Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   KIM36_RS04910 Genome accession   NZ_CP074811
Coordinates   997261..997701 (-) Length   146 a.a.
NCBI ID   WP_000360902.1    Uniprot ID   A7ZHJ7
Organism   Escherichia coli strain 7_56_38A     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 992261..1002701
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIM36_RS04885 (KIM36_04810) coaE 992751..993371 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  KIM36_RS04890 (KIM36_04815) - 993396..993440 (+) 45 WP_120795372.1 protein YacM -
  KIM36_RS04895 (KIM36_04820) guaC 993596..994639 (+) 1044 WP_001217338.1 GMP reductase -
  KIM36_RS04900 (KIM36_04825) hofC 994674..995876 (-) 1203 WP_000157234.1 protein transport protein HofC -
  KIM36_RS04905 (KIM36_04830) pilB 995866..997251 (-) 1386 WP_001025155.1 type II secretion system protein GspE Machinery gene
  KIM36_RS04910 (KIM36_04835) pilA 997261..997701 (-) 441 WP_000360902.1 prepilin peptidase-dependent pilin Machinery gene
  KIM36_RS04915 (KIM36_04840) nadC 997904..998797 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  KIM36_RS04920 (KIM36_04845) ampD 998885..999436 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  KIM36_RS04925 (KIM36_04850) ampE 999433..1000287 (+) 855 WP_000172006.1 beta-lactamase regulator AmpE -
  KIM36_RS04930 (KIM36_04855) aroP 1000330..1001703 (-) 1374 WP_012817732.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15630.76 Da        Isoelectric Point: 4.5559

>NTDB_id=492962 KIM36_RS04910 WP_000360902.1 997261..997701(-) (pilA) [Escherichia coli strain 7_56_38A]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=492962 KIM36_RS04910 WP_000360902.1 997261..997701(-) (pilA) [Escherichia coli strain 7_56_38A]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCATC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZHJ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

41.791

91.781

0.384
  pilA Haemophilus influenzae Rd KW20

41.045

91.781

0.377