Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   IMY50_RS19930 Genome accession   NZ_CP063158
Coordinates   3952048..3952482 (-) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus cereus strain CF4-51     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3947048..3957482
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IMY50_RS19915 (IMY50_19910) - 3948226..3948783 (+) 558 WP_000062063.1 PadR family transcriptional regulator -
  IMY50_RS19920 (IMY50_19915) metE 3949295..3951583 (+) 2289 WP_061659893.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  IMY50_RS19925 (IMY50_19920) - 3951622..3952011 (-) 390 WP_071713523.1 competence protein ComJ -
  IMY50_RS19930 (IMY50_19925) nucA/comI 3952048..3952482 (-) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  IMY50_RS19935 (IMY50_19930) - 3952709..3953458 (+) 750 WP_000388467.1 DUF3967 domain-containing protein -
  IMY50_RS19940 (IMY50_19935) kinB 3953497..3954771 (-) 1275 WP_000420372.1 sporulation sensor histidine kinase KinB -
  IMY50_RS19945 (IMY50_19940) - 3955082..3955282 (+) 201 WP_000929276.1 hypothetical protein -
  IMY50_RS19950 (IMY50_19945) - 3955405..3956568 (+) 1164 WP_000434552.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=493642 IMY50_RS19930 WP_000811494.1 3952048..3952482(-) (nucA/comI) [Bacillus cereus strain CF4-51]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=493642 IMY50_RS19930 WP_000811494.1 3952048..3952482(-) (nucA/comI) [Bacillus cereus strain CF4-51]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCGTATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGGGGAGCAGGCTCTTGGGTAGGGAATAAGTTAGATAAATATC
CAGATGGTACGCGTGTGAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549