Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   IMY50_RS25720 Genome accession   NZ_CP063158
Coordinates   5022213..5023562 (-) Length   449 a.a.
NCBI ID   WP_071713847.1    Uniprot ID   -
Organism   Bacillus cereus strain CF4-51     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5017213..5028562
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IMY50_RS25685 (IMY50_25680) - 5017376..5018071 (-) 696 WP_071713842.1 hypothetical protein -
  IMY50_RS25690 (IMY50_25685) - 5018058..5018846 (-) 789 WP_071713843.1 hypothetical protein -
  IMY50_RS25695 (IMY50_25690) - 5018878..5019447 (-) 570 WP_001211060.1 hypothetical protein -
  IMY50_RS25700 (IMY50_25695) - 5019565..5020047 (-) 483 WP_000023374.1 hypothetical protein -
  IMY50_RS25705 (IMY50_25700) raiA 5020319..5020861 (-) 543 WP_000671193.1 ribosome-associated translation inhibitor RaiA -
  IMY50_RS25710 (IMY50_25705) cspC 5021185..5021382 (-) 198 WP_001990088.1 cold shock protein CspC -
  IMY50_RS25715 (IMY50_25710) - 5021509..5022213 (-) 705 WP_071713846.1 ComF family protein -
  IMY50_RS25720 (IMY50_25715) comFA 5022213..5023562 (-) 1350 WP_071713847.1 ATP-dependent helicase ComFA Machinery gene
  IMY50_RS25725 (IMY50_25720) - 5023691..5025106 (-) 1416 WP_071713848.1 C40 family peptidase -
  IMY50_RS25730 (IMY50_25725) - 5025255..5025569 (-) 315 WP_000400857.1 helix-turn-helix domain-containing protein -
  IMY50_RS25735 (IMY50_25730) - 5025731..5026573 (-) 843 WP_000684727.1 DegV family protein -
  IMY50_RS25740 (IMY50_25735) - 5026810..5027445 (+) 636 WP_071713849.1 YigZ family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 50947.77 Da        Isoelectric Point: 9.9290

>NTDB_id=493656 IMY50_RS25720 WP_071713847.1 5022213..5023562(-) (comFA) [Bacillus cereus strain CF4-51]
MLPGKQLLLDELSSDLQRELNDLKKKGEVVCVQGVKKKASKDVCQRCGNIEQRLFASFLCKRCSKVCTYCRKCITMGRVS
ECAVLVRGIAERKREKNLNLLQWDGKLSTGQNLAAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGINEALQKGERVCIAT
PRTDVVLELAPRLQEVFPYIKVAALYGGSVDKEKDAVLVVATTHQLLRYYRAFHVMVVDEIDAFPYCADQMLQYAVKQAM
KERAARIYLTATPDETWKRKVRRGEQKGVIVSGRYHRHPLPVPLFCWCGNWKKSLIHKRIPRVLLQWLQTYLNKKYPIFL
FVPHVRYIEEISLLLKSLNKRIEGVHAEDPGRKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEAPYGGVVYFHYGKTEAMVRAKKHIQGMNKNAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=493656 IMY50_RS25720 WP_071713847.1 5022213..5023562(-) (comFA) [Bacillus cereus strain CF4-51]
ATGCTACCGGGGAAACAGTTGCTATTAGACGAACTTTCTTCAGATTTACAGAGGGAATTAAATGATTTGAAAAAGAAGGG
AGAGGTCGTGTGTGTACAAGGTGTAAAAAAGAAGGCTTCTAAAGATGTATGTCAGCGTTGTGGAAATATAGAACAGCGGC
TATTTGCGTCGTTTTTATGTAAAAGGTGCAGTAAAGTGTGCACATATTGCCGGAAGTGTATAACGATGGGGAGGGTAAGT
GAATGTGCTGTACTTGTTCGCGGGATTGCTGAAAGAAAGAGAGAAAAGAATTTAAATTTGTTACAGTGGGACGGGAAGTT
GTCTACTGGTCAGAATCTGGCGGCACAAGGAGTTATAGAGGCTATTAAGCAAAAAGAATCATTTTTTATTTGGGCTGTAT
GCGGGGCTGGGAAAACAGAGATGTTGTTTTACGGTATTAACGAAGCGCTTCAAAAAGGAGAAAGAGTTTGTATCGCAACG
CCGAGAACGGATGTTGTTCTGGAATTAGCACCGAGATTACAAGAAGTATTTCCATATATAAAGGTAGCGGCTTTATATGG
AGGGAGTGTGGATAAAGAAAAAGATGCAGTACTAGTCGTTGCAACCACGCATCAATTATTGCGTTACTATAGGGCGTTTC
ATGTCATGGTTGTAGATGAGATAGATGCTTTTCCATATTGTGCAGATCAAATGTTACAGTACGCGGTAAAACAAGCGATG
AAAGAAAGAGCGGCGCGTATTTATTTAACTGCGACTCCAGATGAAACGTGGAAGCGAAAAGTTAGAAGAGGTGAACAAAA
AGGTGTTATTGTTTCTGGACGATATCACCGTCATCCTTTGCCAGTTCCTTTATTTTGTTGGTGCGGGAATTGGAAAAAAA
GCCTCATTCATAAAAGAATTCCTCGAGTTTTACTACAGTGGTTACAAACGTACTTAAATAAAAAATATCCTATTTTTTTA
TTTGTTCCCCATGTGCGATATATAGAAGAGATAAGCCTGTTGTTAAAGTCATTAAACAAGCGAATTGAAGGTGTACATGC
AGAAGATCCGGGTAGAAAAGAAAAAGTAGCGGCTTTCAGAAAAGGAGAAATCCCATTATTAGTTACAACGACAATTTTAG
AGCGAGGCGTAACAGTAAAAAATTTGCAAGTAGCGGTTTTAGGGGCGGAAGAAGAAATATTCTCAGAAAGTGCACTCGTA
CAAATTGCGGGCCGAGCAGGGCGGAGCTTTGAAGCACCGTATGGAGGGGTCGTTTATTTTCACTATGGTAAGACAGAGGC
GATGGTGCGCGCGAAAAAACATATTCAAGGTATGAATAAAAATGCGAAAGAACAAGGATTGATCGATTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(312-408)

Type III restriction enzyme, res subunit (ResIII)

(107-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

50.905

98.441

0.501
  comFA Latilactobacillus sakei subsp. sakei 23K

41.709

88.641

0.37