Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   KIM15_RS27795 Genome accession   NZ_CP074890
Coordinates   5378575..5380071 (-) Length   498 a.a.
NCBI ID   WP_001040651.1    Uniprot ID   -
Organism   Escherichia coli strain 2009C_3996     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5373575..5385071
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIM15_RS27770 (KIM15_27400) panM 5373798..5374181 (+) 384 WP_000778789.1 aspartate 1-decarboxylase autocleavage activator PanM -
  KIM15_RS27775 (KIM15_27405) livJ 5374380..5375483 (-) 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  KIM15_RS27780 (KIM15_27410) rpoH 5375754..5376608 (-) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  KIM15_RS27785 (KIM15_27415) ftsX 5376853..5377911 (-) 1059 WP_001042001.1 permease-like cell division protein FtsX -
  KIM15_RS27790 (KIM15_27420) ftsE 5377904..5378572 (-) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  KIM15_RS27795 (KIM15_27425) pilA 5378575..5380071 (-) 1497 WP_001040651.1 signal recognition particle-docking protein FtsY Machinery gene
  KIM15_RS27800 (KIM15_27430) rsmD 5380221..5380817 (+) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  KIM15_RS27805 (KIM15_27435) yhhL 5380807..5381076 (+) 270 WP_001295207.1 DUF1145 family protein -
  KIM15_RS27810 (KIM15_27440) yhhM 5381079..5381438 (-) 360 WP_000042873.1 DUF2500 domain-containing protein -
  KIM15_RS27815 (KIM15_27445) yhhN 5381579..5382205 (+) 627 WP_000964718.1 lysoplasmalogenase -
  KIM15_RS27820 (KIM15_27450) zntA 5382279..5384477 (+) 2199 WP_000106580.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  KIM15_RS27825 (KIM15_27455) tusA 5384579..5384824 (-) 246 WP_000130621.1 sulfurtransferase TusA -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54671.46 Da        Isoelectric Point: 4.1468

>NTDB_id=494762 KIM15_RS27795 WP_001040651.1 5378575..5380071(-) (pilA) [Escherichia coli strain 2009C_3996]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVAEETPEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=494762 KIM15_RS27795 WP_001040651.1 5378575..5380071(-) (pilA) [Escherichia coli strain 2009C_3996]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCGGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGCAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCAGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCAGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTAGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATTGCCCAGGA
GCAAGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACTACGACGATTGGTAAGCTGGCACGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCAGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCAGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGTACCGGGCAGAA
TGCGGTAAGCCAGGCCAAACTGTTCCACGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378