Detailed information NTG0493562

Bioinformatically predicted

Overview

• Name: comEC
• Type: Machinery gene
• Locus tag: IMZ24_RS07000
• Genome accession: NZ_CP063157
• Coordinates: 1356818..1359169 (+)
• Length: 783 a.a.
• NCBI ID: WP_032873958.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain GY65
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1351818..1364169

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IMZ24_RS06960 (IMZ24_06960)	yhbY	1352089..1352379 (+)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
IMZ24_RS06965 (IMZ24_06965)	-	1352390..1352959 (+)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
IMZ24_RS06970 (IMZ24_06970)	yqeK	1352949..1353524 (+)	576	WP_032873949.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
IMZ24_RS06975 (IMZ24_06975)	rsfS	1353528..1353884 (+)	357	WP_007408260.1	ribosome silencing factor	-
IMZ24_RS06980 (IMZ24_06980)	-	1353881..1354618 (+)	738	WP_032873951.1	class I SAM-dependent methyltransferase	-
IMZ24_RS06985 (IMZ24_06985)	comER	1354687..1355508 (-)	822	WP_012118027.1	late competence protein ComER	-
IMZ24_RS06990 (IMZ24_06990)	comEA	1355567..1356181 (+)	615	WP_032873955.1	helix-hairpin-helix domain-containing protein	Machinery gene
IMZ24_RS06995 (IMZ24_06995)	-	1356248..1356817 (+)	570	WP_003152868.1	ComE operon protein 2	-
IMZ24_RS07000 (IMZ24_07000)	comEC	1356818..1359169 (+)	2352	WP_032873958.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
IMZ24_RS07005 (IMZ24_07005)	-	1359188..1359322 (-)	135	WP_003152870.1	YqzM family protein	-
IMZ24_RS07010 (IMZ24_07010)	-	1359363..1359514 (+)	152	Protein_1383	hypothetical protein	-
IMZ24_RS07015 (IMZ24_07015)	holA	1359554..1360595 (+)	1042	Protein_1384	DNA polymerase III subunit delta	-
IMZ24_RS07020 (IMZ24_07020)	rpsT	1360612..1360878 (-)	267	WP_003152876.1	30S ribosomal protein S20	-
IMZ24_RS07025 (IMZ24_07025)	gpr	1361081..1362187 (+)	1107	WP_007408268.1	GPR endopeptidase	-
IMZ24_RS07030 (IMZ24_07030)	spoIIP	1362255..1363448 (+)	1194	WP_032873961.1	stage II sporulation protein P	-
IMZ24_RS07035 (IMZ24_07035)	-	1363465..1363803 (+)	339	WP_007408270.1	YqxA family protein	-

Sequence

Protein

• Length: 783 a.a.
• Molecular weight: 86681.60 Da
• Isoelectric Point: 9.1106

>NTDB_id=493562 IMZ24_RS07000 WP_032873958.1 1356818..1359169(+) (comEC) [Bacillus velezensis strain GY65]
MKYKYLLLPLAAVSATAGIAAAHVFWVLLLFLLYLLFIIVKTKHHAPVIVCLVSFCVYFFLYTVCDAANVTRYQAGSYTE
QAVITNIPKVDGAKMSAVIRTHDKEKWAASYKIRSLEEKRLIEQLEPGMRCTFTGSLEQPAHATVPGGFDYKEYLHSQQI
HWLFSVTSIQQCEKSKQPLFKLLNIRKNLISIIRNHVPESSAGIVEALTLGEKFSIEDDILSAYQNLGVVHLMAISGMHV
GLITAGLFYALIRIGLTREKAGILLLLFLPVYTLLSGAAPSVLRASLMLGFYIAGTLVKRGIHSSAALSLSYLLLLMFNP
YFLWQAGFQLSFAVSASLILSSSILKKAGKSRLAGLAMASFIAELSSLPFLLYHFQQISLVSFLMNMVLVPFYTLFVIPV
SVIGFLLLLLSRQMGECLFDMFDLVMKPVHDFITYAASVDLFTMIVSKPDFVSLLLLAVSVFTLFAALEKGGFLKLRKSA
LFFCAVLAYLICRPYFSPWGEADMLDIGQGDSLFISAPHRKGTVMVDTGGVISYPGESWKEKRHPYSIGEKVLIPFLNGK
GVKKLDALILTHADQDHIGEAGVLIKNHRVKRLIVPVGFVKEPKDQNILNMAKENNIPVAEAKRGDTITAGDLQFQVLSP
ESSDGRSKNDSSLVLWTVLGGVSWLLTGDLESDGETEVLKTYPKLKADILKAGHHGSKSSTSEAFLKQLQPEAALISAGK
ENRYHHPHEEVLDRLKVYSVNVLRTDVSGTIQYRFKKGAGTFSVFPPYDIEETRAQEVKKTAD

Nucleotide

• Length: 2352 bp

>NTDB_id=493562 IMZ24_RS07000 WP_032873958.1 1356818..1359169(+) (comEC) [Bacillus velezensis strain GY65]
ATGAAATATAAATACCTTCTTCTGCCTCTGGCGGCGGTTTCTGCAACTGCGGGAATTGCCGCCGCTCATGTCTTCTGGGT
TCTGCTCCTTTTTCTTCTGTATCTTCTCTTTATTATTGTAAAAACAAAGCACCATGCTCCGGTTATCGTCTGCCTCGTTT
CTTTTTGTGTTTATTTCTTTCTTTATACGGTTTGTGACGCTGCGAATGTAACGCGGTATCAGGCCGGCAGTTATACTGAA
CAGGCCGTCATCACTAATATTCCGAAGGTTGACGGAGCGAAAATGTCAGCCGTTATCCGTACACATGACAAGGAAAAATG
GGCGGCTTCGTACAAAATCCGTTCTCTTGAGGAAAAAAGACTTATTGAACAGCTTGAACCGGGGATGCGCTGCACGTTTA
CAGGCTCTCTGGAACAGCCTGCACATGCGACGGTTCCCGGAGGTTTTGATTATAAGGAATATCTTCACTCTCAGCAGATT
CACTGGTTATTTTCCGTGACTTCCATTCAGCAGTGTGAAAAATCCAAACAGCCGCTGTTTAAACTGCTGAACATCAGAAA
AAATTTGATTTCGATCATTCGGAATCACGTGCCTGAATCTTCCGCCGGAATTGTTGAAGCGCTGACCTTAGGTGAAAAAT
TTTCTATAGAGGACGATATACTGAGTGCATATCAAAATTTGGGAGTCGTTCATTTAATGGCGATTTCCGGAATGCATGTC
GGTCTTATTACGGCGGGATTATTTTATGCTCTGATCAGAATCGGGCTGACAAGAGAAAAAGCGGGAATTTTGCTGCTGCT
GTTTTTGCCGGTGTATACGCTGCTGAGCGGTGCCGCCCCATCCGTATTGCGCGCATCCCTCATGCTGGGATTTTATATCG
CCGGAACTCTTGTTAAACGCGGCATTCATTCCTCTGCTGCATTGTCCCTGTCTTATCTGCTGCTCCTGATGTTTAATCCT
TACTTCCTTTGGCAGGCGGGCTTCCAGCTTTCCTTTGCGGTAAGCGCCTCTTTAATTCTGTCATCCTCCATTTTAAAGAA
AGCGGGGAAAAGCAGACTTGCCGGGCTTGCGATGGCTTCATTCATTGCGGAGCTCAGCTCACTTCCGTTTCTTCTCTATC
ATTTTCAGCAGATTTCACTTGTCAGTTTTCTGATGAATATGGTGTTGGTGCCATTTTATACGTTATTTGTCATTCCGGTT
TCTGTTATCGGTTTCCTTCTTCTTTTACTTTCAAGGCAGATGGGAGAATGTTTGTTTGATATGTTCGACCTTGTGATGAA
GCCTGTGCATGATTTCATTACATATGCGGCATCCGTTGATTTATTTACTATGATTGTGTCAAAGCCTGATTTTGTTTCCC
TTCTTCTGCTTGCGGTTTCAGTTTTTACGCTTTTTGCGGCTTTGGAAAAGGGAGGTTTTTTAAAACTCAGGAAATCGGCT
CTTTTTTTCTGCGCGGTTTTGGCTTATTTAATATGCCGTCCGTATTTCAGTCCATGGGGAGAAGCGGATATGCTTGATAT
CGGGCAGGGAGACTCACTGTTTATAAGCGCGCCGCACCGCAAAGGGACCGTAATGGTTGATACAGGGGGAGTGATTTCTT
ATCCCGGAGAATCATGGAAAGAAAAACGCCACCCGTATTCTATCGGCGAGAAGGTTTTGATTCCGTTTTTAAACGGAAAA
GGGGTGAAAAAGCTGGATGCGCTGATTTTAACCCATGCGGATCAAGATCACATCGGAGAAGCCGGAGTGTTAATCAAAAA
TCATAGAGTCAAACGGTTAATTGTCCCCGTGGGATTCGTAAAAGAACCGAAAGATCAGAACATATTAAATATGGCGAAAG
AAAACAACATTCCCGTTGCCGAAGCAAAGCGGGGCGACACCATTACAGCCGGTGATCTTCAGTTTCAGGTGCTGTCTCCG
GAGTCGTCTGACGGAAGGAGTAAAAATGATTCGTCACTGGTGCTTTGGACGGTTTTAGGCGGAGTGAGCTGGCTTTTGAC
GGGAGATTTAGAATCGGACGGCGAAACGGAAGTGCTGAAAACGTATCCGAAACTGAAGGCTGATATATTGAAGGCGGGTC
ATCACGGCAGCAAAAGCTCTACGAGTGAAGCCTTTTTGAAACAGCTTCAGCCGGAAGCAGCGCTGATTTCAGCAGGAAAA
GAGAATCGATACCATCATCCGCATGAAGAAGTGCTGGATCGTTTGAAGGTGTACTCTGTCAATGTGCTTCGCACCGATGT
CAGCGGAACGATTCAATACAGATTTAAAAAAGGCGCCGGAACGTTTTCCGTCTTCCCTCCATATGATATAGAAGAAACCA
GGGCGCAAGAAGTAAAAAAGACTGCCGATTGA

Domains

Predicted by InterproScan.

• Metallo-beta-lactamase superfamily (Lactamase_B) (508-719)

• Competence protein (Competence) (208-469)

• Domain of unknown function (DUF4131) (DUF4131) (25-171)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC	Bacillus subtilis subsp. subtilis str. 168	56.606	98.595	0.558