Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KHZ70_RS23425 Genome accession   NZ_CP074576
Coordinates   4865865..4866542 (-) Length   225 a.a.
NCBI ID   WP_000178169.1    Uniprot ID   -
Organism   Escherichia coli strain CS18F     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4860865..4871542
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHZ70_RS23410 (KHZ70_23415) chiA 4862223..4864925 (+) 2703 WP_000773176.1 bifunctional chitinase/lysozyme -
  KHZ70_RS23415 (KHZ70_23420) bfd 4865094..4865288 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  KHZ70_RS23420 (KHZ70_23425) bfr 4865360..4865836 (+) 477 WP_000675505.1 bacterioferritin -
  KHZ70_RS23425 (KHZ70_23430) pilD 4865865..4866542 (-) 678 WP_000178169.1 prepilin peptidase GspO Machinery gene
  KHZ70_RS23430 (KHZ70_23435) gspM 4866542..4867003 (-) 462 WP_000597914.1 type II secretion system protein GspM -
  KHZ70_RS23435 (KHZ70_23440) gspL 4867000..4868163 (-) 1164 WP_001115245.1 type II secretion system protein GspL -
  KHZ70_RS23440 (KHZ70_23445) gspK 4868178..4869161 (-) 984 WP_001058528.1 type II secretion system minor pseudopilin GspK -
  KHZ70_RS23445 (KHZ70_23450) gspJ 4869154..4869741 (-) 588 WP_000610629.1 type II secretion system minor pseudopilin GspJ -
  KHZ70_RS23450 (KHZ70_23455) gspI 4869734..4870111 (-) 378 WP_001041738.1 type II secretion system minor pseudopilin GspI -
  KHZ70_RS23455 (KHZ70_23460) gspH 4870108..4870617 (-) 510 WP_001076031.1 type II secretion system minor pseudopilin GspH -
  KHZ70_RS23460 (KHZ70_23465) gspG 4870625..4871062 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24812.67 Da        Isoelectric Point: 7.9585

>NTDB_id=491663 KHZ70_RS23425 WP_000178169.1 4865865..4866542(-) (pilD) [Escherichia coli strain CS18F]
MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=491663 KHZ70_RS23425 WP_000178169.1 4865865..4866542(-) (pilD) [Escherichia coli strain CS18F]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATGGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTAATTGCCTCACTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCGTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.382