Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EKS25_RS01610 Genome accession   NZ_CP063382
Coordinates   297940..299313 (+) Length   457 a.a.
NCBI ID   WP_003723898.1    Uniprot ID   L8DQ47
Organism   Listeria monocytogenes strain clinical isolate of L. monocytogenes isolate 15-01121     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 292940..304313
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EKS25_RS01590 (EKS25_01590) - 293294..293752 (+) 459 WP_003726421.1 CtsR family transcriptional regulator -
  EKS25_RS01595 (EKS25_01595) - 293765..294283 (+) 519 WP_003734530.1 UvrB/UvrC motif-containing protein -
  EKS25_RS01600 (EKS25_01600) - 294280..295302 (+) 1023 WP_003734531.1 protein arginine kinase -
  EKS25_RS01605 (EKS25_01605) - 295331..297793 (+) 2463 WP_003726424.1 ATP-dependent Clp protease ATP-binding subunit -
  EKS25_RS01610 (EKS25_01610) radA 297940..299313 (+) 1374 WP_003723898.1 DNA repair protein RadA Machinery gene
  EKS25_RS01615 (EKS25_01615) - 299446..300519 (+) 1074 WP_003726425.1 PIN/TRAM domain-containing protein -
  EKS25_RS01620 (EKS25_01620) ispD 300539..301237 (+) 699 WP_003734532.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  EKS25_RS01625 (EKS25_01625) ispF 301230..301703 (+) 474 WP_003728083.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  EKS25_RS01630 (EKS25_01630) gltX 301722..303197 (+) 1476 WP_003728082.1 glutamate--tRNA ligase -
  EKS25_RS01635 (EKS25_01635) epsC 303597..304211 (+) 615 WP_003726845.1 serine O-acetyltransferase EpsC -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49991.63 Da        Isoelectric Point: 7.9613

>NTDB_id=494932 EKS25_RS01610 WP_003723898.1 297940..299313(+) (radA) [Listeria monocytogenes strain clinical isolate of L. monocytogenes isolate 15-01121]
MAKAKRTTKFVCQACGYESAKWMGKCPNCNEWNQMVEALEPSKKSRSAFNHTGEPSKATPITQIASETEKRVETNMPELN
RVLGGGVVPGSMVLVGGDPGIGKSTLLLQVSAQLTLTNKKVLYISGEESIKQTKLRAERLQVSGDNLYVYAETNLEAVQE
TIDFVKPDFVVIDSIQTVYHPDVTSAAGSVSQVRECTATLMRIAKMQNIAIFIVGHVTKEGAIAGPRLLEHMVDTVLYFE
GERHHAYRILRAVKNRFGSTNEMGIFEMRDVGLVEVANPSEVFLEERLEGASGSTVVASMEGTRPVLVEIQALVSPTMFG
NAKRMATGIDYNKVSLIMAVLEKRVGLMLQNQDAYLKAAGGVKLDEPAVDLAVAVSVASSYRDKPTRSTDCFIGELGLTG
EIRRVARIEQRVQEAAKLGFKRIFIPKNNEGNWKIPKDVQVVGVETIGEALKKALPD

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=494932 EKS25_RS01610 WP_003723898.1 297940..299313(+) (radA) [Listeria monocytogenes strain clinical isolate of L. monocytogenes isolate 15-01121]
ATGGCTAAAGCAAAAAGGACAACCAAATTTGTGTGTCAGGCATGTGGATATGAATCGGCAAAATGGATGGGAAAATGTCC
GAATTGCAACGAGTGGAATCAGATGGTAGAAGCTTTGGAGCCGTCAAAAAAATCACGCTCAGCTTTTAATCATACGGGAG
AACCTTCGAAAGCGACTCCAATTACTCAAATAGCAAGTGAAACAGAAAAACGTGTCGAAACCAATATGCCGGAGTTAAAT
AGAGTTCTTGGCGGAGGTGTGGTTCCAGGATCTATGGTACTTGTCGGTGGGGATCCTGGTATCGGGAAGTCGACTTTATT
ACTGCAAGTTTCAGCTCAACTGACGCTCACAAATAAAAAAGTATTGTATATATCAGGAGAAGAATCTATCAAACAAACGA
AGCTGCGGGCGGAACGCTTGCAAGTTTCGGGAGATAATTTATATGTGTATGCAGAAACAAATTTAGAAGCAGTTCAAGAA
ACGATTGATTTTGTGAAGCCTGATTTTGTAGTCATTGACTCCATTCAGACTGTTTATCATCCCGATGTTACAAGTGCAGC
GGGGAGTGTTTCGCAAGTTAGGGAATGTACAGCGACATTGATGCGAATTGCTAAAATGCAAAACATTGCAATCTTTATTG
TTGGACATGTAACAAAGGAAGGTGCTATTGCGGGGCCGCGCCTACTTGAACATATGGTTGATACTGTGCTTTATTTTGAA
GGGGAGCGTCATCATGCTTACCGGATTTTGCGTGCAGTGAAAAACCGTTTTGGTTCCACTAATGAAATGGGTATATTTGA
AATGCGAGATGTTGGACTTGTAGAAGTTGCTAATCCTTCCGAAGTGTTCTTGGAAGAACGTCTTGAAGGCGCTTCAGGTT
CAACGGTTGTAGCTTCTATGGAAGGAACTCGCCCCGTCCTTGTGGAAATACAAGCGCTTGTTTCGCCTACGATGTTTGGT
AATGCTAAGCGAATGGCGACAGGAATAGACTATAATAAAGTTTCGCTAATTATGGCTGTTTTAGAAAAGCGAGTAGGTTT
AATGTTGCAAAACCAAGATGCTTATTTGAAAGCAGCTGGTGGGGTTAAATTGGATGAACCTGCAGTGGATTTGGCGGTTG
CAGTCAGTGTTGCATCTAGCTATCGTGATAAACCAACAAGAAGTACGGATTGCTTTATTGGAGAACTTGGACTTACAGGT
GAGATTCGTCGTGTTGCAAGAATTGAACAACGCGTACAAGAAGCCGCAAAACTTGGCTTTAAGCGAATTTTTATTCCTAA
AAATAACGAAGGTAATTGGAAAATACCGAAAGACGTGCAAGTGGTTGGGGTGGAAACGATTGGAGAAGCTTTGAAGAAAG
CTTTACCAGATTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(64-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(10-37)

Subunit ChlI of Mg-chelatase (ChlI)

(363-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB L8DQ47

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

68.421

99.781

0.683
  radA Streptococcus pneumoniae Rx1

65.265

98.906

0.646
  radA Streptococcus pneumoniae D39

65.265

98.906

0.646
  radA Streptococcus pneumoniae R6

65.265

98.906

0.646
  radA Streptococcus pneumoniae TIGR4

65.265

98.906

0.646
  radA Streptococcus mitis NCTC 12261

65.265

98.906

0.646
  radA Streptococcus mitis SK321

65.044

98.906

0.643