Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LPB04_RS03535 Genome accession   NZ_CP062941
Coordinates   802560..804026 (-) Length   488 a.a.
NCBI ID   WP_193687409.1    Uniprot ID   -
Organism   Massilia litorea strain LPB0304     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 797560..809026
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPB04_RS03510 (LPB04_03510) - 798205..798687 (+) 483 WP_056345172.1 PTS sugar transporter subunit IIA -
  LPB04_RS03515 (LPB04_03515) hprK 798738..799673 (+) 936 WP_056345175.1 HPr(Ser) kinase/phosphatase -
  LPB04_RS03520 (LPB04_03520) rapZ 799918..800781 (+) 864 WP_193687406.1 RNase adapter RapZ -
  LPB04_RS03525 (LPB04_03525) - 800941..802026 (-) 1086 WP_193687407.1 enoyl-CoA hydratase/isomerase family protein -
  LPB04_RS03530 (LPB04_03530) hpf 802140..802496 (-) 357 WP_193687408.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  LPB04_RS03535 (LPB04_03535) rpoN/rpoN1 802560..804026 (-) 1467 WP_193687409.1 RNA polymerase factor sigma-54 Machinery gene
  LPB04_RS03540 (LPB04_03540) lptB 804065..804826 (-) 762 WP_193687410.1 LPS export ABC transporter ATP-binding protein -
  LPB04_RS03545 (LPB04_03545) lptA 804826..805416 (-) 591 WP_193687411.1 lipopolysaccharide transport periplasmic protein LptA -
  LPB04_RS03550 (LPB04_03550) lptC 805432..806034 (-) 603 WP_227496604.1 LPS export ABC transporter periplasmic protein LptC -
  LPB04_RS03555 (LPB04_03555) - 806079..806609 (-) 531 WP_193687413.1 HAD family hydrolase -
  LPB04_RS03560 (LPB04_03560) - 806610..807632 (-) 1023 WP_330635406.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 488 a.a.        Molecular weight: 53480.74 Da        Isoelectric Point: 4.9761

>NTDB_id=492037 LPB04_RS03535 WP_193687409.1 802560..804026(-) (rpoN/rpoN1) [Massilia litorea strain LPB0304]
MKQSLQLRTSQHLALTPQLQQSIRLLQLSTLELHQEIEQILSDNPLLERLDDPLDHSVRLLADGAINSAPAQAEAPAPNN
EEGAPAPEAAEGGEGGEVERGESEGDWGEGSRASSGDGDDDGRPQLGAVGVTLREHLCEQIRVTGVTARDRALAELVIDA
IDDNGYLEEQLDEIHARLPEELEVEMDELRCALALVQSLDPVGVGARSASECLALQIRAMPGVPLVTRRMALKIVEGYLS
WFAQREFTKLKKALDCDDEDLREAQAVIRQCNPHPGAAFASSVSDYVVPDVIVRKSKGGWSVSLNNEVMPRLRVNNVYAN
LLKQGKGEGQMSAQLQEAKWLIKNMRQRFDTILRVSQAIVERQKNFFSHGAVAMRPLVLREIADTLGLHESTISRVTTQK
FMLTPHGMFELKYFFGSHVATEAGGEASSTAIRALIAQLTGAEDPKNPLSDSKIAEMLGEQGMVIARRTVAKYREALKIP
PVSLRKSL

Nucleotide


Download         Length: 1467 bp        

>NTDB_id=492037 LPB04_RS03535 WP_193687409.1 802560..804026(-) (rpoN/rpoN1) [Massilia litorea strain LPB0304]
ATGAAACAATCCCTGCAACTCCGGACTTCGCAGCACCTGGCGCTCACGCCGCAGCTTCAGCAGTCGATCCGCCTGCTGCA
GCTCTCCACCCTTGAACTACATCAGGAGATCGAGCAGATCCTGTCCGATAACCCGCTGCTCGAACGGCTCGACGACCCGC
TCGACCACTCGGTGCGCCTGCTGGCCGACGGCGCGATCAACAGCGCGCCGGCGCAAGCCGAGGCGCCGGCGCCGAACAAC
GAGGAGGGCGCCCCTGCGCCCGAGGCGGCCGAGGGCGGCGAAGGCGGCGAGGTCGAACGCGGCGAGTCCGAGGGCGACTG
GGGAGAGGGTTCGCGCGCATCGAGCGGCGACGGCGACGACGACGGCCGCCCGCAGCTCGGCGCGGTCGGCGTGACCCTGC
GCGAGCATCTGTGCGAGCAGATTCGCGTGACGGGCGTCACGGCGCGCGACCGCGCGCTGGCCGAACTCGTGATCGACGCG
ATCGACGACAACGGCTACCTGGAGGAGCAGCTCGACGAGATCCATGCGCGCTTGCCGGAGGAGCTCGAGGTCGAGATGGA
CGAACTGCGCTGCGCGCTGGCGCTGGTGCAAAGCCTGGACCCGGTCGGCGTCGGCGCGCGCAGCGCATCCGAATGCCTGG
CGCTGCAGATCCGCGCGATGCCGGGCGTGCCGCTGGTGACGCGGCGCATGGCCCTGAAAATCGTCGAGGGGTATCTGAGC
TGGTTCGCGCAGCGCGAATTCACGAAACTGAAAAAAGCGCTCGACTGCGACGACGAGGACCTGCGTGAAGCGCAGGCCGT
GATCCGCCAGTGCAATCCGCATCCCGGCGCCGCCTTCGCGTCGAGCGTCTCGGATTACGTCGTACCGGACGTTATTGTCC
GCAAGTCGAAGGGCGGCTGGAGCGTCTCGCTGAACAACGAAGTGATGCCGCGCCTGCGTGTGAACAACGTCTATGCGAAT
TTGCTCAAGCAGGGCAAAGGCGAAGGCCAGATGAGCGCGCAGCTGCAGGAAGCGAAGTGGCTCATTAAAAATATGCGCCA
GCGTTTCGACACGATCCTGCGTGTTTCGCAGGCAATTGTGGAGCGCCAGAAGAACTTTTTCAGCCATGGCGCGGTTGCCA
TGCGTCCCCTTGTGCTTCGCGAGATAGCTGATACACTAGGACTACACGAGAGCACAATTTCTCGCGTGACAACTCAAAAA
TTCATGCTGACCCCGCACGGCATGTTTGAGCTGAAGTATTTCTTCGGAAGCCATGTCGCCACCGAAGCGGGGGGCGAAGC
ATCGTCTACGGCGATACGGGCGCTCATTGCGCAACTGACAGGAGCAGAAGACCCAAAGAACCCATTATCCGACAGCAAGA
TCGCGGAAATGCTCGGTGAACAAGGGATGGTCATTGCGCGCCGCACGGTTGCAAAATACCGCGAGGCACTCAAGATTCCT
CCCGTCAGTCTCCGCAAATCCTTGTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(331-486)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(131-318)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

59.127

100

0.611