACTT
GAAGTGATTGCTAAAGCAGGCGTTGATATGGCAAAAGGTGATTATTTGGAAGCTGCCTTTAAAGTCTTTGACGAACGTCT
AACAGAACTTGAAGTACTCGTGTCCAAAGGCATACACAAAAGCTAA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(202-581)

Oligopeptidase F (Peptidase_M3_N)

(112-181)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

71.215

100

0.712

Multiple sequence alignment