Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   KHZ70_RS17765 Genome accession   NZ_CP074576
Coordinates   3657963..3659345 (-) Length   460 a.a.
NCBI ID   WP_001029698.1    Uniprot ID   A0A2T3RLH6
Organism   Escherichia coli strain CS18F     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3652963..3664345
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHZ70_RS17745 (KHZ70_17755) ettA 3654277..3655944 (+) 1668 WP_000046743.1 energy-dependent translational throttle protein EttA -
  KHZ70_RS17750 (KHZ70_17760) nadS 3656000..3656284 (-) 285 WP_000007436.1 NadS family protein -
  KHZ70_RS17755 (KHZ70_17765) - 3656286..3656618 (-) 333 WP_001317608.1 type II toxin-antitoxin system RelE/ParE family toxin -
  KHZ70_RS17760 (KHZ70_17770) nadR 3656710..3657942 (-) 1233 WP_213201914.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  KHZ70_RS17765 (KHZ70_17775) radA/sms 3657963..3659345 (-) 1383 WP_001029698.1 DNA repair protein RadA Machinery gene
  KHZ70_RS17770 (KHZ70_17780) serB 3659394..3660362 (-) 969 WP_001132967.1 phosphoserine phosphatase -
  KHZ70_RS17775 (KHZ70_17785) ytjB 3660468..3661112 (+) 645 WP_000124623.1 YtjB family periplasmic protein -
  KHZ70_RS17780 (KHZ70_17790) lplA 3661140..3662156 (+) 1017 WP_000105834.1 lipoate--protein ligase LplA -
  KHZ70_RS17785 (KHZ70_17795) - 3662188..3662451 (+) 264 WP_000566145.1 helix-turn-helix transcriptional regulator -
  KHZ70_RS17790 (KHZ70_17800) deoD 3662612..3663331 (-) 720 WP_000224879.1 purine-nucleoside phosphorylase -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49442.02 Da        Isoelectric Point: 7.1936

>NTDB_id=491633 KHZ70_RS17765 WP_001029698.1 3657963..3659345(-) (radA/sms) [Escherichia coli strain CS18F]
MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPTVARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTG
FKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDH
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEV
GLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKLSDALSVFDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=491633 KHZ70_RS17765 WP_001029698.1 3657963..3659345(-) (radA/sms) [Escherichia coli strain CS18F]
GTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGCGC
CTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAACGGTGGCGCGTAACGAGCGTCTCAGTGGCT
ATGCCGGTAGCGCCGGAGTGGCAAAAGTCCAGAAACTTTCCGACATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGA
TTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCGGGAAGTGCCATTCTGATTGGCGGTAACCCAGGTGCCGG
GAAATCCACGCTGCTGTTGCAAACGCTGTGCAAACTGGCGCAGCAGATGAAAACGCTGTATGTCACTGGCGAAGAGTCGC
TGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAA
CAAATCTGCTTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGT
ACAATCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGCGTGG
CGATTGTCATGGTGGGGCACGTAACCAAAGATGGCTCGCTGGCTGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCA
GTGCTTCTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCT
GGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTTAGCAACCCTTCCGCCATCTTTTTAAGCCGCGGTGATGAAG
TGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTCAGGCATTGGTCGATCAC
TCGATGATGGCGAACCCACGCCGCGTGGCAGTGGGGCTGGAGCAAAACCGTCTGGCAATCCTGCTGGCGGTGCTGCACCG
TCACGGTGGGTTGCAAATGGCCGATCAGGATGTATTTGTTAACGTGGTCGGCGGCGTGAAGGTGACGGAAACCAGCGCCG
ACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTC
GGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGCCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGGCGGGC
GATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGC
TTAGCGTGTTCGACGACTTATAA

Domains

Predicted by InterProScan.

AAA domain (AAA_25)

(89-225)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T3RLH6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

46.827

99.348

0.465
  radA Streptococcus mitis SK321

43.86

99.13

0.435
  radA Streptococcus pneumoniae D39

43.421

99.13

0.43
  radA Streptococcus pneumoniae TIGR4

43.421

99.13

0.43
  radA Streptococcus mitis NCTC 12261

43.421

99.13

0.43
  radA Streptococcus pneumoniae R6

43.421

99.13

0.43
  radA Streptococcus pneumoniae Rx1

43.421

99.13

0.43