Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KIM40_RS02265 Genome accession   NZ_CP074785
Coordinates   488130..488597 (-) Length   155 a.a.
NCBI ID   WP_000339836.1    Uniprot ID   -
Organism   Escherichia coli strain 95_0274     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 483130..493597
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIM40_RS02240 (KIM40_02235) rpsG 483271..483741 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  KIM40_RS02245 (KIM40_02240) fusA 483838..485952 (+) 2115 WP_000124700.1 elongation factor G -
  KIM40_RS02250 (KIM40_02245) tuf 486023..487207 (+) 1185 WP_000031783.1 elongation factor Tu -
  KIM40_RS02255 (KIM40_02250) bfd 487390..487584 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  KIM40_RS02260 (KIM40_02255) bfr 487657..488133 (+) 477 WP_000675515.1 bacterioferritin -
  KIM40_RS02265 (KIM40_02260) pilD 488130..488597 (-) 468 WP_000339836.1 prepilin peptidase Machinery gene
  KIM40_RS02270 (KIM40_02265) rpsJ 488976..489287 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  KIM40_RS02275 (KIM40_02270) rplC 489320..489949 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  KIM40_RS02280 (KIM40_02275) rplD 489960..490565 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  KIM40_RS02285 (KIM40_02280) rplW 490562..490864 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  KIM40_RS02290 (KIM40_02285) rplB 490882..491703 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  KIM40_RS02295 (KIM40_02290) rpsS 491720..491998 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  KIM40_RS02300 (KIM40_02295) rplV 492013..492345 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  KIM40_RS02305 (KIM40_02300) rpsC 492363..493064 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  KIM40_RS02310 (KIM40_02305) rplP 493077..493487 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16951.08 Da        Isoelectric Point: 8.1296

>NTDB_id=492633 KIM40_RS02265 WP_000339836.1 488130..488597(-) (pilD) [Escherichia coli strain 95_0274]
MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=492633 KIM40_RS02265 WP_000339836.1 488130..488597(-) (pilD) [Escherichia coli strain 95_0274]
ATGGACGCTAACCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTAGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361