Detailed information NTG0493622

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: IMY50_RS05750
• Genome accession: NZ_CP063158
• Coordinates: 1118112..1121837 (+)
• Length: 1241 a.a.
• NCBI ID: WP_071714134.1
• Uniprot ID: -
• Organism: Bacillus cereus strain CF4-51
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1113112..1126837

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IMY50_RS05735 (IMY50_05735)	-	1113270..1113863 (+)	594	WP_071714132.1	TVP38/TMEM64 family protein	-
IMY50_RS05740 (IMY50_05740)	lepB	1113920..1114483 (+)	564	WP_000751897.1	signal peptidase I	-
IMY50_RS05745 (IMY50_05745)	addB	1114600..1118115 (+)	3516	WP_216115397.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
IMY50_RS05750 (IMY50_05750)	addA	1118112..1121837 (+)	3726	WP_071714134.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
IMY50_RS05755 (IMY50_05755)	-	1121850..1122137 (+)	288	WP_071714135.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
IMY50_RS05760 (IMY50_05760)	gerPF	1122274..1122489 (-)	216	WP_001141566.1	spore germination protein GerPF	-
IMY50_RS05765 (IMY50_05765)	-	1122532..1122918 (-)	387	WP_016513142.1	spore germination protein GerPE	-
IMY50_RS05770 (IMY50_05770)	gerPD	1122934..1123128 (-)	195	WP_001052807.1	spore germination protein GerPD	-
IMY50_RS05775 (IMY50_05775)	gerPC	1123135..1123749 (-)	615	WP_001070763.1	spore germination protein GerPC	-
IMY50_RS05780 (IMY50_05780)	gerPB	1123817..1124023 (-)	207	WP_001012508.1	spore germination protein GerPB	-
IMY50_RS05785 (IMY50_05785)	gerPA	1124038..1124259 (-)	222	WP_001111187.1	spore germination protein GerPA	-
IMY50_RS05790 (IMY50_05790)	-	1124356..1124535 (-)	180	WP_000462857.1	aspartyl-phosphate phosphatase Spo0E family protein	-
IMY50_RS05795 (IMY50_05795)	-	1124782..1125681 (+)	900	WP_001213069.1	fumarylacetoacetate hydrolase family protein	-
IMY50_RS05800 (IMY50_05800)	-	1125716..1125997 (-)	282	WP_000926859.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142793.99 Da
• Isoelectric Point: 4.8163

>NTDB_id=493622 IMY50_RS05750 WP_071714134.1 1118112..1121837(+) (addA) [Bacillus cereus strain CF4-51]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRIETLQADVALLGTLSSAARESWTRLYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSENGEMNPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGATYPEGEDVAAELLCIQQTEEEVMDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEDLATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDATKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPDEIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEDATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITAETLQEQIAGMINKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSEETILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFEQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVNIEE

Nucleotide

• Length: 3726 bp

>NTDB_id=493622 IMY50_RS05750 WP_071714134.1 1118112..1121837(+) (addA) [Bacillus cereus strain CF4-51]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCTGTTGTAGCGAACGGACG
TGATATTTTAGTTGCGGCTGCAGCTGGATCAGGTAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATTAATG
AAGAGAACCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCAGCGCAAGAGATGAAAAACCGAATC
GGGGAAGCGTTAGAAAAGGTATTAATTGATGAACCAGGCTCTCAACACGTAAGAAAGCAGCTGAGCCTATTAAATAAAGC
TTCCATTTCAACAATTCACTCATTCTGTTTACAAGTAATTAGAGGATATTACTACATGCTTGATGTTGATCCTCGTTTCC
GTATTGCGAATCAAACAGAAAATGAATTGTTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTATGGCATCGAA
GATAATACGATATTCTTTGAACTCGTTGATCGTTATACGAGTGACCGTAGTGATGATGACTTACAACGTATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTTGATAAATTAGTAGAAGCATATGACGTAGAAGGAA
AGACAATTGAGGATTTAGTATATGCTTCTTATTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCGGAACAGCATATT
CGTAAGGCAACTGAACTCGCAATGCTTCCTGACGGTCCAGCGCCTCGCATTGAAACCCTGCAAGCAGATGTAGCTTTACT
TGGAACGCTATCATCAGCTGCTCGTGAGTCGTGGACAAGATTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAATGAAGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAGGAAGAGTTATTTAGCCGCAAACCGGAAAGTTTCTTACGAGATTTTCAAGATATGCACCCAGTATTAGA
AAAGCTTGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGAATGGTCGATTTTACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAAATGGTGAAATGAATCCGTCAGCAGTGGCGCTCCAA
TATCGTAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGACACGAACTTTGTACAGGAATCCATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTTGGTGACGTAAAGCAGTCGATTTATCGTTTCCGACTAGCCG
AGCCAGGACTATTCTTAGGAAAATATAAACGCTTCACACAAGAAGGATTGGGCGGCGGAATGAAGATTGACTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTACTAGCAGGTACGAACTTTATTTTTAAACAAATTATGGGCGAAGAAGTTGGGGAAAT
TGACTACGATGCTGACGCTGAACTAAAGCTAGGTGCTACCTATCCAGAAGGTGAAGATGTAGCAGCAGAATTACTATGTA
TTCAGCAAACGGAAGAAGAAGTAATGGATGGAGAAGAAGGTGCAGAAGTCGAAAAAGCACAGCTAGAAGCTCGTCTTATG
GCGCAGCGTATTAAAGCGATGGTTGATTCAGGTTATGAAGTGTATGATCGTAAAACGGATAGTATGCGCCCTGTACAATA
CCGTGATTTCGTTATTTTACTCCGCTCGATGCCGTGGGCGCCGCAAATTATGGAAGAGTTAAAATTGCAAGGAATTCCAG
TATATGCTGACCTTGCGACTGGTTATTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATTCCGCTTGCAGCTGTACTTCGTTCACCGATTGTGGGACTAAATGATGAAGACCTTGCGACGCTTCG
TGCTCATGGGAAGAAAGGGTCATTTTATGAAGTAATGAGCTCGTTCTTAAAAGGAGCACCGCTTGAAGAAGAGCAAGAAC
TTCATGATAAATTAGAGTGGTTTTATAATTTACTGCAAGGATGGCGTGAATTTGCCCGTCAACAATCCCTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTACGACTTCGTTGGCGGTTTACCAGCTGGAAAGCAAAGGCAGGCAAACTT
GCGCGTGCTATATGACCGTGCAAGACAATATGAAGCAACATCATTTAGAGGGTTATTCCGCTTCTTGCGTTTTATTGAAC
GTATTTTAGAACGCGGTGATGATATGGGTACGGCGAGAGCCCTCGGTGAACAAGAAGACGTTGTTCGCATTATGACGATT
CATAAAAGTAAAGGGCTAGAGTTCCCGGTCGTATTTGTAGCTGGACTCGGTCGTCGTTTTAATACACAAGACTTAATGAA
GCGTTTCTTACTGCATAAAGATTTCGGTTTCGGTTCACAATTTATCGATCCGCGTAAACGAATTAAATATACGACATTAT
CACAGCTTGCGATTAAGCGTAAAATGAAAATGGAATTAATTGCAGAAGAAATGCGCGTATTATACGTAGCGTTAACGCGT
GCGAAAGAGAAGCTAATTTTAATCGGAACAGTTAAGGATGCAACTAAGGAAATGGAAAAATGGCTTGATGCGAGGGAACA
TAGTGAATGGTTATTACCAGATCACATACGTGCTGGGGCGTCTTGTTATTTAGACTGGATTGCACCTTCATTATATAGAC
ACCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAGCATTCCGGATGAAATTTACGGGTATGACACTAGCTGGAAA
GTAGAAGTTGTGGACGGTAACACGCTTCTTGCACCAGAGCCAGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCGCTTCG
TGAGAAAAAGGCTGTTCCCCTGCAAAGTGAACGGAAAGAAGAAGTGTACGACAGATTAATGTGGAAGTACGGATATGAGG
ATGCGACATCTCATCGTGCGAAGCAATCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCC
TTTATTAAAAAACTACGTGCACCAATTAAAACACGTCCGCGCTTTATGGAGAAAAAAGGGTTAACGTACGCAGAGCGTGG
AACAGCAGTCCATGCCGTTATGCAGCATGTAGATTTGAAGAAACCGATTACGGCCGAAACGCTACAAGAACAAATCGCAG
GAATGATAAATAAGGAATTATTAACATTCGAGCAGGCGGAAGAAATAGCAATTGAAAAAGTAATTTCATTCTTTGACAGT
GACTTAGGTAAAAGGGTATTAGCGGCAAAAAGTGTAGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGGAAGAGTGAAGAAACGATTCTTGTCCAAGGGGTTATCGACTGCATGATCGAAGAGGAAGATG
GCATTACGTTAATCGACTTCAAAACAGATACGATTGAAGGGAAATTCCCAGGCGGATTCGAACAAGCAAAACCAATTTTA
GAAGATCGATATAAAGTGCAACTTTCGTTATATGCAAAAGCACTCGAGAAAAGCTTACAACATCCTGTCAAAGAGAAATG
TTTATATTTCTTTGATGGGAATCACGTTGTAAATATTGAAGAATAG

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1231)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.248	100	0.535