Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   IMZ18_RS16540 Genome accession   NZ_CP063151
Coordinates   3107954..3108403 (+) Length   149 a.a.
NCBI ID   WP_014475770.1    Uniprot ID   A0A0D1KPR3
Organism   Bacillus subtilis subsp. subtilis strain CMIN-4     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3102954..3113403
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IMZ18_RS16520 (IMZ18_16520) hxlR 3104168..3104530 (-) 363 WP_003246265.1 transcriptional activator HxlR -
  IMZ18_RS16525 (IMZ18_16525) hxlA 3104759..3105391 (+) 633 WP_014478804.1 3-hexulose-6-phosphate synthase -
  IMZ18_RS16530 (IMZ18_16530) hxlB 3105397..3105954 (+) 558 WP_014478803.1 6-phospho-3-hexuloisomerase -
  IMZ18_RS16535 (IMZ18_16535) tlpC 3106065..3107786 (+) 1722 Protein_3273 methyl-accepting chemotaxis protein TlpC -
  IMZ18_RS16540 (IMZ18_16540) nucA/comI 3107954..3108403 (+) 450 WP_014475770.1 DNA-entry nuclease Machinery gene
  IMZ18_RS16545 (IMZ18_16545) nin/comJ 3108430..3108828 (+) 399 WP_014478800.1 DNA-entry nuclease inhibitor Regulator
  IMZ18_RS16550 (IMZ18_16550) bglC 3108865..3110298 (-) 1434 WP_014478799.1 6-phospho-beta-glucosidase -
  IMZ18_RS16555 (IMZ18_16555) - 3110371..3111115 (-) 745 Protein_3277 AAA family ATPase -
  IMZ18_RS16560 (IMZ18_16560) - 3111134..3111454 (-) 321 WP_014478796.1 DUF3147 family protein -
  IMZ18_RS16565 (IMZ18_16565) - 3111442..3111864 (-) 423 WP_014478795.1 MarR family transcriptional regulator -
  IMZ18_RS16570 (IMZ18_16570) yckD 3111992..3112324 (-) 333 WP_014478794.1 YckD family protein -
  IMZ18_RS16575 (IMZ18_16575) yckC 3112406..3112861 (-) 456 WP_014478793.1 RDD family protein -
  IMZ18_RS16580 (IMZ18_16580) - 3112881..3113078 (-) 198 WP_121509395.1 hypothetical protein -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16440.39 Da        Isoelectric Point: 4.5282

>NTDB_id=493494 IMZ18_RS16540 WP_014475770.1 3107954..3108403(+) (nucA/comI) [Bacillus subtilis subsp. subtilis strain CMIN-4]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=493494 IMZ18_RS16540 WP_014475770.1 3107954..3108403(+) (nucA/comI) [Bacillus subtilis subsp. subtilis strain CMIN-4]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAAGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1KPR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

98.658

100

0.987