Detailed information NTG0492657

Bioinformatically predicted

Overview

• Name: radA
• Type: Machinery gene
• Locus tag: IMX20_RS00355
• Genome accession: NZ_CP063065
• Coordinates: 79257..80639 (+)
• Length: 460 a.a.
• NCBI ID: WP_026465600.1
• Uniprot ID: A0A7M1KTN5
• Organism: Aerococcus urinaeequi strain T43
• Function: homologous recombination (predicted from homology) Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Prophage	78670..99729	79257..80639	within	0

Gene organization within MGE regions

Location: 78670..99729

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IMX20_RS00350 (IMX20_00350)	-	78670..79209 (+)	540	WP_197558539.1	dUTP diphosphatase	-
IMX20_RS00355 (IMX20_00355)	radA	79257..80639 (+)	1383	WP_026465600.1	DNA repair protein RadA	Machinery gene
IMX20_RS00360 (IMX20_00360)	-	80856..82049 (+)	1194	WP_197558540.1	PIN/TRAM domain-containing protein	-
IMX20_RS00365 (IMX20_00365)	gltX	82136..83611 (+)	1476	WP_083068298.1	glutamate--tRNA ligase	-
IMX20_RS00370 (IMX20_00370)	-	83963..84202 (+)	240	WP_016896486.1	glutaredoxin domain-containing protein	-
IMX20_RS00375 (IMX20_00375)	nrdI	84190..84594 (+)	405	WP_016896485.1	class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI	-
IMX20_RS00380 (IMX20_00380)	nrdE	84566..86764 (+)	2199	WP_197558541.1	class 1b ribonucleoside-diphosphate reductase subunit alpha	-
IMX20_RS00385 (IMX20_00385)	nrdF	86790..87806 (+)	1017	WP_197558542.1	class 1b ribonucleoside-diphosphate reductase subunit beta	-
IMX20_RS00390 (IMX20_00390)	-	87990..88586 (+)	597	WP_026465606.1	xanthine phosphoribosyltransferase	-
IMX20_RS00395 (IMX20_00395)	-	88590..89882 (+)	1293	WP_197558543.1	nucleobase:cation symporter-2 family protein	-
IMX20_RS00400 (IMX20_00400)	-	90110..91066 (+)	957	WP_026465608.1	2-keto-3-deoxygluconate permease	-
IMX20_RS00405 (IMX20_00405)	-	91325..92863 (+)	1539	WP_197558544.1	ABC transporter permease/substrate-binding protein	-
IMX20_RS00410 (IMX20_00410)	-	92863..93801 (+)	939	WP_197558545.1	ABC transporter ATP-binding protein	-
IMX20_RS00415 (IMX20_00415)	-	93841..94389 (+)	549	WP_197558546.1	GNAT family N-acetyltransferase	-
IMX20_RS00420 (IMX20_00420)	-	94711..96123 (+)	1413	WP_332104956.1	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase	-
IMX20_RS00425 (IMX20_00425)	-	96177..96860 (+)	684	Protein_83	IS6-like element IS1216 family transposase	-
IMX20_RS00430 (IMX20_00430)	-	97013..98272 (-)	1260	WP_197558547.1	cytochrome P450	-
IMX20_RS00435 (IMX20_00435)	-	98459..99043 (+)	585	WP_197558548.1	hypothetical protein	-
IMX20_RS00440 (IMX20_00440)	-	99116..99343 (+)	228	WP_148129216.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
IMX20_RS00445 (IMX20_00445)	-	99340..99729 (+)	390	WP_082148263.1	type II toxin-antitoxin system death-on-curing family toxin	-

Sequence

Protein

• Length: 460 a.a.
• Molecular weight: 50260.45 Da
• Isoelectric Point: 6.6987

>NTDB_id=492657 IMX20_RS00355 WP_026465600.1 79257..80639(+) (radA) [Aerococcus urinaeequi strain T43]
MAKKTTTRYVCQACGYESVQFYGKCPNCGAWNQMEEERLYGKTIAHPETRVGQANSSRMKPQKLAAVKGEETPRIQTQLG
ELNRVLGGGVVQGSLVLIGGDPGIGKSTLMLQVSAELHQSAGPVLYVSGEESMHQIKLRADRLGFEGADFYVYAETDMAA
IQDAIYDLQPRFVVIDSIQTMTHEDATSTAGSVGQVRQTTAELMKLAKSQNIGIFIVGHVTKEGNIAGPRILEHMVDTVL
YFEGEKHDTFRILRAVKNRFGSTNEIGVFDMQQDGLHEVANPSELFLEERLAGTNGSAVVASMEGTRPILTEIQALLTPT
SFGNARRTASGLDYSRVSLIMAVLEKRAGLMLQNQDAYLKSTGGVRLDEPAIDLAIAVAVASSYREKETKPTDCFIGEIG
LTGEIRRVTRIAERVQEAEKLGFERIFIPKNAISGLTGKQKIQVVGVTTVREVLNTIFPK

Nucleotide

• Length: 1383 bp

>NTDB_id=492657 IMX20_RS00355 WP_026465600.1 79257..80639(+) (radA) [Aerococcus urinaeequi strain T43]
ATGGCGAAGAAAACGACAACGAGATATGTGTGTCAGGCTTGTGGTTATGAGAGTGTTCAGTTCTATGGCAAGTGTCCGAA
TTGTGGGGCTTGGAACCAAATGGAAGAGGAACGCTTATATGGGAAGACTATTGCTCATCCTGAAACCCGCGTTGGACAAG
CCAATTCAAGCCGGATGAAGCCACAAAAATTAGCGGCGGTTAAAGGGGAAGAAACCCCGCGTATCCAAACCCAATTAGGC
GAATTAAATCGCGTCTTAGGTGGCGGTGTGGTGCAAGGTTCGCTGGTTTTAATTGGTGGTGACCCAGGGATTGGGAAGTC
AACCTTAATGCTACAGGTATCAGCTGAATTGCATCAGAGTGCTGGGCCAGTTTTATATGTGTCCGGAGAGGAATCGATGC
ATCAAATCAAGCTACGAGCAGACCGACTAGGATTTGAAGGTGCAGATTTCTATGTTTATGCAGAAACTGATATGGCGGCT
ATCCAAGATGCCATTTATGACTTACAACCGCGCTTTGTAGTGATTGACTCTATTCAAACTATGACCCACGAGGATGCGAC
ATCAACGGCTGGATCAGTTGGCCAAGTCCGCCAAACGACAGCAGAGTTAATGAAATTAGCGAAGTCACAAAATATCGGTA
TTTTTATCGTTGGCCATGTGACGAAAGAAGGAAACATTGCTGGACCGCGTATTCTAGAACACATGGTGGATACTGTACTG
TATTTTGAAGGTGAGAAGCATGATACTTTCAGGATTTTACGGGCAGTGAAGAACCGGTTTGGGTCAACCAATGAAATAGG
TGTCTTTGACATGCAACAAGACGGCCTGCATGAAGTCGCTAACCCTTCCGAACTCTTCTTAGAAGAAAGACTAGCTGGCA
CTAATGGATCAGCAGTTGTTGCTTCGATGGAAGGAACACGACCGATTTTAACGGAAATTCAAGCACTACTTACACCTACC
TCATTTGGGAATGCCCGTAGAACAGCTTCTGGCTTAGATTATTCACGGGTATCACTAATTATGGCTGTACTTGAGAAGAG
AGCCGGTTTAATGCTTCAAAATCAAGATGCCTACCTCAAATCAACTGGTGGCGTCCGTTTAGATGAACCAGCGATTGACC
TGGCTATCGCTGTAGCTGTGGCTTCTTCATACCGAGAAAAAGAAACCAAACCAACTGATTGTTTCATTGGAGAAATTGGT
TTGACTGGTGAAATTAGACGGGTAACAAGAATTGCGGAACGTGTTCAAGAAGCTGAAAAACTAGGTTTTGAGCGTATTTT
TATCCCAAAAAATGCGATATCAGGTTTAACTGGCAAACAAAAAATACAAGTAGTTGGTGTAACTACTGTAAGAGAAGTGT
TAAATACGATTTTTCCAAAATAG

Domains

Predicted by InterproScan.

• Subunit ChlI of Mg-chelatase (ChlI) (370-431)

• Rubredoxin metal binding domain (Zn_ribbon_LapB) (9-36)

• AAA domain (AAA_25) (72-224)

3D structure

• AlphaFold DB: A0A7M1KTN5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
radA	Streptococcus pneumoniae Rx1	63.974	99.565	0.637
radA	Streptococcus mitis NCTC 12261	63.974	99.565	0.637
radA	Streptococcus mitis SK321	63.974	99.565	0.637
radA	Streptococcus pneumoniae TIGR4	63.974	99.565	0.637
radA	Streptococcus pneumoniae D39	63.974	99.565	0.637
radA	Streptococcus pneumoniae R6	63.974	99.565	0.637
radA	Bacillus subtilis subsp. subtilis str. 168	63.797	98.478	0.628