Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   IL989_RS00585 Genome accession   NZ_CP062984
Coordinates   106704..108083 (+) Length   459 a.a.
NCBI ID   WP_045514053.1    Uniprot ID   A0AAP7N3R0
Organism   Bacillus amyloliquefaciens strain PP19 isolate PP19     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 101704..113083
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IL989_RS00565 (IL989_00565) ctsR 102052..102516 (+) 465 WP_003156396.1 transcriptional regulator CtsR -
  IL989_RS00570 (IL989_00570) - 102530..103087 (+) 558 WP_013350789.1 UvrB/UvrC motif-containing protein -
  IL989_RS00575 (IL989_00575) - 103087..104178 (+) 1092 WP_013350790.1 protein arginine kinase -
  IL989_RS00580 (IL989_00580) clpC 104175..106610 (+) 2436 WP_013350791.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  IL989_RS00585 (IL989_00585) radA 106704..108083 (+) 1380 WP_045514053.1 DNA repair protein RadA Machinery gene
  IL989_RS00590 (IL989_00590) disA 108087..109169 (+) 1083 WP_193351201.1 DNA integrity scanning diadenylate cyclase DisA -
  IL989_RS00595 (IL989_00595) - 109283..110383 (+) 1101 WP_193351202.1 PIN/TRAM domain-containing protein -
  IL989_RS00600 (IL989_00600) ispD 110397..111095 (+) 699 WP_193351888.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  IL989_RS00605 (IL989_00605) ispF 111088..111564 (+) 477 WP_013350796.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49506.91 Da        Isoelectric Point: 7.1316

>NTDB_id=492217 IL989_RS00585 WP_045514053.1 106704..108083(+) (radA) [Bacillus amyloliquefaciens strain PP19 isolate PP19]
MAKSKTKFICHSCGYESAKWMGKCPGCGAWNTMVEETIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVQTKLEEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLADTAGSVLYISGEESVKQTKLRADRLGINSQLLHVLSETDMEYIS
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGASGSSIVASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMVIPEANADGWTIPKGIEVVGVANVAEALRTSLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=492217 IL989_RS00585 WP_045514053.1 106704..108083(+) (radA) [Bacillus amyloliquefaciens strain PP19 isolate PP19]
ATGGCTAAATCAAAAACAAAATTCATCTGCCACTCATGCGGTTATGAATCCGCAAAATGGATGGGAAAGTGCCCGGGCTG
CGGGGCTTGGAATACAATGGTGGAAGAAACCATAAAAAAAGCGCCGGCGAATCGGCGGGCGGCTTTCTCCCACTCCGTTC
AAACCGTCCAGAAACCATCACCCATTACATCAATTGAAACATCAGAAGAACCCCGCGTTCAAACCAAGCTCGAAGAATTC
AACAGAGTGCTTGGCGGCGGAGTTGTGAAGGGCTCGTTAGTATTAATCGGCGGCGATCCCGGCATCGGAAAATCAACGCT
CCTTCTTCAAGTATCCGCACAATTAGCAGACACGGCCGGCAGCGTCCTTTACATTTCCGGGGAAGAATCTGTGAAGCAAA
CGAAGCTGCGCGCCGACAGACTTGGAATCAACAGCCAATTGTTACATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAAACCCGCATTTGTTGTCGTAGACTCCATTCAGACCGTTTACCAAAGTGATATCACCTCAGC
TCCCGGCAGTGTATCACAAGTAAGAGAGTGTACGGCCGAATTGATGAAAATCGCGAAAACAAACGGCATACCTATTTTTA
TCGTCGGACACGTCACAAAGGAAGGATCGATCGCAGGGCCGAGATTACTTGAGCATATGGTCGATACCGTTCTATACTTT
GAAGGAGAGCGGCACCATACATTCCGTATTTTACGGGCTGTAAAAAACCGTTTCGGCTCAACAAATGAAATGGGCATCTT
TGAAATGCGGGAAGAAGGGCTTACAGAAGTGCTGAACCCTTCTGAAATTTTCTTAGAAGAGCGTTCTGCCGGGGCTTCGG
GTTCGAGTATCGTTGCGTCAATGGAAGGTACGAGACCGATCTTAGTTGAAATCCAGGCGCTCATATCACCGACCAGCTTC
GGAAATCCGCGCCGCATGGCTACAGGAATTGACCACAACAGGGTGTCGTTAATCATGGCTGTGCTTGAGAAGCGGGTGGG
GCTGTTGCTTCAAAATCAAGATGCGTATTTAAAAGTCGCCGGCGGAGTCAAACTGGATGAACCGGCAATTGATTTGGCAG
TAGCCGTCAGCATTGCTTCAAGCTTCAGAGATACACCGCCAAATCCTGCAGATTGCTTTATCGGTGAAGTGGGTCTGACA
GGAGAAGTCCGCAGGGTGTCAAGAATCGAACAGCGCGTAAAAGAAGCGGCGAAGCTCGGCTTCAAGCGTATGGTCATACC
TGAGGCAAATGCAGACGGATGGACAATACCAAAAGGGATTGAGGTTGTCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATCATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(71-222)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

93.231

99.782

0.93
  radA Streptococcus pneumoniae Rx1

63.797

98.693

0.63
  radA Streptococcus pneumoniae D39

63.797

98.693

0.63
  radA Streptococcus pneumoniae R6

63.797

98.693

0.63
  radA Streptococcus pneumoniae TIGR4

63.797

98.693

0.63
  radA Streptococcus mitis NCTC 12261

63.797

98.693

0.63
  radA Streptococcus mitis SK321

63.576

98.693

0.627