Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   INP81_RS00585 Genome accession   NZ_CP063057
Coordinates   132757..134142 (-) Length   461 a.a.
NCBI ID   WP_012836620.1    Uniprot ID   A0A5S4SXP0
Organism   Comamonas thiooxydans strain ZDHYF418     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 127757..139142
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INP81_RS00555 (INP81_00555) - 127788..128552 (+) 765 WP_034351307.1 glycosyltransferase family 25 protein -
  INP81_RS00560 (INP81_00560) - 128542..129675 (-) 1134 WP_054073387.1 glycosyltransferase -
  INP81_RS00565 (INP81_00565) - 129738..130703 (+) 966 WP_174447077.1 glycosyltransferase family 32 protein -
  INP81_RS00570 (INP81_00570) - 130749..130958 (-) 210 WP_003059801.1 zinc-finger domain-containing protein -
  INP81_RS00575 (INP81_00575) - 131101..132039 (-) 939 WP_034382721.1 branched-chain amino acid transaminase -
  INP81_RS00580 (INP81_00580) - 132121..132576 (-) 456 WP_034351309.1 hypothetical protein -
  INP81_RS00585 (INP81_00585) radA 132757..134142 (-) 1386 WP_012836620.1 DNA repair protein RadA Machinery gene
  INP81_RS00595 (INP81_00595) - 134564..135871 (-) 1308 WP_034351570.1 Glu/Leu/Phe/Val family dehydrogenase -
  INP81_RS00600 (INP81_00600) - 136081..137004 (+) 924 WP_054073386.1 fumarylacetoacetate hydrolase family protein -
  INP81_RS00605 (INP81_00605) - 137159..138268 (-) 1110 WP_174447065.1 PPK2 family polyphosphate kinase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 48073.39 Da        Isoelectric Point: 7.6670

>NTDB_id=492618 INP81_RS00585 WP_012836620.1 132757..134142(-) (radA) [Comamonas thiooxydans strain ZDHYF418]
MAKEKTTFTCNACGGTSPRWLGKCPGCGAWNTLVETVADSASGGKNRLSQPQGYAGLANAQPVTPLSAIEAQDVARTPSG
IEELDRVLGGGVVEGGVVLIGGDPGIGKSTLLLQAMDALHRIGLPTLYVTGEESGAQVALRSRRLGLDNSQVNVLAEIQL
EKILATVEATQPAVVVIDSIQTVYSDQLSSAPGSVAQVRECAAHLTRAAKTTGITIILVGHVTKDGALAGPRVLEHMVDT
VLYFEGDTHSNFRLVRAIKNRFGAVNEIGVFAMTEKGLKGVTNPSAIFLSQHSEPVPGSCVLVTLEGTRPMLVEIQALVD
QGGPAARRLSVGLDRDRLAMLLAVLNRHAGVACADQDVFVNAVGGVRINEPAADLAVMLAITSSLRGKSLPKGFIAFGEV
GLAGEVRPAPRGQERLKEAAKLGFTIAVVPKANAPKKPIAGLTIHAVERVDEAINIVRGIG

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=492618 INP81_RS00585 WP_012836620.1 132757..134142(-) (radA) [Comamonas thiooxydans strain ZDHYF418]
ATGGCCAAAGAGAAAACCACTTTCACCTGCAACGCCTGTGGCGGCACCAGCCCGCGCTGGCTGGGCAAATGCCCGGGCTG
CGGCGCTTGGAACACCCTGGTCGAGACCGTGGCCGACTCCGCCTCGGGCGGCAAGAATCGCCTGAGCCAGCCCCAGGGCT
ATGCAGGTCTGGCCAATGCCCAGCCCGTCACTCCGCTGTCGGCCATCGAGGCCCAGGATGTGGCGCGCACCCCCAGCGGT
ATCGAGGAGCTGGACCGCGTGCTGGGCGGCGGCGTGGTCGAAGGCGGCGTGGTGCTGATTGGCGGAGATCCTGGAATAGG
CAAGTCCACACTTCTTCTGCAGGCCATGGATGCATTGCATCGTATCGGTCTGCCCACGCTCTATGTGACGGGCGAGGAAA
GCGGCGCCCAGGTGGCGCTGCGCTCGCGCCGGCTGGGGCTGGACAACAGCCAGGTCAATGTGCTGGCCGAGATCCAGCTC
GAGAAAATCCTGGCCACCGTGGAGGCCACCCAGCCCGCCGTGGTGGTCATCGACTCGATTCAGACCGTGTATTCGGACCA
GCTTTCCTCGGCACCGGGCTCCGTGGCCCAGGTGCGCGAGTGCGCGGCCCATCTGACGCGCGCGGCCAAGACCACGGGCA
TCACCATCATTTTGGTGGGCCATGTGACCAAGGACGGCGCGCTGGCCGGCCCACGCGTGCTCGAGCACATGGTGGACACG
GTGCTCTATTTCGAAGGCGACACGCACAGCAACTTCCGTCTGGTGCGCGCCATCAAGAACCGCTTTGGCGCCGTCAACGA
GATCGGCGTCTTCGCGATGACGGAAAAAGGCCTCAAGGGCGTGACCAACCCCAGCGCCATCTTTCTGAGCCAGCACAGCG
AGCCCGTGCCCGGCAGCTGCGTGCTGGTGACACTGGAGGGCACGCGCCCCATGCTGGTGGAAATCCAGGCCCTGGTGGAC
CAGGGCGGCCCGGCCGCGCGCCGCCTCTCCGTCGGCCTGGACCGCGACCGGCTGGCCATGCTGCTGGCCGTGCTCAACCG
CCATGCGGGCGTGGCCTGCGCCGACCAGGATGTCTTCGTCAATGCAGTGGGCGGCGTGCGCATCAATGAGCCTGCAGCAG
ACCTGGCGGTGATGTTAGCAATCACTTCCAGCCTGCGTGGCAAGTCACTGCCCAAGGGCTTTATCGCGTTTGGCGAGGTC
GGTCTGGCCGGCGAGGTGCGCCCCGCCCCTCGGGGTCAGGAGCGACTCAAGGAGGCGGCAAAGTTGGGATTCACTATCGC
CGTGGTTCCCAAGGCCAATGCCCCCAAGAAGCCCATCGCCGGCCTGACCATCCATGCCGTGGAGCGCGTTGATGAAGCCA
TCAACATCGTGCGCGGCATTGGCTGA

Domains

Predicted by InterproScan.

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(364-455)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(68-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5S4SXP0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.02

99.566

0.488
  radA Streptococcus mitis NCTC 12261

47.598

99.349

0.473
  radA Streptococcus pneumoniae D39

48.438

97.18

0.471
  radA Streptococcus pneumoniae TIGR4

48.438

97.18

0.471
  radA Streptococcus pneumoniae R6

48.438

97.18

0.471
  radA Streptococcus pneumoniae Rx1

48.438

97.18

0.471
  radA Streptococcus mitis SK321

49.425

94.36

0.466