Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   EKR40_RS03300 Genome accession   NZ_CP063381
Coordinates   672233..672712 (+) Length   159 a.a.
NCBI ID   WP_031694734.1    Uniprot ID   -
Organism   Listeria monocytogenes strain clinical isolate of L. monocytogenes isolate 12-05460     
Function   ssDNA binding (predicted from homology)   
DNA processing

Genomic Context


Location: 667233..677712
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EKR40_RS03245 (EKR40_03245) - 667683..668039 (+) 357 WP_023552245.1 hypothetical protein -
  EKR40_RS03250 (EKR40_03250) - 668036..668452 (+) 417 WP_023552247.1 hypothetical protein -
  EKR40_RS03255 (EKR40_03255) - 668449..668625 (+) 177 WP_023552249.1 hypothetical protein -
  EKR40_RS03260 (EKR40_03260) - 668640..669359 (+) 720 WP_023552251.1 DUF2786 domain-containing protein -
  EKR40_RS03265 (EKR40_03265) - 669363..669665 (+) 303 WP_023552253.1 hypothetical protein -
  EKR40_RS03270 (EKR40_03270) - 669662..670063 (+) 402 WP_031694732.1 YopX family protein -
  EKR40_RS03275 (EKR40_03275) - 670060..670545 (+) 486 WP_031694733.1 pentapeptide repeat-containing protein -
  EKR40_RS03280 (EKR40_03280) - 670542..671003 (+) 462 WP_023552261.1 hypothetical protein -
  EKR40_RS03285 (EKR40_03285) - 671000..671383 (+) 384 WP_023552263.1 hypothetical protein -
  EKR40_RS03290 (EKR40_03290) - 671404..671652 (+) 249 WP_023552266.1 ASCH/PUA domain-containing protein -
  EKR40_RS03295 (EKR40_03295) - 671652..672233 (+) 582 WP_023552268.1 DUF3310 domain-containing protein -
  EKR40_RS03300 (EKR40_03300) ssbA 672233..672712 (+) 480 WP_031694734.1 single-stranded DNA-binding protein Machinery gene
  EKR40_RS03305 (EKR40_03305) - 672733..672972 (+) 240 WP_023552274.1 hypothetical protein -
  EKR40_RS03310 (EKR40_03310) - 672978..673148 (+) 171 WP_023552276.1 hypothetical protein -
  EKR40_RS03315 (EKR40_03315) - 673219..673737 (+) 519 WP_023552279.1 sigma factor-like helix-turn-helix DNA-binding protein -
  EKR40_RS03320 (EKR40_03320) - 673734..674276 (+) 543 WP_023552281.1 tyrosine-type recombinase/integrase -
  EKR40_RS03325 (EKR40_03325) - 674392..674625 (+) 234 WP_126290679.1 hypothetical protein -
  EKR40_RS03330 (EKR40_03330) - 674896..675222 (+) 327 WP_031694737.1 hypothetical protein -
  EKR40_RS03340 (EKR40_03340) - 675677..676204 (+) 528 WP_012951321.1 phage terminase small subunit P27 family -

Sequence


Protein


Download         Length: 159 a.a.        Molecular weight: 17524.47 Da        Isoelectric Point: 5.2652

>NTDB_id=494894 EKR40_RS03300 WP_031694734.1 672233..672712(+) (ssbA) [Listeria monocytogenes strain clinical isolate of L. monocytogenes isolate 12-05460]
MMNRVVLVGRLTKDPDLRYTPAGAAVATFTLAVNRPFKNAQGEQEADFINCVVWRKPAENVANFLKKGSMAGVDGRVQTR
NYEDNDGKRVFVTEVVAESVQFLEPKNNQGRASTNDYQNKANYSNNIQTSSYAADASQKGGAFVNDSKPIDIPDDDLPF

Nucleotide


Download         Length: 480 bp        

>NTDB_id=494894 EKR40_RS03300 WP_031694734.1 672233..672712(+) (ssbA) [Listeria monocytogenes strain clinical isolate of L. monocytogenes isolate 12-05460]
ATGATGAATCGTGTAGTGCTTGTAGGTCGATTAACTAAAGACCCTGATTTACGATATACGCCAGCAGGCGCGGCTGTTGC
GACTTTTACATTAGCTGTAAATCGCCCATTTAAAAATGCACAAGGAGAACAAGAAGCCGATTTCATTAATTGTGTTGTTT
GGCGAAAACCAGCAGAAAACGTTGCTAATTTCTTGAAGAAAGGAAGCATGGCGGGCGTTGACGGTCGCGTACAGACTCGA
AATTACGAAGATAACGACGGTAAACGTGTTTTCGTTACAGAAGTAGTAGCTGAATCAGTTCAATTCTTAGAACCTAAAAA
CAACCAAGGAAGAGCTTCAACAAATGATTATCAAAACAAAGCTAATTATTCAAACAACATCCAAACAAGCTCATATGCAG
CGGATGCGAGTCAGAAAGGCGGTGCATTTGTTAATGATAGCAAGCCAATCGATATTCCAGATGATGATTTACCGTTTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

61.798

100

0.692

  ssb Latilactobacillus sakei subsp. sakei 23K

54.335

100

0.591

  ssbB Bacillus subtilis subsp. subtilis str. 168

66.038

66.667

0.44

  ssb Neisseria meningitidis MC58

33.333

100

0.365