Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   KIM27_RS25560 Genome accession   NZ_CP074849
Coordinates   5059616..5060380 (+) Length   254 a.a.
NCBI ID   WP_001136229.1    Uniprot ID   B7L5T2
Organism   Escherichia coli strain 7_12_15B     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 5054616..5065380
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIM27_RS25540 (KIM27_25275) acpT 5055570..5056157 (+) 588 WP_000285771.1 4'-phosphopantetheinyl transferase AcpT -
  KIM27_RS25545 (KIM27_25280) nikA 5056268..5057842 (+) 1575 WP_000953353.1 nickel ABC transporter substrate-binding protein -
  KIM27_RS25550 (KIM27_25285) nikB 5057842..5058786 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  KIM27_RS25555 (KIM27_25290) nikC 5058783..5059616 (+) 834 WP_001008968.1 nickel ABC transporter permease subunit NikC -
  KIM27_RS25560 (KIM27_25295) amiE 5059616..5060380 (+) 765 WP_001136229.1 nickel import ATP-binding protein NikD Regulator
  KIM27_RS25565 (KIM27_25300) nikE 5060377..5061183 (+) 807 WP_000173630.1 nickel import ATP-binding protein NikE -
  KIM27_RS25570 (KIM27_25305) nikR 5061189..5061590 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26820.38 Da        Isoelectric Point: 6.3761

>NTDB_id=493733 KIM27_RS25560 WP_001136229.1 5059616..5060380(+) (amiE) [Escherichia coli strain 7_12_15B]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=493733 KIM27_RS25560 WP_001136229.1 5059616..5060380(+) (amiE) [Escherichia coli strain 7_12_15B]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACAGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7L5T2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398