Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   IMZ18_RS05475 Genome accession   NZ_CP063151
Coordinates   1028113..1028880 (+) Length   255 a.a.
NCBI ID   WP_014480292.1    Uniprot ID   -
Organism   Bacillus subtilis subsp. subtilis strain CMIN-4     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 1023113..1033880
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IMZ18_RS05445 (IMZ18_05445) pgpH 1023409..1025544 (+) 2136 WP_014480296.1 cyclic-di-AMP phosphodiesterase PgpH -
  IMZ18_RS05450 (IMZ18_05450) ybeY 1025545..1026018 (+) 474 WP_014480295.1 rRNA maturation RNase YbeY -
  IMZ18_RS05455 (IMZ18_05455) unpK 1025999..1026370 (+) 372 WP_014480294.1 diacylglycerol kinase family protein -
  IMZ18_RS05460 (IMZ18_05460) - 1026489..1026899 (+) 411 WP_014480293.1 cytidine deaminase -
  IMZ18_RS05465 (IMZ18_05465) era 1026880..1027785 (+) 906 WP_003230051.1 GTPase Era -
  IMZ18_RS05470 (IMZ18_05470) yqzL 1027933..1028076 (+) 144 WP_003230053.1 YqzL family protein -
  IMZ18_RS05475 (IMZ18_05475) recO 1028113..1028880 (+) 768 WP_014480292.1 DNA repair protein RecO Machinery gene
  IMZ18_RS05480 (IMZ18_05480) glyQ 1029177..1030064 (+) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  IMZ18_RS05485 (IMZ18_05485) glyS 1030057..1032096 (+) 2040 WP_014480291.1 glycine--tRNA ligase subunit beta -
  IMZ18_RS05490 (IMZ18_05490) ccpN 1032229..1032867 (+) 639 WP_003237058.1 transcriptional regulator CcpN -
  IMZ18_RS05495 (IMZ18_05495) ppsR 1032892..1033704 (+) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29342.05 Da        Isoelectric Point: 8.3080

>NTDB_id=493455 IMZ18_RS05475 WP_014480292.1 1028113..1028880(+) (recO) [Bacillus subtilis subsp. subtilis strain CMIN-4]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDRGTEEKKPNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPLKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=493455 IMZ18_RS05475 WP_014480292.1 1028113..1028880(+) (recO) [Bacillus subtilis subsp. subtilis strain CMIN-4]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACTTTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATATTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGCC
AAATCCTTATTTATTTGAATTCATATTAGAATCATTAAAACAGCTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTGAATCATTGTGTACATTGTAAGAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGCTCAA
ACCGCAAACGGCAAGGCTGTTAAGACTCTTTTACTATTTTGATCTGTCGAGGCTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

99.216

100

0.992