Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   IHJ54_RS02355 Genome accession   NZ_CP063241
Coordinates   540017..541399 (-) Length   460 a.a.
NCBI ID   WP_014914021.1    Uniprot ID   A0A3S0ZXC5
Organism   Pectobacterium brasiliense strain ZLMLSHJ5     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 535017..546399
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHJ54_RS02325 (IHJ54_02325) pepP 535211..536536 (-) 1326 WP_014914017.1 Xaa-Pro aminopeptidase -
  IHJ54_RS02330 (IHJ54_02330) - 536594..537181 (-) 588 WP_039548009.1 YecA family protein -
  IHJ54_RS02335 (IHJ54_02335) zapA 537374..537703 (+) 330 WP_010284667.1 cell division protein ZapA -
  IHJ54_RS02345 (IHJ54_02345) - 538001..538648 (+) 648 WP_203449918.1 5-formyltetrahydrofolate cyclo-ligase -
  IHJ54_RS02350 (IHJ54_02350) nadR 538645..539898 (-) 1254 WP_039279404.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  IHJ54_RS02355 (IHJ54_02355) radA 540017..541399 (-) 1383 WP_014914021.1 DNA repair protein RadA Machinery gene
  IHJ54_RS02360 (IHJ54_02360) serB 541417..542394 (-) 978 WP_039510182.1 phosphoserine phosphatase -
  IHJ54_RS02365 (IHJ54_02365) - 542551..543249 (+) 699 WP_039504966.1 YtjB family periplasmic protein -
  IHJ54_RS02370 (IHJ54_02370) - 543298..544218 (-) 921 WP_039504967.1 hypothetical protein -
  IHJ54_RS02375 (IHJ54_02375) prfC 544510..546099 (+) 1590 WP_039510188.1 peptide chain release factor 3 -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49620.19 Da        Isoelectric Point: 7.4218

>NTDB_id=494089 IHJ54_RS02355 WP_014914021.1 540017..541399(-) (radA) [Pectobacterium brasiliense strain ZLMLSHJ5]
MAKAVKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLASASTSRSDRLTGYAGESAGVSRVQKLSEISLEALPRFSTG
FQEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLSENMKTLYVTGEESLQQVAMRAHRLNLPTQNLNMLSETSIE
QICLIAEQEQPKLMVIDSIQVMHLADIQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREISNPSAIFLSRGDEITSGSSVMVVWEGTRPLLVEIQALVDQ
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALLLSLVSSFRDRPLPQDLVIFGEV
GLAGEIRPVPSGQERITEAAKHGFKRAIVPHANMPKKVPASMQVFGVKKLADALAILDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=494089 IHJ54_RS02355 WP_014914021.1 540017..541399(-) (radA) [Pectobacterium brasiliense strain ZLMLSHJ5]
GTGGCAAAAGCCGTCAAACGGGCGTTTGTATGTAATGAATGCGGGGCTGACTACCCGCGCTGGCAAGGGCAGTGCAGCGC
CTGCCATGCCTGGAATACCATTACCGAAGTCCGTCTGGCCTCGGCGTCAACATCACGTTCCGACCGTCTCACCGGCTATG
CGGGCGAAAGCGCTGGCGTCAGCCGGGTACAAAAGCTCTCGGAAATCAGTCTTGAAGCCCTACCGCGCTTTTCCACCGGC
TTTCAGGAGTTTGACCGCGTTCTGGGCGGCGGCGTCGTCCCCGGCAGCGCGATTCTGATCGGCGGTAACCCCGGCGCGGG
TAAAAGTACCTTACTGCTGCAAACGCTCTGCAAGCTGTCAGAGAACATGAAAACCCTGTACGTCACTGGGGAAGAATCCT
TGCAGCAAGTGGCGATGCGGGCGCATCGTCTCAACCTACCGACCCAAAATCTCAATATGCTGTCGGAAACCAGCATCGAA
CAGATTTGCCTGATTGCCGAGCAGGAACAGCCGAAGCTGATGGTGATCGACTCCATTCAGGTGATGCATCTCGCGGATAT
TCAATCGTCACCCGGTAGCGTAGCGCAGGTGCGTGAAACCGCCGCCTACCTGACGCGCTTCGCCAAAACACGCGGCGTCG
CTATCGTGATGGTCGGCCACGTCACCAAAGACGGTTCGCTCGCCGGGCCGAAAGTGTTGGAACACTGCATCGACTGCTCC
GTACTGCTGGATGGCGATGCCGATTCGCGCTTCCGCACCCTGCGCAGCCATAAAAACCGCTTCGGTGCCGTTAACGAGCT
GGGCGTGTTCGCGATGACGGAACAGGGTCTACGCGAGATCAGCAATCCGTCGGCAATTTTCCTCAGTCGCGGTGACGAAA
TCACGTCCGGCAGTTCGGTCATGGTGGTGTGGGAAGGTACGCGTCCGCTGCTGGTCGAAATTCAGGCGCTGGTGGATCAA
TCGATGATGGCTAACCCGCGTCGCGTGGCGGTCGGGCTGGAGCAAAATCGCCTGGCGATCCTGCTGGCGGTATTACATCG
CCACGGCGGCTTGCAGATGTCCGATCAGGATGTGTTCGTGAACGTTGTCGGCGGCGTTAAAGTCACCGAAACCAGCGCCG
ACCTGGCGCTGCTGCTATCGCTGGTCTCCAGCTTCCGCGACCGCCCGTTGCCGCAAGATCTTGTCATCTTCGGTGAAGTC
GGTCTGGCGGGTGAAATCCGCCCGGTTCCCAGCGGCCAAGAGCGGATTACCGAAGCCGCTAAGCATGGCTTCAAACGCGC
CATCGTTCCTCATGCCAATATGCCGAAGAAAGTCCCTGCCAGCATGCAGGTGTTCGGCGTGAAAAAGCTGGCCGACGCGC
TGGCGATCCTCGACGATCTCTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(89-225)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S0ZXC5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.261

100

0.483
  radA Streptococcus pneumoniae Rx1

42.982

99.13

0.426
  radA Streptococcus pneumoniae D39

42.982

99.13

0.426
  radA Streptococcus pneumoniae R6

42.982

99.13

0.426
  radA Streptococcus pneumoniae TIGR4

42.982

99.13

0.426
  radA Streptococcus mitis NCTC 12261

42.982

99.13

0.426
  radA Streptococcus mitis SK321

42.982

99.13

0.426