Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   AWH56_RS07860 Genome accession   NZ_CP063356
Coordinates   1595762..1597135 (+) Length   457 a.a.
NCBI ID   WP_071315244.1    Uniprot ID   A0A1S2MFC3
Organism   Anaerobacillus isosaccharinicus strain NB2006     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1590762..1602135
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AWH56_RS07840 (AWH56_007840) - 1591008..1591475 (+) 468 WP_071315240.1 CtsR family transcriptional regulator -
  AWH56_RS07845 (AWH56_007845) - 1591500..1592036 (+) 537 WP_071315241.1 UvrB/UvrC motif-containing protein -
  AWH56_RS07850 (AWH56_007850) - 1592036..1593112 (+) 1077 WP_071315242.1 protein arginine kinase -
  AWH56_RS07855 (AWH56_007855) clpC 1593133..1595577 (+) 2445 WP_071315243.1 ATP-dependent protease ATP-binding subunit ClpC -
  AWH56_RS07860 (AWH56_007860) radA 1595762..1597135 (+) 1374 WP_071315244.1 DNA repair protein RadA Machinery gene
  AWH56_RS07865 (AWH56_007865) disA 1597140..1598213 (+) 1074 WP_071315245.1 DNA integrity scanning diadenylate cyclase DisA -
  AWH56_RS07870 (AWH56_007870) - 1598405..1599496 (+) 1092 WP_071315246.1 PIN/TRAM domain-containing protein -
  AWH56_RS07875 (AWH56_007875) ispD 1599781..1600473 (+) 693 WP_071315247.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  AWH56_RS07880 (AWH56_007880) ispF 1600470..1600946 (+) 477 WP_071315248.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49629.17 Da        Isoelectric Point: 6.5116

>NTDB_id=494586 AWH56_RS07860 WP_071315244.1 1595762..1597135(+) (radA) [Anaerobacillus isosaccharinicus strain NB2006]
MAKRKTKFVCQECGYESTKWMGKCPGCQSWNSMVEELEETSTQKRGFVTSGGSVNKKPQPITQIERDLEQRLSTNMAELN
RVLGGGIVPGSLVLVGGDPGIGKSTLLLQVSAKLANNNHKVLYISGEESVKQTKLRADRLDITSDNLFVLAETDLEFIEK
AIEDLNPALVIIDSIQTVYMAEVTSAPGSVSQVRESTAALMRIAKTKGTAIFIVGHVTKQGAIAGPRLLEHMVDCVLYFE
GERHHTYRILRAVKNRFGSTNEIGIFEMKEIGLEEVLNPSEIFLEERSQGASGSIVVASMEGTRTVLVELQALVSPTTFG
NPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLNVAGGVRLDEPAVDLGIAISIASSFRDFPTSPTDVAIGEIGLTG
EVRRVSRIEQRVIEAAKLGFKRVIIPEKNLGGWNIPNGISVVGVATVKEALDILMGG

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=494586 AWH56_RS07860 WP_071315244.1 1595762..1597135(+) (radA) [Anaerobacillus isosaccharinicus strain NB2006]
ATGGCGAAACGTAAAACGAAGTTTGTTTGTCAAGAATGCGGGTATGAATCAACAAAGTGGATGGGAAAATGCCCTGGGTG
TCAGAGTTGGAACTCCATGGTTGAGGAATTAGAAGAAACAAGTACTCAAAAAAGAGGTTTTGTTACTTCCGGTGGTTCTG
TTAATAAAAAGCCACAGCCAATTACTCAGATCGAAAGAGATTTAGAACAAAGACTTAGTACAAATATGGCGGAACTTAAT
CGAGTATTAGGTGGCGGAATTGTGCCAGGTTCATTAGTTCTTGTTGGTGGAGATCCTGGAATCGGGAAGTCTACATTGCT
TTTACAAGTTTCAGCGAAATTAGCTAATAATAATCATAAGGTTTTATATATTTCAGGTGAAGAATCTGTTAAGCAAACAA
AGTTACGTGCAGATAGACTAGATATCACATCTGATAATTTATTTGTTTTAGCAGAAACTGATTTAGAATTTATTGAAAAA
GCAATTGAGGACTTAAATCCTGCCTTAGTGATCATTGATTCTATTCAAACGGTTTATATGGCCGAAGTTACTTCAGCACC
AGGAAGTGTATCCCAAGTAAGAGAAAGTACTGCGGCGTTAATGAGAATTGCCAAAACAAAAGGGACTGCAATTTTTATAG
TTGGACATGTAACAAAACAAGGGGCCATAGCCGGTCCTAGATTACTTGAACATATGGTTGATTGTGTTCTTTACTTTGAA
GGAGAGCGTCATCACACTTATCGAATTCTTCGAGCAGTCAAAAATCGCTTCGGATCAACGAATGAAATAGGGATTTTTGA
AATGAAGGAAATAGGACTAGAAGAAGTATTAAATCCTTCTGAGATCTTTTTAGAGGAACGTTCTCAAGGAGCATCTGGTT
CTATCGTAGTTGCTTCCATGGAAGGAACAAGAACTGTTTTAGTCGAACTGCAAGCCTTAGTTTCGCCAACGACGTTTGGG
AACCCAAGACGAATGGCAACGGGAATTGATCATAATCGAGTTTCGTTATTAATGGCAGTATTAGAAAAGCGTGTCGGGCT
CCTTTTGCAGAACCAGGATGCATATTTAAATGTTGCTGGTGGTGTACGTTTAGATGAGCCTGCTGTAGATTTAGGAATAG
CGATTAGTATTGCTTCTAGCTTCCGTGATTTTCCAACAAGTCCAACAGATGTGGCTATTGGAGAAATTGGTTTAACAGGA
GAGGTGCGTCGAGTTTCACGGATTGAGCAACGTGTGATTGAAGCTGCCAAATTAGGATTTAAAAGAGTCATTATCCCGGA
AAAAAATCTTGGAGGCTGGAATATCCCTAATGGAATTTCTGTAGTTGGAGTTGCAACGGTGAAAGAGGCATTAGACATCT
TGATGGGAGGTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(66-221)

Subunit ChlI of Mg-chelatase (ChlI)

(349-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1S2MFC3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

72.926

100

0.731
  radA Streptococcus pneumoniae Rx1

60.089

98.687

0.593
  radA Streptococcus pneumoniae D39

60.089

98.687

0.593
  radA Streptococcus pneumoniae R6

60.089

98.687

0.593
  radA Streptococcus pneumoniae TIGR4

60.089

98.687

0.593
  radA Streptococcus mitis NCTC 12261

60.089

98.687

0.593
  radA Streptococcus mitis SK321

60.089

98.687

0.593