Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   IMY14_RS01950 Genome accession   NZ_CP063150
Coordinates   362639..363088 (-) Length   149 a.a.
NCBI ID   WP_014475770.1    Uniprot ID   A0A0D1KPR3
Organism   Bacillus subtilis strain s-16     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 357639..368088
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IMY14_RS01910 - 357975..358172 (+) 198 WP_144052033.1 hypothetical protein -
  IMY14_RS01915 yckC 358223..358648 (+) 426 WP_047182064.1 RDD family protein -
  IMY14_RS01920 yckD 358730..359062 (+) 333 WP_047182065.1 YckD family protein -
  IMY14_RS01925 - 359174..359596 (+) 423 WP_174616579.1 MarR family winged helix-turn-helix transcriptional regulator -
  IMY14_RS01930 - 359584..359925 (+) 342 WP_032724911.1 DUF3147 family protein -
  IMY14_RS01935 - 359944..360672 (+) 729 WP_021481826.1 AAA family ATPase -
  IMY14_RS01940 bglC 360744..362177 (+) 1434 WP_047182066.1 6-phospho-beta-glucosidase -
  IMY14_RS01945 nin/comJ 362214..362612 (-) 399 WP_015252845.1 DNA-entry nuclease inhibitor Regulator
  IMY14_RS01950 nucA/comI 362639..363088 (-) 450 WP_014475770.1 DNA-entry nuclease Machinery gene
  IMY14_RS01955 tlpC 363256..364977 (-) 1722 WP_047182067.1 methyl-accepting chemotaxis protein TlpC -
  IMY14_RS01960 hxlB 365088..365645 (-) 558 WP_047182068.1 6-phospho-3-hexuloisomerase -
  IMY14_RS01965 hxlA 365651..366283 (-) 633 WP_047182069.1 3-hexulose-6-phosphate synthase -
  IMY14_RS01970 hxlR 366515..366877 (+) 363 WP_047182070.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16440.39 Da        Isoelectric Point: 4.5282

>NTDB_id=493365 IMY14_RS01950 WP_014475770.1 362639..363088(-) (nucA/comI) [Bacillus subtilis strain s-16]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=493365 IMY14_RS01950 WP_014475770.1 362639..363088(-) (nucA/comI) [Bacillus subtilis strain s-16]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCTGAGGTGTGCACTATTGACAGAGACGGAGCT
GAAGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1KPR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

98.658

100

0.987