Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   IM720_RS27895 Genome accession   NZ_CP063233
Coordinates   6200145..6201512 (-) Length   455 a.a.
NCBI ID   WP_069077072.1    Uniprot ID   A0AB36Y2N8
Organism   Pseudomonas fluorescens strain KF1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 6195145..6206512
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IM720_RS27870 (IM720_27870) yjiA 6195482..6196441 (-) 960 WP_024077498.1 GTPase -
  IM720_RS27875 (IM720_27875) - 6196577..6196774 (-) 198 WP_003176208.1 YbdD/YjiX family protein -
  IM720_RS27880 (IM720_27880) - 6196790..6198856 (-) 2067 WP_024077499.1 carbon starvation CstA family protein -
  IM720_RS27885 (IM720_27885) - 6199030..6199398 (+) 369 WP_150295803.1 PilZ domain-containing protein -
  IM720_RS27890 (IM720_27890) - 6199403..6200128 (-) 726 WP_168233288.1 hypothetical protein -
  IM720_RS27895 (IM720_27895) radA 6200145..6201512 (-) 1368 WP_069077072.1 DNA repair protein RadA Machinery gene
  IM720_RS27900 (IM720_27900) - 6201548..6202087 (-) 540 WP_168233287.1 ankyrin repeat domain-containing protein -
  IM720_RS27905 (IM720_27905) katB 6202142..6203683 (-) 1542 WP_024077504.1 catalase KatB -
  IM720_RS27910 (IM720_27910) mscL 6203971..6204387 (+) 417 WP_017737414.1 large-conductance mechanosensitive channel protein MscL -
  IM720_RS27915 (IM720_27915) - 6204432..6205208 (-) 777 WP_193689862.1 ferredoxin--NADP reductase -
  IM720_RS27920 (IM720_27920) - 6205614..6206324 (+) 711 WP_024077506.1 helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48759.23 Da        Isoelectric Point: 6.8987

>NTDB_id=494011 IM720_RS27895 WP_069077072.1 6200145..6201512(-) (radA) [Pseudomonas fluorescens strain KF1]
MAKAKRMYGCTECGATFPKWAGQCTECGAWNTLTETMIESGGASAPTGRAGWTGQQAQIKTLAEVSVEEIPRFSTASGEL
DRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCSIASRMPALYVTGEESQQQVAMRARRLGLPQDQLRVMTETCIESIIA
TARIEKPKVMVIDSIQTIFTEQLQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFAMTDRGLKEVSNPSAIFLTRAQEEVPGSVVMATWEGTRPMLVEVQALVDDSHLA
NPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSG
EVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEAPPGLQVIGVTRLEQALDALFE

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=494011 IM720_RS27895 WP_069077072.1 6200145..6201512(-) (radA) [Pseudomonas fluorescens strain KF1]
ATGGCAAAGGCCAAGCGCATGTACGGCTGCACGGAGTGCGGCGCGACCTTTCCCAAGTGGGCCGGCCAATGCACCGAGTG
CGGTGCGTGGAATACCCTCACGGAAACCATGATCGAAAGCGGCGGCGCCTCGGCCCCTACCGGCCGCGCCGGCTGGACCG
GGCAACAGGCGCAGATCAAGACCCTGGCTGAAGTCAGTGTCGAAGAGATTCCGCGCTTCTCCACCGCATCCGGCGAGCTG
GACCGTGTGCTCGGCGGCGGCCTGGTGGATGGTTCGGTGGTGCTGATCGGCGGCGACCCGGGCATCGGCAAGTCGACGAT
CCTGCTGCAAACCCTGTGCAGCATCGCCAGCCGCATGCCGGCGCTGTACGTCACGGGTGAAGAATCCCAGCAGCAAGTGG
CCATGCGCGCCCGTCGTTTGGGCCTGCCCCAGGATCAACTGCGGGTCATGACGGAAACCTGCATCGAAAGCATCATCGCC
ACGGCCCGCATCGAAAAACCCAAGGTCATGGTGATCGACTCGATCCAGACGATTTTCACCGAACAACTGCAATCGGCGCC
GGGTGGCGTGTCCCAGGTGCGCGAAAGTGCGGCGCTGCTGGTGCGTTATGCCAAGCAGAGTGGCACGGCGATCTTCCTGG
TTGGCCACGTGACCAAGGAAGGTGCATTGGCCGGGCCACGCGTGCTGGAGCATATGGTCGACACCGTGCTGTATTTCGAA
GGCGAATCCGATGGCCGCCTGCGTTTGCTGCGCGCGGTGAAGAACCGCTTTGGCGCGGTGAACGAGCTGGGTGTGTTCGC
CATGACCGACCGGGGGCTGAAAGAAGTCTCCAATCCATCGGCGATTTTTCTCACCCGTGCCCAGGAAGAAGTCCCCGGCA
GTGTGGTGATGGCGACGTGGGAAGGCACTCGGCCGATGCTGGTGGAAGTCCAGGCGCTGGTGGATGACAGCCATTTGGCC
AACCCCCGTCGAGTCACCCTGGGCCTGGATCAGAACCGCCTGGCGATGTTGCTGGCGGTATTGCACCGCCATGGTGGCAT
TCCGACCCATGACCAAGACGTGTTCCTCAACGTGGTGGGCGGGGTCAAGGTGCTGGAAACCGCATCCGACCTGGCGTTGA
TGGCGGCGGTGATGTCCAGCCTGCGCAACCGGCCGCTGCCCCATGACTTGCTGGTGTTTGGCGAAGTCGGCCTGTCGGGC
GAAGTGCGCCCGGTGCCCAGTGGTCAGGAGCGCCTCAAGGAAGCGGCCAAGCATGGCTTCAAACGCGCGATTGTGCCGAA
GGGCAATGCGCCGAAGGAAGCGCCGCCAGGGTTGCAGGTGATCGGGGTAACGCGCCTGGAACAGGCATTGGATGCACTTT
TCGAATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(80-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(353-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.672

100

0.499
  radA Streptococcus pneumoniae Rx1

46.957

100

0.475
  radA Streptococcus pneumoniae R6

46.957

100

0.475
  radA Streptococcus pneumoniae TIGR4

46.957

100

0.475
  radA Streptococcus pneumoniae D39

46.957

100

0.475
  radA Streptococcus mitis SK321

47.149

100

0.473
  radA Streptococcus mitis NCTC 12261

46.93

100

0.47