Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LPB04_RS01185 Genome accession   NZ_CP062941
Coordinates   287828..288928 (+) Length   366 a.a.
NCBI ID   WP_193687001.1    Uniprot ID   -
Organism   Massilia litorea strain LPB0304     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 282828..293928
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPB04_RS01165 (LPB04_01165) - 283782..285482 (+) 1701 WP_193686997.1 thiamine pyrophosphate-binding protein -
  LPB04_RS01170 (LPB04_01170) - 285517..286455 (-) 939 WP_193686998.1 hypothetical protein -
  LPB04_RS01175 (LPB04_01175) - 286613..287374 (+) 762 WP_193686999.1 DUF4261 domain-containing protein -
  LPB04_RS01180 (LPB04_01180) - 287394..287543 (-) 150 WP_193687000.1 hypothetical protein -
  LPB04_RS01185 (LPB04_01185) pilA 287828..288928 (+) 1101 WP_193687001.1 signal recognition particle-docking protein FtsY Machinery gene
  LPB04_RS01190 (LPB04_01190) - 288935..289609 (+) 675 WP_193687002.1 cell division ATP-binding protein FtsE -
  LPB04_RS01195 (LPB04_01195) ftsX 289606..290526 (+) 921 WP_193687003.1 permease-like cell division protein FtsX -
  LPB04_RS01200 (LPB04_01200) rpoH 291040..291933 (+) 894 WP_193688815.1 RNA polymerase sigma factor RpoH -
  LPB04_RS01205 (LPB04_01205) - 292149..293405 (-) 1257 WP_193687004.1 NAD(P)/FAD-dependent oxidoreductase -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 39004.23 Da        Isoelectric Point: 6.1679

>NTDB_id=492031 LPB04_RS01185 WP_193687001.1 287828..288928(+) (pilA) [Massilia litorea strain LPB0304]
MFSFFKRKKAADTPAPVEVVPAPVVPAPAPAPAPATPALAPAPVTQAVPEADLFIEETAERPTSEAEKKRSWMTRLKAGL
SKTSSNLSLLFVGARIDEDLYEELESALLMSDAGMDATQFLLDSLRRKVKEDKLLDAAAVKAALKTLMIEMLAPLQKPLE
LGRHEPLVMMISGVNGAGKTTTIGKLAKHMQRSDQSVLLAAGDTFRAAAREQLMVWGQRNNVTVISQASGDPAAVAFDAV
QSGKARGVDVVMVDTAGRLPTQLHLMEELKKIKRVIGKGMDGAPHETVLVIDGNTGQNALAQVKAFDDALQLTGLVITKL
DGTAKGGVLAAIARTRPVPVYFIGVGEKLDDLQPFNAEEFVEALLG

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=492031 LPB04_RS01185 WP_193687001.1 287828..288928(+) (pilA) [Massilia litorea strain LPB0304]
ATGTTTAGTTTCTTTAAAAGAAAAAAAGCCGCCGACACACCGGCGCCGGTCGAGGTTGTGCCGGCGCCGGTTGTGCCCGC
CCCTGCGCCCGCCCCGGCGCCTGCTACACCTGCCCTGGCACCTGCCCCGGTAACGCAAGCCGTCCCTGAAGCCGACCTCT
TCATCGAAGAAACGGCCGAACGCCCGACCAGCGAAGCGGAAAAGAAACGGTCCTGGATGACGCGCCTGAAGGCGGGCCTG
TCCAAGACCTCGAGCAATTTGTCGCTGCTGTTCGTCGGCGCGCGCATCGACGAAGACCTGTACGAGGAACTCGAGTCCGC
GCTGCTGATGTCGGATGCCGGCATGGACGCGACCCAGTTTCTTCTCGATAGCCTGCGCCGCAAGGTGAAGGAGGACAAGC
TGCTCGACGCCGCCGCCGTGAAGGCCGCGCTGAAAACGCTGATGATCGAGATGCTCGCGCCCCTGCAAAAGCCGCTGGAA
CTGGGCCGCCATGAGCCGCTCGTGATGATGATTTCCGGCGTCAATGGTGCGGGTAAAACCACCACCATCGGCAAACTCGC
CAAGCACATGCAGCGCTCGGATCAGTCCGTGCTGCTGGCCGCCGGCGACACCTTCCGCGCCGCTGCGCGCGAGCAGTTGA
TGGTCTGGGGCCAGCGCAACAACGTCACCGTGATCTCGCAAGCCTCGGGCGATCCGGCCGCGGTCGCCTTCGACGCGGTG
CAGTCTGGCAAGGCACGCGGCGTTGACGTCGTGATGGTCGATACAGCCGGCCGCCTGCCGACCCAGCTGCACCTGATGGA
AGAACTGAAAAAGATCAAGCGCGTGATCGGCAAGGGCATGGATGGCGCCCCCCACGAAACCGTGCTCGTCATCGACGGCA
ATACCGGCCAGAACGCGCTGGCGCAGGTGAAGGCTTTTGATGACGCGCTGCAGCTGACCGGCCTCGTGATCACCAAGCTC
GACGGCACGGCCAAGGGCGGCGTGCTGGCCGCGATCGCGCGCACGCGTCCGGTGCCGGTGTACTTCATCGGCGTCGGCGA
GAAGCTGGACGACCTGCAGCCTTTCAATGCAGAAGAGTTCGTCGAAGCGCTGCTCGGATAA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(93-145)

SRP54-type protein, GTPase domain (SRP54)

(167-366)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

50.119

100

0.574